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Investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach
BACKGROUND AND AIM: Specific tumor biomarkers are useful for the early diagnosis of cancer or can predict the recurrence of neoplastic disease in humans and animals. Lymphoma in dogs could be classified into B-, T-, and NK-cell origins. T-cell lymphoma has the worst prognosis with a shorter survival...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210836/ https://www.ncbi.nlm.nih.gov/pubmed/35765478 http://dx.doi.org/10.14202/vetworld.2022.1333-1340 |
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author | Fonghem, Piyanoot Pisitkun, Trairak Rattanapinyopituk, Kasem Sirivisoot, Sirintra Rungsipipat, Anudep |
author_facet | Fonghem, Piyanoot Pisitkun, Trairak Rattanapinyopituk, Kasem Sirivisoot, Sirintra Rungsipipat, Anudep |
author_sort | Fonghem, Piyanoot |
collection | PubMed |
description | BACKGROUND AND AIM: Specific tumor biomarkers are useful for the early diagnosis of cancer or can predict the recurrence of neoplastic disease in humans and animals. Lymphoma in dogs could be classified into B-, T-, and NK-cell origins. T-cell lymphoma has the worst prognosis with a shorter survival time and disease-free interval. This study aimed to identify the differential serum protein expressions of canine B- and T-cell lymphomas compared with healthy dogs using a tandem mass tag (TMT)-based quantitative proteomics. MATERIALS AND METHODS: Serum samples were collected from 20 untreated canine lymphomas (14 B-cells and 6 T-cells) and four healthy control dogs. Sera peptides from each sample were processed for TMT 10-plex tagging and analyzed using liquid chromatography-mass spectrometry (MS). Differential proteome profiling was then compared between lymphoma and control. RESULTS: We discovered 20 elevated and 14 decreased serum proteins in the lymphoma group relative to the healthy group. Six candidate increased proteins in canine lymphomas were beta-actin cytoplasmic 1 (ACTB, p=0.04), haptoglobin (p=0.002), beta-2 microglobulin (aaaaaaaa2M, p=0.007), beta-2 glycoprotein 1 (APOH, p=0.03), metalloproteinase inhibitor 1 (TIMP-1, p=0.03), and CD44 antigen (p=0.02). When compared between B- and T-cell lymphomas, B-cell phenotypes had upregulated immunoglobulin (Ig) heavy chain V region GOM (p=0.02), clusterin (p=0.01), apolipoprotein C1 (APOC1, p=0.05), and plasminogen (p=0.02). CONCLUSION: These findings were investigated quantitative serum proteomes between B- and T-cell lymphomas using TMT-based MS. ACTB, aaaaaaaa2M, APOH, TIMP-1, CD44 antigen, Ig heavy chain V region GOM, and APOC1 are novel candidate proteins and might serve as a lymphoma biomarker in dogs. However, evaluation with an increased sample size is needed to confirm their diagnostic and prognostic ability. |
format | Online Article Text |
id | pubmed-9210836 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-92108362022-06-27 Investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach Fonghem, Piyanoot Pisitkun, Trairak Rattanapinyopituk, Kasem Sirivisoot, Sirintra Rungsipipat, Anudep Vet World Research Article BACKGROUND AND AIM: Specific tumor biomarkers are useful for the early diagnosis of cancer or can predict the recurrence of neoplastic disease in humans and animals. Lymphoma in dogs could be classified into B-, T-, and NK-cell origins. T-cell lymphoma has the worst prognosis with a shorter survival time and disease-free interval. This study aimed to identify the differential serum protein expressions of canine B- and T-cell lymphomas compared with healthy dogs using a tandem mass tag (TMT)-based quantitative proteomics. MATERIALS AND METHODS: Serum samples were collected from 20 untreated canine lymphomas (14 B-cells and 6 T-cells) and four healthy control dogs. Sera peptides from each sample were processed for TMT 10-plex tagging and analyzed using liquid chromatography-mass spectrometry (MS). Differential proteome profiling was then compared between lymphoma and control. RESULTS: We discovered 20 elevated and 14 decreased serum proteins in the lymphoma group relative to the healthy group. Six candidate increased proteins in canine lymphomas were beta-actin cytoplasmic 1 (ACTB, p=0.04), haptoglobin (p=0.002), beta-2 microglobulin (aaaaaaaa2M, p=0.007), beta-2 glycoprotein 1 (APOH, p=0.03), metalloproteinase inhibitor 1 (TIMP-1, p=0.03), and CD44 antigen (p=0.02). When compared between B- and T-cell lymphomas, B-cell phenotypes had upregulated immunoglobulin (Ig) heavy chain V region GOM (p=0.02), clusterin (p=0.01), apolipoprotein C1 (APOC1, p=0.05), and plasminogen (p=0.02). CONCLUSION: These findings were investigated quantitative serum proteomes between B- and T-cell lymphomas using TMT-based MS. ACTB, aaaaaaaa2M, APOH, TIMP-1, CD44 antigen, Ig heavy chain V region GOM, and APOC1 are novel candidate proteins and might serve as a lymphoma biomarker in dogs. However, evaluation with an increased sample size is needed to confirm their diagnostic and prognostic ability. Veterinary World 2022-05 2022-05-26 /pmc/articles/PMC9210836/ /pubmed/35765478 http://dx.doi.org/10.14202/vetworld.2022.1333-1340 Text en Copyright: © Fonghem, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Fonghem, Piyanoot Pisitkun, Trairak Rattanapinyopituk, Kasem Sirivisoot, Sirintra Rungsipipat, Anudep Investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach |
title | Investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach |
title_full | Investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach |
title_fullStr | Investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach |
title_full_unstemmed | Investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach |
title_short | Investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach |
title_sort | investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210836/ https://www.ncbi.nlm.nih.gov/pubmed/35765478 http://dx.doi.org/10.14202/vetworld.2022.1333-1340 |
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