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Copper-Nanocoated Ultra-Small Cells in Grain Boundaries Inside an Extinct Vent Chimney
Chemosynthetic organisms flourish around deep-sea hydrothermal vents where energy-rich fluids are emitted from metal sulfide chimneys. However, microbial life hosted in mineral assemblages in extinct chimneys lacking fluid venting remains largely unknown. The interior of extinct chimneys remains ano...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209642/ https://www.ncbi.nlm.nih.gov/pubmed/35747369 http://dx.doi.org/10.3389/fmicb.2022.864205 |
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author | Takamiya, Hinako Kouduka, Mariko Furutani, Hitoshi Mukai, Hiroki Nakagawa, Kaoru Yamamoto, Takushi Kato, Shingo Kodama, Yu Tomioka, Naotaka Ito, Motoo Suzuki, Yohey |
author_facet | Takamiya, Hinako Kouduka, Mariko Furutani, Hitoshi Mukai, Hiroki Nakagawa, Kaoru Yamamoto, Takushi Kato, Shingo Kodama, Yu Tomioka, Naotaka Ito, Motoo Suzuki, Yohey |
author_sort | Takamiya, Hinako |
collection | PubMed |
description | Chemosynthetic organisms flourish around deep-sea hydrothermal vents where energy-rich fluids are emitted from metal sulfide chimneys. However, microbial life hosted in mineral assemblages in extinct chimneys lacking fluid venting remains largely unknown. The interior of extinct chimneys remains anoxic where the percolation of oxygenated seawater is limited within tightly packed metal sulfide grains. Given the scarcity of photosynthetic organics in deep seawater, anaerobic microbes might inhabit the grain boundaries energetically depending on substrates derived from rock-water interactions. In this study, we reported ultra-small cells directly visualized in grain boundaries of CuFeS(2) inside an extinct metal sulfide chimney from the southern Mariana Trough. Nanoscale solid analyses reveal that ultra-small cells are coated with Cu(2)O nanocrystals in grain boundaries enriched with C, N, and P. In situ spectroscopic and spectrometric characterizations demonstrate the distribution of organics with amide groups and a large molecular organic compound in the grain boundaries. We inferred that the ultra-small cells are anaerobes because of the fast dissolution of Cu(2)O nanocrystals in oxygenated solution. This Cu(2)O property also excludes the possibility of microbial contamination from ambient seawater during sampling. It is shown by 16S rRNA gene sequence analysis that the chimney interior is dominated by Pacearchaeota known to have anaerobic metabolisms and ultra-small cells. Our results support the potential existence of photosynthesis-independent microbial ecosystems in grain boundaries in submarine metal sulfides deposits on the early Earth. |
format | Online Article Text |
id | pubmed-9209642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92096422022-06-22 Copper-Nanocoated Ultra-Small Cells in Grain Boundaries Inside an Extinct Vent Chimney Takamiya, Hinako Kouduka, Mariko Furutani, Hitoshi Mukai, Hiroki Nakagawa, Kaoru Yamamoto, Takushi Kato, Shingo Kodama, Yu Tomioka, Naotaka Ito, Motoo Suzuki, Yohey Front Microbiol Microbiology Chemosynthetic organisms flourish around deep-sea hydrothermal vents where energy-rich fluids are emitted from metal sulfide chimneys. However, microbial life hosted in mineral assemblages in extinct chimneys lacking fluid venting remains largely unknown. The interior of extinct chimneys remains anoxic where the percolation of oxygenated seawater is limited within tightly packed metal sulfide grains. Given the scarcity of photosynthetic organics in deep seawater, anaerobic microbes might inhabit the grain boundaries energetically depending on substrates derived from rock-water interactions. In this study, we reported ultra-small cells directly visualized in grain boundaries of CuFeS(2) inside an extinct metal sulfide chimney from the southern Mariana Trough. Nanoscale solid analyses reveal that ultra-small cells are coated with Cu(2)O nanocrystals in grain boundaries enriched with C, N, and P. In situ spectroscopic and spectrometric characterizations demonstrate the distribution of organics with amide groups and a large molecular organic compound in the grain boundaries. We inferred that the ultra-small cells are anaerobes because of the fast dissolution of Cu(2)O nanocrystals in oxygenated solution. This Cu(2)O property also excludes the possibility of microbial contamination from ambient seawater during sampling. It is shown by 16S rRNA gene sequence analysis that the chimney interior is dominated by Pacearchaeota known to have anaerobic metabolisms and ultra-small cells. Our results support the potential existence of photosynthesis-independent microbial ecosystems in grain boundaries in submarine metal sulfides deposits on the early Earth. Frontiers Media S.A. 2022-06-07 /pmc/articles/PMC9209642/ /pubmed/35747369 http://dx.doi.org/10.3389/fmicb.2022.864205 Text en Copyright © 2022 Takamiya, Kouduka, Furutani, Mukai, Nakagawa, Yamamoto, Kato, Kodama, Tomioka, Ito and Suzuki. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Takamiya, Hinako Kouduka, Mariko Furutani, Hitoshi Mukai, Hiroki Nakagawa, Kaoru Yamamoto, Takushi Kato, Shingo Kodama, Yu Tomioka, Naotaka Ito, Motoo Suzuki, Yohey Copper-Nanocoated Ultra-Small Cells in Grain Boundaries Inside an Extinct Vent Chimney |
title | Copper-Nanocoated Ultra-Small Cells in Grain Boundaries Inside an Extinct Vent Chimney |
title_full | Copper-Nanocoated Ultra-Small Cells in Grain Boundaries Inside an Extinct Vent Chimney |
title_fullStr | Copper-Nanocoated Ultra-Small Cells in Grain Boundaries Inside an Extinct Vent Chimney |
title_full_unstemmed | Copper-Nanocoated Ultra-Small Cells in Grain Boundaries Inside an Extinct Vent Chimney |
title_short | Copper-Nanocoated Ultra-Small Cells in Grain Boundaries Inside an Extinct Vent Chimney |
title_sort | copper-nanocoated ultra-small cells in grain boundaries inside an extinct vent chimney |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209642/ https://www.ncbi.nlm.nih.gov/pubmed/35747369 http://dx.doi.org/10.3389/fmicb.2022.864205 |
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