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Effects of amyloid-β on protein SUMOylation and levels of mitochondrial proteins in primary cortical neurons
Defining the molecular changes that underlie Alzheimer’s disease (AD) is an important question in neuroscience. Here, we examined changes in protein SUMOylation, and proteins involved in mitochondrial dynamics, in an in vitro model of AD induced by application of amyloid-β 1–42 (Aβ(1–42)) to culture...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210492/ https://www.ncbi.nlm.nih.gov/pubmed/35746977 http://dx.doi.org/10.1016/j.ibneur.2022.01.003 |
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author | Soares, Ericks S. de Souza, Ana C. Guerra Zanella, Camila A. Carmichael, Ruth E. Henley, Jeremy M. Wilkinson, Kevin A. Cimarosti, Helena I. |
author_facet | Soares, Ericks S. de Souza, Ana C. Guerra Zanella, Camila A. Carmichael, Ruth E. Henley, Jeremy M. Wilkinson, Kevin A. Cimarosti, Helena I. |
author_sort | Soares, Ericks S. |
collection | PubMed |
description | Defining the molecular changes that underlie Alzheimer’s disease (AD) is an important question in neuroscience. Here, we examined changes in protein SUMOylation, and proteins involved in mitochondrial dynamics, in an in vitro model of AD induced by application of amyloid-β 1–42 (Aβ(1–42)) to cultured neurons. We observed Aβ(1–42)-induced decreases in global SUMOylation and in levels of the SUMO pathway enzymes SENP3, PIAS1/2, and SAE2. Aβ exposure also decreased levels of the mitochondrial fission proteins Drp1 and Mff and increased activation of caspase-3. To examine whether loss of SENP3 is cytoprotective we knocked down SENP3, which partially prevented the Aβ(1–42)-induced increase in caspase-3 activation. Together, these data support the hypothesis that altered SUMOylation may play a role in the mechanisms underlying AD. |
format | Online Article Text |
id | pubmed-9210492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-92104922022-06-22 Effects of amyloid-β on protein SUMOylation and levels of mitochondrial proteins in primary cortical neurons Soares, Ericks S. de Souza, Ana C. Guerra Zanella, Camila A. Carmichael, Ruth E. Henley, Jeremy M. Wilkinson, Kevin A. Cimarosti, Helena I. IBRO Neurosci Rep Articles from the Latin America Mini Series Defining the molecular changes that underlie Alzheimer’s disease (AD) is an important question in neuroscience. Here, we examined changes in protein SUMOylation, and proteins involved in mitochondrial dynamics, in an in vitro model of AD induced by application of amyloid-β 1–42 (Aβ(1–42)) to cultured neurons. We observed Aβ(1–42)-induced decreases in global SUMOylation and in levels of the SUMO pathway enzymes SENP3, PIAS1/2, and SAE2. Aβ exposure also decreased levels of the mitochondrial fission proteins Drp1 and Mff and increased activation of caspase-3. To examine whether loss of SENP3 is cytoprotective we knocked down SENP3, which partially prevented the Aβ(1–42)-induced increase in caspase-3 activation. Together, these data support the hypothesis that altered SUMOylation may play a role in the mechanisms underlying AD. Elsevier 2022-01-22 /pmc/articles/PMC9210492/ /pubmed/35746977 http://dx.doi.org/10.1016/j.ibneur.2022.01.003 Text en © 2022 The Authors. Published by Elsevier Ltd on behalf of International Brain Research Organization. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Articles from the Latin America Mini Series Soares, Ericks S. de Souza, Ana C. Guerra Zanella, Camila A. Carmichael, Ruth E. Henley, Jeremy M. Wilkinson, Kevin A. Cimarosti, Helena I. Effects of amyloid-β on protein SUMOylation and levels of mitochondrial proteins in primary cortical neurons |
title | Effects of amyloid-β on protein SUMOylation and levels of mitochondrial proteins in primary cortical neurons |
title_full | Effects of amyloid-β on protein SUMOylation and levels of mitochondrial proteins in primary cortical neurons |
title_fullStr | Effects of amyloid-β on protein SUMOylation and levels of mitochondrial proteins in primary cortical neurons |
title_full_unstemmed | Effects of amyloid-β on protein SUMOylation and levels of mitochondrial proteins in primary cortical neurons |
title_short | Effects of amyloid-β on protein SUMOylation and levels of mitochondrial proteins in primary cortical neurons |
title_sort | effects of amyloid-β on protein sumoylation and levels of mitochondrial proteins in primary cortical neurons |
topic | Articles from the Latin America Mini Series |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210492/ https://www.ncbi.nlm.nih.gov/pubmed/35746977 http://dx.doi.org/10.1016/j.ibneur.2022.01.003 |
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