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235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS)

OBJECTIVES/GOALS: Current approaches to drug development for the aggressive triple negative breast cancer rely on current 2D and 3D in vitro models which have limited capabilities. We have developed a translational microphysiological system that can maintain the human breast microenvironment to capt...

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Autores principales: Hebert, Katherine L., Nguyen, Khoa, Cheng, Thomas, Alzoubi, Madlin, Elliott, Steven, Collins-Burow, Bridgette, Martin, Elizabeth, Lau, Frank, Burow, Matthew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209041/
http://dx.doi.org/10.1017/cts.2022.124
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author Hebert, Katherine L.
Nguyen, Khoa
Cheng, Thomas
Alzoubi, Madlin
Elliott, Steven
Collins-Burow, Bridgette
Martin, Elizabeth
Lau, Frank
Burow, Matthew
author_facet Hebert, Katherine L.
Nguyen, Khoa
Cheng, Thomas
Alzoubi, Madlin
Elliott, Steven
Collins-Burow, Bridgette
Martin, Elizabeth
Lau, Frank
Burow, Matthew
author_sort Hebert, Katherine L.
collection PubMed
description OBJECTIVES/GOALS: Current approaches to drug development for the aggressive triple negative breast cancer rely on current 2D and 3D in vitro models which have limited capabilities. We have developed a translational microphysiological system that can maintain the human breast microenvironment to capture the complex interaction with the tumor microenvironment. METHODS/STUDY POPULATION: Three different TNBC cell lines were seeded in BC-MPS: MDA-MB-231 parental cell line, MDA-MB-231wiht the gene, LKB1 overexpressed, which is a tumor suppressor, and MDA-MB-231 with the enzyme, ERK5, an enzyme associated with increased metastasis and drug resistance, knocked out. These three TNBC cell lines were cultured in a standard 2D 96-well plate and in BC-MPS. Time-lapse videos were taken to track cellular mobility. RNA-sequencing was performed to compare different expression levels of various cancer related genes of the cell lines cultured in standard 2D and BC-MPS. RESULTS/ANTICIPATED RESULTS: The LKB1 overexpressed MDA-MB-231 and the ERK5-ko MDA-MB-231 cell lines are expected to have decreased mobility compared to the parental cells. The cell lines are expected to have increased expression of cancer related genes when cultured in BC-MPS than when cultured in standard 2D due to the presence of human breast tissue. DISCUSSION/SIGNIFICANCE: BC-MPS is a promising new translational MPS that facilitates studying long term interactions between real human breast tissue and cancer cells. The BC-MPS systems ability to support the growth of established cell lines has been demonstrated. Future studies will focus on developing the model for personalized medicine.
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spelling pubmed-92090412022-07-01 235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS) Hebert, Katherine L. Nguyen, Khoa Cheng, Thomas Alzoubi, Madlin Elliott, Steven Collins-Burow, Bridgette Martin, Elizabeth Lau, Frank Burow, Matthew J Clin Transl Sci Valued Approaches OBJECTIVES/GOALS: Current approaches to drug development for the aggressive triple negative breast cancer rely on current 2D and 3D in vitro models which have limited capabilities. We have developed a translational microphysiological system that can maintain the human breast microenvironment to capture the complex interaction with the tumor microenvironment. METHODS/STUDY POPULATION: Three different TNBC cell lines were seeded in BC-MPS: MDA-MB-231 parental cell line, MDA-MB-231wiht the gene, LKB1 overexpressed, which is a tumor suppressor, and MDA-MB-231 with the enzyme, ERK5, an enzyme associated with increased metastasis and drug resistance, knocked out. These three TNBC cell lines were cultured in a standard 2D 96-well plate and in BC-MPS. Time-lapse videos were taken to track cellular mobility. RNA-sequencing was performed to compare different expression levels of various cancer related genes of the cell lines cultured in standard 2D and BC-MPS. RESULTS/ANTICIPATED RESULTS: The LKB1 overexpressed MDA-MB-231 and the ERK5-ko MDA-MB-231 cell lines are expected to have decreased mobility compared to the parental cells. The cell lines are expected to have increased expression of cancer related genes when cultured in BC-MPS than when cultured in standard 2D due to the presence of human breast tissue. DISCUSSION/SIGNIFICANCE: BC-MPS is a promising new translational MPS that facilitates studying long term interactions between real human breast tissue and cancer cells. The BC-MPS systems ability to support the growth of established cell lines has been demonstrated. Future studies will focus on developing the model for personalized medicine. Cambridge University Press 2022-04-19 /pmc/articles/PMC9209041/ http://dx.doi.org/10.1017/cts.2022.124 Text en © The Association for Clinical and Translational Science 2022 https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is unaltered and is properly cited. The written permission of Cambridge University Press must be obtained for commercial re-use or in order to create a derivative work.
spellingShingle Valued Approaches
Hebert, Katherine L.
Nguyen, Khoa
Cheng, Thomas
Alzoubi, Madlin
Elliott, Steven
Collins-Burow, Bridgette
Martin, Elizabeth
Lau, Frank
Burow, Matthew
235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS)
title 235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS)
title_full 235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS)
title_fullStr 235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS)
title_full_unstemmed 235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS)
title_short 235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS)
title_sort 235 analysiss of tnbc cell lines cultured a novel translational breast cancer microphysiological system (bc-mps)
topic Valued Approaches
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209041/
http://dx.doi.org/10.1017/cts.2022.124
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