Cargando…

A Combinatorial Vaccine Containing Inactivated Bacterin and Subunits Provides Protection Against Actinobacillus pleuropneumoniae Infection in Mice and Pigs

Actinobacillus pleuropneumoniae (APP) is the etiological agent of porcine contagious pleuropneumonia (PCP) that causes great economic losses in the swine industry. Currently, vaccination is still a commonly used strategy for the prevention of the disease. Commercially available vaccines of this dise...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Lijun, Luo, Wentao, Xiong, Ruyue, Li, Haotian, Yao, Zhiming, Zhuo, Wenxiao, Zou, Geng, Huang, Qi, Zhou, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9212066/
https://www.ncbi.nlm.nih.gov/pubmed/35747235
http://dx.doi.org/10.3389/fvets.2022.902497
_version_ 1784730500784455680
author Zhang, Lijun
Luo, Wentao
Xiong, Ruyue
Li, Haotian
Yao, Zhiming
Zhuo, Wenxiao
Zou, Geng
Huang, Qi
Zhou, Rui
author_facet Zhang, Lijun
Luo, Wentao
Xiong, Ruyue
Li, Haotian
Yao, Zhiming
Zhuo, Wenxiao
Zou, Geng
Huang, Qi
Zhou, Rui
author_sort Zhang, Lijun
collection PubMed
description Actinobacillus pleuropneumoniae (APP) is the etiological agent of porcine contagious pleuropneumonia (PCP) that causes great economic losses in the swine industry. Currently, vaccination is still a commonly used strategy for the prevention of the disease. Commercially available vaccines of this disease, including inactivated bacterins and subunit vaccines, have clinical limitations such as side effects and low cross-protection. In this study, a combinatorial vaccine (Bac-sub) was developed, which contained inactivated bacterial cells of a serovar 1 strain and three recombinant protoxins (rApxIA, rApxIIA, and rApxIIIA). Its side effects, immune protection, and cross-protection were evaluated and compared with a commercial subunit vaccine and a commercial trivalent bacterin in a mouse infection model. The results revealed that the Bac-sub vaccine showed no obvious side effects, and induced higher levels of Apx toxin-specific IgG, IgG1, and IgG2a than the commercial vaccines after booster. After a challenge with virulent strains of serovars 1, 5, and 7, the Bac-sub vaccine provided greater protection (91.76%, 100%, and 100%, respectively) than commercial vaccines. Much lower lung bacterial loads (LBLs) and milder lung lesions were observed in the Bac-sub-vaccinated mice than in those vaccinated with the other two vaccines. The protective efficacy of the Bac-sub vaccine was further evaluated in pigs, which showed that vaccinated pigs displayed significantly milder clinical symptoms and lung lesions than the unvaccinated pigs after the challenge. Taken together, Bac-sub is a safe and effective vaccine that could provide high protection against A. pleuropneumoniae infection in both mice and pigs.
format Online
Article
Text
id pubmed-9212066
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92120662022-06-22 A Combinatorial Vaccine Containing Inactivated Bacterin and Subunits Provides Protection Against Actinobacillus pleuropneumoniae Infection in Mice and Pigs Zhang, Lijun Luo, Wentao Xiong, Ruyue Li, Haotian Yao, Zhiming Zhuo, Wenxiao Zou, Geng Huang, Qi Zhou, Rui Front Vet Sci Veterinary Science Actinobacillus pleuropneumoniae (APP) is the etiological agent of porcine contagious pleuropneumonia (PCP) that causes great economic losses in the swine industry. Currently, vaccination is still a commonly used strategy for the prevention of the disease. Commercially available vaccines of this disease, including inactivated bacterins and subunit vaccines, have clinical limitations such as side effects and low cross-protection. In this study, a combinatorial vaccine (Bac-sub) was developed, which contained inactivated bacterial cells of a serovar 1 strain and three recombinant protoxins (rApxIA, rApxIIA, and rApxIIIA). Its side effects, immune protection, and cross-protection were evaluated and compared with a commercial subunit vaccine and a commercial trivalent bacterin in a mouse infection model. The results revealed that the Bac-sub vaccine showed no obvious side effects, and induced higher levels of Apx toxin-specific IgG, IgG1, and IgG2a than the commercial vaccines after booster. After a challenge with virulent strains of serovars 1, 5, and 7, the Bac-sub vaccine provided greater protection (91.76%, 100%, and 100%, respectively) than commercial vaccines. Much lower lung bacterial loads (LBLs) and milder lung lesions were observed in the Bac-sub-vaccinated mice than in those vaccinated with the other two vaccines. The protective efficacy of the Bac-sub vaccine was further evaluated in pigs, which showed that vaccinated pigs displayed significantly milder clinical symptoms and lung lesions than the unvaccinated pigs after the challenge. Taken together, Bac-sub is a safe and effective vaccine that could provide high protection against A. pleuropneumoniae infection in both mice and pigs. Frontiers Media S.A. 2022-06-07 /pmc/articles/PMC9212066/ /pubmed/35747235 http://dx.doi.org/10.3389/fvets.2022.902497 Text en Copyright © 2022 Zhang, Luo, Xiong, Li, Yao, Zhuo, Zou, Huang and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Zhang, Lijun
Luo, Wentao
Xiong, Ruyue
Li, Haotian
Yao, Zhiming
Zhuo, Wenxiao
Zou, Geng
Huang, Qi
Zhou, Rui
A Combinatorial Vaccine Containing Inactivated Bacterin and Subunits Provides Protection Against Actinobacillus pleuropneumoniae Infection in Mice and Pigs
title A Combinatorial Vaccine Containing Inactivated Bacterin and Subunits Provides Protection Against Actinobacillus pleuropneumoniae Infection in Mice and Pigs
title_full A Combinatorial Vaccine Containing Inactivated Bacterin and Subunits Provides Protection Against Actinobacillus pleuropneumoniae Infection in Mice and Pigs
title_fullStr A Combinatorial Vaccine Containing Inactivated Bacterin and Subunits Provides Protection Against Actinobacillus pleuropneumoniae Infection in Mice and Pigs
title_full_unstemmed A Combinatorial Vaccine Containing Inactivated Bacterin and Subunits Provides Protection Against Actinobacillus pleuropneumoniae Infection in Mice and Pigs
title_short A Combinatorial Vaccine Containing Inactivated Bacterin and Subunits Provides Protection Against Actinobacillus pleuropneumoniae Infection in Mice and Pigs
title_sort combinatorial vaccine containing inactivated bacterin and subunits provides protection against actinobacillus pleuropneumoniae infection in mice and pigs
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9212066/
https://www.ncbi.nlm.nih.gov/pubmed/35747235
http://dx.doi.org/10.3389/fvets.2022.902497
work_keys_str_mv AT zhanglijun acombinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT luowentao acombinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT xiongruyue acombinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT lihaotian acombinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT yaozhiming acombinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT zhuowenxiao acombinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT zougeng acombinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT huangqi acombinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT zhourui acombinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT zhanglijun combinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT luowentao combinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT xiongruyue combinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT lihaotian combinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT yaozhiming combinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT zhuowenxiao combinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT zougeng combinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT huangqi combinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs
AT zhourui combinatorialvaccinecontaininginactivatedbacterinandsubunitsprovidesprotectionagainstactinobacilluspleuropneumoniaeinfectioninmiceandpigs