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The role of pancreas to improve hyperglycemia in STZ-induced diabetic rats by thiamine disulfide

BACKGROUND: The present study investigated the effect of thiamine disulfide (TD) on the pancreas in terms of hyperglycemia improvement and insulin sensitivity increase in diabetic male rats. We also aimed to study the function of Pdx1 (pancreatic and duodenal homeobox 1) and Glut2 (glucose transport...

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Autores principales: Rad, Mahtab Ghanbari, Sharifi, Mohammadreza, Meamar, Rokhsareh, Soltani, Nepton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209469/
https://www.ncbi.nlm.nih.gov/pubmed/35725834
http://dx.doi.org/10.1038/s41387-022-00211-5
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author Rad, Mahtab Ghanbari
Sharifi, Mohammadreza
Meamar, Rokhsareh
Soltani, Nepton
author_facet Rad, Mahtab Ghanbari
Sharifi, Mohammadreza
Meamar, Rokhsareh
Soltani, Nepton
author_sort Rad, Mahtab Ghanbari
collection PubMed
description BACKGROUND: The present study investigated the effect of thiamine disulfide (TD) on the pancreas in terms of hyperglycemia improvement and insulin sensitivity increase in diabetic male rats. We also aimed to study the function of Pdx1 (pancreatic and duodenal homeobox 1) and Glut2 (glucose transporter 2) genes in pancreatic tissue. METHODS: Type 1 diabetes was induced through injection of 60 mg/kg streptozotocin (STZ). The diabetic rats were divided into four groups, namely diabetic control (DC), diabetic treated with thiamine disulfide (D-TD), diabetic treated with insulin (D-insulin), and diabetic treated with TD and insulin (D-insulin+TD). The non-diabetic (NDC) and diabetic groups received a normal diet (14 weeks). Blood glucose level and body weight were measured weekly; insulin tolerance test (ITT) and glucagon tolerance test (GTT) were performed in the last month of the study. The level of serum insulin and glucagon were measured monthly and a hyperglycemic clamp (Insulin Infusion rate (IIR)) was done for all the groups. Pancreas tissue was isolated so that Pdx1and Glut2 genes expression could be measured. RESULTS: We observed that TD therapy decreased blood glucose level, ITT, and serum glucagon levels in comparison with those of the DC group; it also increased serum insulin levels, IIR, and expression of Pdx1 and Glut2 genes in comparison with those of the DC group. CONCLUSION: Administration of TD could improve hyperglycemia in type 1 diabetic animals through improved pancreas function. Therefore, not only does TD have a significant effect on controlling and reducing hyperglycemia in diabetes, but it also has the potential to decrease the dose of insulin administration.
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spelling pubmed-92094692022-06-22 The role of pancreas to improve hyperglycemia in STZ-induced diabetic rats by thiamine disulfide Rad, Mahtab Ghanbari Sharifi, Mohammadreza Meamar, Rokhsareh Soltani, Nepton Nutr Diabetes Article BACKGROUND: The present study investigated the effect of thiamine disulfide (TD) on the pancreas in terms of hyperglycemia improvement and insulin sensitivity increase in diabetic male rats. We also aimed to study the function of Pdx1 (pancreatic and duodenal homeobox 1) and Glut2 (glucose transporter 2) genes in pancreatic tissue. METHODS: Type 1 diabetes was induced through injection of 60 mg/kg streptozotocin (STZ). The diabetic rats were divided into four groups, namely diabetic control (DC), diabetic treated with thiamine disulfide (D-TD), diabetic treated with insulin (D-insulin), and diabetic treated with TD and insulin (D-insulin+TD). The non-diabetic (NDC) and diabetic groups received a normal diet (14 weeks). Blood glucose level and body weight were measured weekly; insulin tolerance test (ITT) and glucagon tolerance test (GTT) were performed in the last month of the study. The level of serum insulin and glucagon were measured monthly and a hyperglycemic clamp (Insulin Infusion rate (IIR)) was done for all the groups. Pancreas tissue was isolated so that Pdx1and Glut2 genes expression could be measured. RESULTS: We observed that TD therapy decreased blood glucose level, ITT, and serum glucagon levels in comparison with those of the DC group; it also increased serum insulin levels, IIR, and expression of Pdx1 and Glut2 genes in comparison with those of the DC group. CONCLUSION: Administration of TD could improve hyperglycemia in type 1 diabetic animals through improved pancreas function. Therefore, not only does TD have a significant effect on controlling and reducing hyperglycemia in diabetes, but it also has the potential to decrease the dose of insulin administration. Nature Publishing Group UK 2022-06-20 /pmc/articles/PMC9209469/ /pubmed/35725834 http://dx.doi.org/10.1038/s41387-022-00211-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Rad, Mahtab Ghanbari
Sharifi, Mohammadreza
Meamar, Rokhsareh
Soltani, Nepton
The role of pancreas to improve hyperglycemia in STZ-induced diabetic rats by thiamine disulfide
title The role of pancreas to improve hyperglycemia in STZ-induced diabetic rats by thiamine disulfide
title_full The role of pancreas to improve hyperglycemia in STZ-induced diabetic rats by thiamine disulfide
title_fullStr The role of pancreas to improve hyperglycemia in STZ-induced diabetic rats by thiamine disulfide
title_full_unstemmed The role of pancreas to improve hyperglycemia in STZ-induced diabetic rats by thiamine disulfide
title_short The role of pancreas to improve hyperglycemia in STZ-induced diabetic rats by thiamine disulfide
title_sort role of pancreas to improve hyperglycemia in stz-induced diabetic rats by thiamine disulfide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209469/
https://www.ncbi.nlm.nih.gov/pubmed/35725834
http://dx.doi.org/10.1038/s41387-022-00211-5
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