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The role of surface adhesion on the macroscopic wrinkling of biofilms

Biofilms, bacterial communities of cells encased by a self-produced matrix, exhibit a variety of three-dimensional structures. Specifically, channel networks formed within the bulk of the biofilm have been identified to play an important role in the colonies' viability by promoting the transpor...

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Detalles Bibliográficos
Autores principales: Geisel, Steffen, Secchi, Eleonora, Vermant, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208754/
https://www.ncbi.nlm.nih.gov/pubmed/35723588
http://dx.doi.org/10.7554/eLife.76027
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author Geisel, Steffen
Secchi, Eleonora
Vermant, Jan
author_facet Geisel, Steffen
Secchi, Eleonora
Vermant, Jan
author_sort Geisel, Steffen
collection PubMed
description Biofilms, bacterial communities of cells encased by a self-produced matrix, exhibit a variety of three-dimensional structures. Specifically, channel networks formed within the bulk of the biofilm have been identified to play an important role in the colonies' viability by promoting the transport of nutrients and chemicals. Here, we study channel formation and focus on the role of the adhesion of the biofilm matrix to the substrate in Pseudomonas aeruginosa biofilms grown under constant flow in microfluidic channels. We perform phase contrast and confocal laser scanning microscopy to examine the development of the biofilm structure as a function of the substrates' surface energy. The formation of the wrinkles and folds is triggered by a mechanical buckling instability, controlled by biofilm growth rate and the film’s adhesion to the substrate. The three-dimensional folding gives rise to hollow channels that rapidly increase the effective volume occupied by the biofilm and facilitate bacterial movement inside them. The experiments and analysis on mechanical instabilities for the relevant case of a bacterial biofilm grown during flow enable us to predict and control the biofilm morphology.
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spelling pubmed-92087542022-06-21 The role of surface adhesion on the macroscopic wrinkling of biofilms Geisel, Steffen Secchi, Eleonora Vermant, Jan eLife Physics of Living Systems Biofilms, bacterial communities of cells encased by a self-produced matrix, exhibit a variety of three-dimensional structures. Specifically, channel networks formed within the bulk of the biofilm have been identified to play an important role in the colonies' viability by promoting the transport of nutrients and chemicals. Here, we study channel formation and focus on the role of the adhesion of the biofilm matrix to the substrate in Pseudomonas aeruginosa biofilms grown under constant flow in microfluidic channels. We perform phase contrast and confocal laser scanning microscopy to examine the development of the biofilm structure as a function of the substrates' surface energy. The formation of the wrinkles and folds is triggered by a mechanical buckling instability, controlled by biofilm growth rate and the film’s adhesion to the substrate. The three-dimensional folding gives rise to hollow channels that rapidly increase the effective volume occupied by the biofilm and facilitate bacterial movement inside them. The experiments and analysis on mechanical instabilities for the relevant case of a bacterial biofilm grown during flow enable us to predict and control the biofilm morphology. eLife Sciences Publications, Ltd 2022-06-20 /pmc/articles/PMC9208754/ /pubmed/35723588 http://dx.doi.org/10.7554/eLife.76027 Text en © 2022, Geisel et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Physics of Living Systems
Geisel, Steffen
Secchi, Eleonora
Vermant, Jan
The role of surface adhesion on the macroscopic wrinkling of biofilms
title The role of surface adhesion on the macroscopic wrinkling of biofilms
title_full The role of surface adhesion on the macroscopic wrinkling of biofilms
title_fullStr The role of surface adhesion on the macroscopic wrinkling of biofilms
title_full_unstemmed The role of surface adhesion on the macroscopic wrinkling of biofilms
title_short The role of surface adhesion on the macroscopic wrinkling of biofilms
title_sort role of surface adhesion on the macroscopic wrinkling of biofilms
topic Physics of Living Systems
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208754/
https://www.ncbi.nlm.nih.gov/pubmed/35723588
http://dx.doi.org/10.7554/eLife.76027
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