Evaluation of a pneumonia multiplex PCR panel for detection of bacterial respiratory tract pathogens from serial specimens collected from hospitalized COVID-19 patients

We investigated the concordance between the Unyvero Hospitalized Pneumonia (HPN) application and quantitative culture for detection of bacterial pathogens from serial lower respiratory tract (LRT) specimens collected from the same subject. Comparison of results from HPN application and culture was e...

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Autores principales: Tellapragada, Chaitanya, Ydsten, Karin Andersson, Ternhag, Anders, Giske, Christian G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210330/
https://www.ncbi.nlm.nih.gov/pubmed/35727430
http://dx.doi.org/10.1007/s10096-022-04466-9
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author Tellapragada, Chaitanya
Ydsten, Karin Andersson
Ternhag, Anders
Giske, Christian G.
author_facet Tellapragada, Chaitanya
Ydsten, Karin Andersson
Ternhag, Anders
Giske, Christian G.
author_sort Tellapragada, Chaitanya
collection PubMed
description We investigated the concordance between the Unyvero Hospitalized Pneumonia (HPN) application and quantitative culture for detection of bacterial pathogens from serial lower respiratory tract (LRT) specimens collected from the same subject. Comparison of results from HPN application and culture was evaluated using 69 LRT samples from 27 subjects, using two evaluation approaches. False positive detections by the HPN application was 29% (20/69) in Evaluation I vs 10% (7/68) in Evaluation II. Additional pathogens detected by the HPN application could be confirmed in many instances by culture positivity for the same organism from previous or subsequent samples from the same subject. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10096-022-04466-9.
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spelling pubmed-92103302022-06-21 Evaluation of a pneumonia multiplex PCR panel for detection of bacterial respiratory tract pathogens from serial specimens collected from hospitalized COVID-19 patients Tellapragada, Chaitanya Ydsten, Karin Andersson Ternhag, Anders Giske, Christian G. Eur J Clin Microbiol Infect Dis Brief Report We investigated the concordance between the Unyvero Hospitalized Pneumonia (HPN) application and quantitative culture for detection of bacterial pathogens from serial lower respiratory tract (LRT) specimens collected from the same subject. Comparison of results from HPN application and culture was evaluated using 69 LRT samples from 27 subjects, using two evaluation approaches. False positive detections by the HPN application was 29% (20/69) in Evaluation I vs 10% (7/68) in Evaluation II. Additional pathogens detected by the HPN application could be confirmed in many instances by culture positivity for the same organism from previous or subsequent samples from the same subject. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10096-022-04466-9. Springer Berlin Heidelberg 2022-06-21 2022 /pmc/articles/PMC9210330/ /pubmed/35727430 http://dx.doi.org/10.1007/s10096-022-04466-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Report
Tellapragada, Chaitanya
Ydsten, Karin Andersson
Ternhag, Anders
Giske, Christian G.
Evaluation of a pneumonia multiplex PCR panel for detection of bacterial respiratory tract pathogens from serial specimens collected from hospitalized COVID-19 patients
title Evaluation of a pneumonia multiplex PCR panel for detection of bacterial respiratory tract pathogens from serial specimens collected from hospitalized COVID-19 patients
title_full Evaluation of a pneumonia multiplex PCR panel for detection of bacterial respiratory tract pathogens from serial specimens collected from hospitalized COVID-19 patients
title_fullStr Evaluation of a pneumonia multiplex PCR panel for detection of bacterial respiratory tract pathogens from serial specimens collected from hospitalized COVID-19 patients
title_full_unstemmed Evaluation of a pneumonia multiplex PCR panel for detection of bacterial respiratory tract pathogens from serial specimens collected from hospitalized COVID-19 patients
title_short Evaluation of a pneumonia multiplex PCR panel for detection of bacterial respiratory tract pathogens from serial specimens collected from hospitalized COVID-19 patients
title_sort evaluation of a pneumonia multiplex pcr panel for detection of bacterial respiratory tract pathogens from serial specimens collected from hospitalized covid-19 patients
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210330/
https://www.ncbi.nlm.nih.gov/pubmed/35727430
http://dx.doi.org/10.1007/s10096-022-04466-9
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