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Reducing expression of TaOTUB1s decreases tiller number in wheat
Tiller number is an important agronomic trait that affects crop yield. The roles of OTUB1 in regulating tiller numbers in rice have been reported. However, the roles of OTUB1 in wheat remain elusive. In this study, TaOTUB1s were identified in wheat. TaOTUB1 proteins were localized in the nucleus and...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208779/ https://www.ncbi.nlm.nih.gov/pubmed/34968411 http://dx.doi.org/10.1080/15592324.2021.2018217 |
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author | Jiang, Deng Ji Hao, Xiao Hua Zhang, Jing Tang, Heng Wang, Fang |
author_facet | Jiang, Deng Ji Hao, Xiao Hua Zhang, Jing Tang, Heng Wang, Fang |
author_sort | Jiang, Deng Ji |
collection | PubMed |
description | Tiller number is an important agronomic trait that affects crop yield. The roles of OTUB1 in regulating tiller numbers in rice have been reported. However, the roles of OTUB1 in wheat remain elusive. In this study, TaOTUB1s were identified in wheat. TaOTUB1 proteins were localized in the nucleus and cytoplasm. Compared with wild-type Fielder, TaOTUB1-RNAi transgenic wheat plants had fewer tillers. Similar to OTUB1 in rice, the yeast double hybrid indicated that the TaOTUB1-A protein could interact with TaSPL17 and TaUBC13 proteins. The results of quantitative real-time polymerase chain reaction revealed that the expression levels of TaOTUB1s decreased while those of TaSPL17 significantly improved in TaOTUB1-RNAi lines. These findings suggested that TaOTUB1s influenced tiller number in wheat. |
format | Online Article Text |
id | pubmed-9208779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-92087792022-06-21 Reducing expression of TaOTUB1s decreases tiller number in wheat Jiang, Deng Ji Hao, Xiao Hua Zhang, Jing Tang, Heng Wang, Fang Plant Signal Behav Research Paper Tiller number is an important agronomic trait that affects crop yield. The roles of OTUB1 in regulating tiller numbers in rice have been reported. However, the roles of OTUB1 in wheat remain elusive. In this study, TaOTUB1s were identified in wheat. TaOTUB1 proteins were localized in the nucleus and cytoplasm. Compared with wild-type Fielder, TaOTUB1-RNAi transgenic wheat plants had fewer tillers. Similar to OTUB1 in rice, the yeast double hybrid indicated that the TaOTUB1-A protein could interact with TaSPL17 and TaUBC13 proteins. The results of quantitative real-time polymerase chain reaction revealed that the expression levels of TaOTUB1s decreased while those of TaSPL17 significantly improved in TaOTUB1-RNAi lines. These findings suggested that TaOTUB1s influenced tiller number in wheat. Taylor & Francis 2021-12-30 /pmc/articles/PMC9208779/ /pubmed/34968411 http://dx.doi.org/10.1080/15592324.2021.2018217 Text en © 2021 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Jiang, Deng Ji Hao, Xiao Hua Zhang, Jing Tang, Heng Wang, Fang Reducing expression of TaOTUB1s decreases tiller number in wheat |
title | Reducing expression of TaOTUB1s decreases tiller number in wheat |
title_full | Reducing expression of TaOTUB1s decreases tiller number in wheat |
title_fullStr | Reducing expression of TaOTUB1s decreases tiller number in wheat |
title_full_unstemmed | Reducing expression of TaOTUB1s decreases tiller number in wheat |
title_short | Reducing expression of TaOTUB1s decreases tiller number in wheat |
title_sort | reducing expression of taotub1s decreases tiller number in wheat |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208779/ https://www.ncbi.nlm.nih.gov/pubmed/34968411 http://dx.doi.org/10.1080/15592324.2021.2018217 |
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