Cargando…

High‐resolution quantitative MRI of multiple sclerosis spinal cord lesions

PURPOSE: Validation of quantitative MR measures for myelin imaging in the postmortem multiple sclerosis spinal cord. METHODS: Four fixed spinal cord samples were imaged first with a 3T clinical MR scanner to identify areas of interest for scanning, and then with a 7T small bore scanner using a multi...

Descripción completa

Detalles Bibliográficos
Autores principales: McDowell, Amy R., Petrova, Natalia, Carassiti, Daniele, Miquel, Marc E., Thomas, David L., Barker, Gareth J., Schmierer, Klaus, Wood, Tobias C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208576/
https://www.ncbi.nlm.nih.gov/pubmed/35014736
http://dx.doi.org/10.1002/mrm.29152
_version_ 1784729762905718784
author McDowell, Amy R.
Petrova, Natalia
Carassiti, Daniele
Miquel, Marc E.
Thomas, David L.
Barker, Gareth J.
Schmierer, Klaus
Wood, Tobias C.
author_facet McDowell, Amy R.
Petrova, Natalia
Carassiti, Daniele
Miquel, Marc E.
Thomas, David L.
Barker, Gareth J.
Schmierer, Klaus
Wood, Tobias C.
author_sort McDowell, Amy R.
collection PubMed
description PURPOSE: Validation of quantitative MR measures for myelin imaging in the postmortem multiple sclerosis spinal cord. METHODS: Four fixed spinal cord samples were imaged first with a 3T clinical MR scanner to identify areas of interest for scanning, and then with a 7T small bore scanner using a multicomponent‐driven equilibrium single‐pulse observation of T(1) and T(2) protocol to produce apparent proton density, T(1), T(2), myelin water, intracellular water, and free‐water fraction maps. After imaging, the cords were sectioned and stained with histological markers (hematoxylin and eosin, myelin basic protein, and neurofilament protein), which were quantitatively compared with the MR maps. RESULTS: Excellent correspondence was found between high‐resolution MR parameter maps and histology, particularly for apparent proton density MRI and myelin basic protein staining. CONCLUSION: High‐resolution quantitative MRI of the spinal cord provides biologically meaningful measures, and could be beneficial to diagnose and track multiple sclerosis lesions in the spinal cord.
format Online
Article
Text
id pubmed-9208576
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-92085762022-06-27 High‐resolution quantitative MRI of multiple sclerosis spinal cord lesions McDowell, Amy R. Petrova, Natalia Carassiti, Daniele Miquel, Marc E. Thomas, David L. Barker, Gareth J. Schmierer, Klaus Wood, Tobias C. Magn Reson Med Technical Notes—Preclinical and Clinical Imaging PURPOSE: Validation of quantitative MR measures for myelin imaging in the postmortem multiple sclerosis spinal cord. METHODS: Four fixed spinal cord samples were imaged first with a 3T clinical MR scanner to identify areas of interest for scanning, and then with a 7T small bore scanner using a multicomponent‐driven equilibrium single‐pulse observation of T(1) and T(2) protocol to produce apparent proton density, T(1), T(2), myelin water, intracellular water, and free‐water fraction maps. After imaging, the cords were sectioned and stained with histological markers (hematoxylin and eosin, myelin basic protein, and neurofilament protein), which were quantitatively compared with the MR maps. RESULTS: Excellent correspondence was found between high‐resolution MR parameter maps and histology, particularly for apparent proton density MRI and myelin basic protein staining. CONCLUSION: High‐resolution quantitative MRI of the spinal cord provides biologically meaningful measures, and could be beneficial to diagnose and track multiple sclerosis lesions in the spinal cord. John Wiley and Sons Inc. 2022-01-11 2022-06 /pmc/articles/PMC9208576/ /pubmed/35014736 http://dx.doi.org/10.1002/mrm.29152 Text en © 2022 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals LLC on behalf of International Society for Magnetic Resonance in Medicine. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Notes—Preclinical and Clinical Imaging
McDowell, Amy R.
Petrova, Natalia
Carassiti, Daniele
Miquel, Marc E.
Thomas, David L.
Barker, Gareth J.
Schmierer, Klaus
Wood, Tobias C.
High‐resolution quantitative MRI of multiple sclerosis spinal cord lesions
title High‐resolution quantitative MRI of multiple sclerosis spinal cord lesions
title_full High‐resolution quantitative MRI of multiple sclerosis spinal cord lesions
title_fullStr High‐resolution quantitative MRI of multiple sclerosis spinal cord lesions
title_full_unstemmed High‐resolution quantitative MRI of multiple sclerosis spinal cord lesions
title_short High‐resolution quantitative MRI of multiple sclerosis spinal cord lesions
title_sort high‐resolution quantitative mri of multiple sclerosis spinal cord lesions
topic Technical Notes—Preclinical and Clinical Imaging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208576/
https://www.ncbi.nlm.nih.gov/pubmed/35014736
http://dx.doi.org/10.1002/mrm.29152
work_keys_str_mv AT mcdowellamyr highresolutionquantitativemriofmultiplesclerosisspinalcordlesions
AT petrovanatalia highresolutionquantitativemriofmultiplesclerosisspinalcordlesions
AT carassitidaniele highresolutionquantitativemriofmultiplesclerosisspinalcordlesions
AT miquelmarce highresolutionquantitativemriofmultiplesclerosisspinalcordlesions
AT thomasdavidl highresolutionquantitativemriofmultiplesclerosisspinalcordlesions
AT barkergarethj highresolutionquantitativemriofmultiplesclerosisspinalcordlesions
AT schmiererklaus highresolutionquantitativemriofmultiplesclerosisspinalcordlesions
AT woodtobiasc highresolutionquantitativemriofmultiplesclerosisspinalcordlesions