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351 Loop-mediated isothermal amplification for the detection of cytomegalovirus infection and drug resistance at the point of care
OBJECTIVES/GOALS: To develop a loop-mediated isothermal amplification (LAMP) assay for the detection of cytomegalovirus (CMV) infection and drug resistance. METHODS/STUDY POPULATION: We designed core and loop primers sets utilizing the NEB LAMP Primer Design Tool. Each set contained four or six prim...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cambridge University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209238/ http://dx.doi.org/10.1017/cts.2022.199 |
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author | Medina-Rivera, Melisa Westblade, Lars F. Erickson, David Mehta, Saurabh |
author_facet | Medina-Rivera, Melisa Westblade, Lars F. Erickson, David Mehta, Saurabh |
author_sort | Medina-Rivera, Melisa |
collection | PubMed |
description | OBJECTIVES/GOALS: To develop a loop-mediated isothermal amplification (LAMP) assay for the detection of cytomegalovirus (CMV) infection and drug resistance. METHODS/STUDY POPULATION: We designed core and loop primers sets utilizing the NEB LAMP Primer Design Tool. Each set contained four or six primers targeting major-immediate early genes – essential for viral entry/replication – or regions known to confer resistance to the antiviral drug ganciclovir. Optimization of reactions conditions was achieved employing DNA reference materials. Reactions were visualized through a change in color as amplification reactions accumulated. Successful reaction conditions were selected based on specific amplification products in less than 60 minutes. Limits of detection were evaluated as the main performance outcome. RESULTS/ANTICIPATED RESULTS: Genomic data were extracted and used to design a series of LAMP primers (48 total) that aimed to detect specific genomic regions of CMV. Using this strategy, we successfully designed and identified eight primer sets that showed high 100% sensitivity and 100 % specificity, when detecting > 1.00 x 10^5 copies/mL of CMV gDNA. We are in the process of characterizing a new set of primers to determine the diagnostic utility of a LAMP assay in detecting selected single-nucleotide mutations at the UL97 loci. The expected outcomes at completion include: (1) the identification of LAMP primers to detect drug-resistance mutants, (2) defining optimal conditions for successful reactions, and (3) determining limits of detection for subsequent validation with clinical specimens. DISCUSSION/SIGNIFICANCE: CMV infection remains one of the most dangerous infectious agents for immunocompromised hosts, newborns, and unborn children. This study will describe a proof-of-concept LAMP assay for the genotypic detection of drug resistance in CMV-infected individuals and hence, create new avenues for selection of effective therapies to treat CMV disease. |
format | Online Article Text |
id | pubmed-9209238 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Cambridge University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-92092382022-07-01 351 Loop-mediated isothermal amplification for the detection of cytomegalovirus infection and drug resistance at the point of care Medina-Rivera, Melisa Westblade, Lars F. Erickson, David Mehta, Saurabh J Clin Transl Sci Valued Approaches OBJECTIVES/GOALS: To develop a loop-mediated isothermal amplification (LAMP) assay for the detection of cytomegalovirus (CMV) infection and drug resistance. METHODS/STUDY POPULATION: We designed core and loop primers sets utilizing the NEB LAMP Primer Design Tool. Each set contained four or six primers targeting major-immediate early genes – essential for viral entry/replication – or regions known to confer resistance to the antiviral drug ganciclovir. Optimization of reactions conditions was achieved employing DNA reference materials. Reactions were visualized through a change in color as amplification reactions accumulated. Successful reaction conditions were selected based on specific amplification products in less than 60 minutes. Limits of detection were evaluated as the main performance outcome. RESULTS/ANTICIPATED RESULTS: Genomic data were extracted and used to design a series of LAMP primers (48 total) that aimed to detect specific genomic regions of CMV. Using this strategy, we successfully designed and identified eight primer sets that showed high 100% sensitivity and 100 % specificity, when detecting > 1.00 x 10^5 copies/mL of CMV gDNA. We are in the process of characterizing a new set of primers to determine the diagnostic utility of a LAMP assay in detecting selected single-nucleotide mutations at the UL97 loci. The expected outcomes at completion include: (1) the identification of LAMP primers to detect drug-resistance mutants, (2) defining optimal conditions for successful reactions, and (3) determining limits of detection for subsequent validation with clinical specimens. DISCUSSION/SIGNIFICANCE: CMV infection remains one of the most dangerous infectious agents for immunocompromised hosts, newborns, and unborn children. This study will describe a proof-of-concept LAMP assay for the genotypic detection of drug resistance in CMV-infected individuals and hence, create new avenues for selection of effective therapies to treat CMV disease. Cambridge University Press 2022-04-19 /pmc/articles/PMC9209238/ http://dx.doi.org/10.1017/cts.2022.199 Text en © The Association for Clinical and Translational Science 2022 https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is unaltered and is properly cited. The written permission of Cambridge University Press must be obtained for commercial re-use or in order to create a derivative work. |
spellingShingle | Valued Approaches Medina-Rivera, Melisa Westblade, Lars F. Erickson, David Mehta, Saurabh 351 Loop-mediated isothermal amplification for the detection of cytomegalovirus infection and drug resistance at the point of care |
title | 351 Loop-mediated isothermal amplification for the detection of cytomegalovirus infection and drug resistance at the point of care |
title_full | 351 Loop-mediated isothermal amplification for the detection of cytomegalovirus infection and drug resistance at the point of care |
title_fullStr | 351 Loop-mediated isothermal amplification for the detection of cytomegalovirus infection and drug resistance at the point of care |
title_full_unstemmed | 351 Loop-mediated isothermal amplification for the detection of cytomegalovirus infection and drug resistance at the point of care |
title_short | 351 Loop-mediated isothermal amplification for the detection of cytomegalovirus infection and drug resistance at the point of care |
title_sort | 351 loop-mediated isothermal amplification for the detection of cytomegalovirus infection and drug resistance at the point of care |
topic | Valued Approaches |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209238/ http://dx.doi.org/10.1017/cts.2022.199 |
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