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The genome sequence of the avian vampire fly (Philornis downsi), an invasive nest parasite of Darwin’s finches in Galápagos

The invasive avian vampire fly (Philornis downsi, Diptera: Muscidae) is considered one of the greatest threats to the endemic avifauna of the Galápagos Islands. The fly larvae parasitize nearly every passerine species, including Darwin’s finches. Most P. downsi research to date has focused on the ef...

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Autores principales: Romine, Melia G, Knutie, Sarah A, Crow, Carly M, Vaziri, Grace J, Chaves, Jaime A, Koop, Jennifer A H, Lamichhaney, Sangeet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210292/
https://www.ncbi.nlm.nih.gov/pubmed/34878103
http://dx.doi.org/10.1093/g3journal/jkab414
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author Romine, Melia G
Knutie, Sarah A
Crow, Carly M
Vaziri, Grace J
Chaves, Jaime A
Koop, Jennifer A H
Lamichhaney, Sangeet
author_facet Romine, Melia G
Knutie, Sarah A
Crow, Carly M
Vaziri, Grace J
Chaves, Jaime A
Koop, Jennifer A H
Lamichhaney, Sangeet
author_sort Romine, Melia G
collection PubMed
description The invasive avian vampire fly (Philornis downsi, Diptera: Muscidae) is considered one of the greatest threats to the endemic avifauna of the Galápagos Islands. The fly larvae parasitize nearly every passerine species, including Darwin’s finches. Most P. downsi research to date has focused on the effects of the fly on avian host fitness and mitigation methods. A lag in research related to the genetics of this invasion demonstrates, in part, the need to develop full-scale genomic resources with which to address further questions within this system. In this study, an adult female P. downsi was sequenced to generate a high-quality genome assembly. We examined various features of the genome (e.g., coding regions and noncoding transposable elements) and carried out comparative genomics analysis against other dipteran genomes. We identified lists of gene families that are significantly expanding or contracting in P. downsi that are related to insecticide resistance, detoxification, and counter defense against host immune responses. The P. downsi genome assembly provides an important resource for studying the molecular basis of successful invasion in the Galápagos and the dynamics of its population across multiple islands. The findings of significantly changing gene families associated with insecticide resistance and immune responses highlight the need for further investigations into the role of different gene families in aiding the fly’s successful invasion. Furthermore, this genomic resource provides a necessary tool to better inform future research studies and mitigation strategies aimed at minimizing the fly’s impact on Galápagos birds.
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spelling pubmed-92102922022-06-21 The genome sequence of the avian vampire fly (Philornis downsi), an invasive nest parasite of Darwin’s finches in Galápagos Romine, Melia G Knutie, Sarah A Crow, Carly M Vaziri, Grace J Chaves, Jaime A Koop, Jennifer A H Lamichhaney, Sangeet G3 (Bethesda) Genome Report The invasive avian vampire fly (Philornis downsi, Diptera: Muscidae) is considered one of the greatest threats to the endemic avifauna of the Galápagos Islands. The fly larvae parasitize nearly every passerine species, including Darwin’s finches. Most P. downsi research to date has focused on the effects of the fly on avian host fitness and mitigation methods. A lag in research related to the genetics of this invasion demonstrates, in part, the need to develop full-scale genomic resources with which to address further questions within this system. In this study, an adult female P. downsi was sequenced to generate a high-quality genome assembly. We examined various features of the genome (e.g., coding regions and noncoding transposable elements) and carried out comparative genomics analysis against other dipteran genomes. We identified lists of gene families that are significantly expanding or contracting in P. downsi that are related to insecticide resistance, detoxification, and counter defense against host immune responses. The P. downsi genome assembly provides an important resource for studying the molecular basis of successful invasion in the Galápagos and the dynamics of its population across multiple islands. The findings of significantly changing gene families associated with insecticide resistance and immune responses highlight the need for further investigations into the role of different gene families in aiding the fly’s successful invasion. Furthermore, this genomic resource provides a necessary tool to better inform future research studies and mitigation strategies aimed at minimizing the fly’s impact on Galápagos birds. Oxford University Press 2021-12-08 /pmc/articles/PMC9210292/ /pubmed/34878103 http://dx.doi.org/10.1093/g3journal/jkab414 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genome Report
Romine, Melia G
Knutie, Sarah A
Crow, Carly M
Vaziri, Grace J
Chaves, Jaime A
Koop, Jennifer A H
Lamichhaney, Sangeet
The genome sequence of the avian vampire fly (Philornis downsi), an invasive nest parasite of Darwin’s finches in Galápagos
title The genome sequence of the avian vampire fly (Philornis downsi), an invasive nest parasite of Darwin’s finches in Galápagos
title_full The genome sequence of the avian vampire fly (Philornis downsi), an invasive nest parasite of Darwin’s finches in Galápagos
title_fullStr The genome sequence of the avian vampire fly (Philornis downsi), an invasive nest parasite of Darwin’s finches in Galápagos
title_full_unstemmed The genome sequence of the avian vampire fly (Philornis downsi), an invasive nest parasite of Darwin’s finches in Galápagos
title_short The genome sequence of the avian vampire fly (Philornis downsi), an invasive nest parasite of Darwin’s finches in Galápagos
title_sort genome sequence of the avian vampire fly (philornis downsi), an invasive nest parasite of darwin’s finches in galápagos
topic Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210292/
https://www.ncbi.nlm.nih.gov/pubmed/34878103
http://dx.doi.org/10.1093/g3journal/jkab414
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