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Industrialized GMP Production of CD34(+) Cells (ProtheraCytes®) at Clinical Scale for Treatment of Ischemic Cardiac Diseases Is Feasible and Safe

Regenerative medicine now needs to pass a crucial turning point, from academic research to the market. Several sources/types of cells have been experimented with, more or less successfully. CD34(+) cells have demonstrated multipotent or even pluripotent capacities, making them good candidates for re...

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Autores principales: Hénon, Philippe, Kowalczyk, Marc, Aries, Anne, Vignon, Christine, Trébuchet, Guillaume, Lahlil, Rachid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209364/
https://www.ncbi.nlm.nih.gov/pubmed/35420389
http://dx.doi.org/10.1007/s12015-022-10373-5
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author Hénon, Philippe
Kowalczyk, Marc
Aries, Anne
Vignon, Christine
Trébuchet, Guillaume
Lahlil, Rachid
author_facet Hénon, Philippe
Kowalczyk, Marc
Aries, Anne
Vignon, Christine
Trébuchet, Guillaume
Lahlil, Rachid
author_sort Hénon, Philippe
collection PubMed
description Regenerative medicine now needs to pass a crucial turning point, from academic research to the market. Several sources/types of cells have been experimented with, more or less successfully. CD34(+) cells have demonstrated multipotent or even pluripotent capacities, making them good candidates for regenerative medicine, particularly for treating heart diseases. Strongly encouraged by the results we achieved in a pilot study using CD34(+) stem cells in patients with poor-prognosis acute myocardial infarcts (AMIs), we soon began the development of an industrialized platform making use of a closed automated device (StemXpand(®)) and a disposable kit (StemPack(®)) for the large-scale expansion of CD34(+) cells with reproducible good manufacturing practice (GMP). This scalable platform can produce expanded CD34(+) cells (ProtheraCytes(®)) of sufficient quality that, interestingly, express early markers of the cardiac and endothelial pathways and early cardiac-mesoderm markers. They also contain CD34(+) pluripotent cells characterized as very small embryonic-like stem cells (VSELs), capable of differentiating under appropriate stimuli into different tissue lineages, including endothelial and cardiomyocytic ones.
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spelling pubmed-92093642022-06-22 Industrialized GMP Production of CD34(+) Cells (ProtheraCytes®) at Clinical Scale for Treatment of Ischemic Cardiac Diseases Is Feasible and Safe Hénon, Philippe Kowalczyk, Marc Aries, Anne Vignon, Christine Trébuchet, Guillaume Lahlil, Rachid Stem Cell Rev Rep Article Regenerative medicine now needs to pass a crucial turning point, from academic research to the market. Several sources/types of cells have been experimented with, more or less successfully. CD34(+) cells have demonstrated multipotent or even pluripotent capacities, making them good candidates for regenerative medicine, particularly for treating heart diseases. Strongly encouraged by the results we achieved in a pilot study using CD34(+) stem cells in patients with poor-prognosis acute myocardial infarcts (AMIs), we soon began the development of an industrialized platform making use of a closed automated device (StemXpand(®)) and a disposable kit (StemPack(®)) for the large-scale expansion of CD34(+) cells with reproducible good manufacturing practice (GMP). This scalable platform can produce expanded CD34(+) cells (ProtheraCytes(®)) of sufficient quality that, interestingly, express early markers of the cardiac and endothelial pathways and early cardiac-mesoderm markers. They also contain CD34(+) pluripotent cells characterized as very small embryonic-like stem cells (VSELs), capable of differentiating under appropriate stimuli into different tissue lineages, including endothelial and cardiomyocytic ones. Springer US 2022-04-14 2022 /pmc/articles/PMC9209364/ /pubmed/35420389 http://dx.doi.org/10.1007/s12015-022-10373-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hénon, Philippe
Kowalczyk, Marc
Aries, Anne
Vignon, Christine
Trébuchet, Guillaume
Lahlil, Rachid
Industrialized GMP Production of CD34(+) Cells (ProtheraCytes®) at Clinical Scale for Treatment of Ischemic Cardiac Diseases Is Feasible and Safe
title Industrialized GMP Production of CD34(+) Cells (ProtheraCytes®) at Clinical Scale for Treatment of Ischemic Cardiac Diseases Is Feasible and Safe
title_full Industrialized GMP Production of CD34(+) Cells (ProtheraCytes®) at Clinical Scale for Treatment of Ischemic Cardiac Diseases Is Feasible and Safe
title_fullStr Industrialized GMP Production of CD34(+) Cells (ProtheraCytes®) at Clinical Scale for Treatment of Ischemic Cardiac Diseases Is Feasible and Safe
title_full_unstemmed Industrialized GMP Production of CD34(+) Cells (ProtheraCytes®) at Clinical Scale for Treatment of Ischemic Cardiac Diseases Is Feasible and Safe
title_short Industrialized GMP Production of CD34(+) Cells (ProtheraCytes®) at Clinical Scale for Treatment of Ischemic Cardiac Diseases Is Feasible and Safe
title_sort industrialized gmp production of cd34(+) cells (protheracytes®) at clinical scale for treatment of ischemic cardiac diseases is feasible and safe
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209364/
https://www.ncbi.nlm.nih.gov/pubmed/35420389
http://dx.doi.org/10.1007/s12015-022-10373-5
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