Evolution, geographic spreading, and demographic distribution of Enterovirus D68

Worldwide outbreaks of enterovirus D68 (EV-D68) in 2014 and 2016 have caused serious respiratory and neurological disease. We collected samples from several European countries during the 2018 outbreak and determined 53 near full-length genome (‘whole genome’) sequences. These sequences were combined...

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Autores principales: Hodcroft, Emma B., Dyrdak, Robert, Andrés, Cristina, Egli, Adrian, Reist, Josiane, García Martínez de Artola, Diego, Alcoba-Flórez, Julia, Niesters, Hubert G. M., Antón, Andrés, Poelman, Randy, Reynders, Marijke, Wollants, Elke, Neher, Richard A., Albert, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9212145/
https://www.ncbi.nlm.nih.gov/pubmed/35639811
http://dx.doi.org/10.1371/journal.ppat.1010515
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author Hodcroft, Emma B.
Dyrdak, Robert
Andrés, Cristina
Egli, Adrian
Reist, Josiane
García Martínez de Artola, Diego
Alcoba-Flórez, Julia
Niesters, Hubert G. M.
Antón, Andrés
Poelman, Randy
Reynders, Marijke
Wollants, Elke
Neher, Richard A.
Albert, Jan
author_facet Hodcroft, Emma B.
Dyrdak, Robert
Andrés, Cristina
Egli, Adrian
Reist, Josiane
García Martínez de Artola, Diego
Alcoba-Flórez, Julia
Niesters, Hubert G. M.
Antón, Andrés
Poelman, Randy
Reynders, Marijke
Wollants, Elke
Neher, Richard A.
Albert, Jan
author_sort Hodcroft, Emma B.
collection PubMed
description Worldwide outbreaks of enterovirus D68 (EV-D68) in 2014 and 2016 have caused serious respiratory and neurological disease. We collected samples from several European countries during the 2018 outbreak and determined 53 near full-length genome (‘whole genome’) sequences. These sequences were combined with 718 whole genome and 1,987 VP1-gene publicly available sequences. In 2018, circulating strains clustered into multiple subgroups in the B3 and A2 subclades, with different phylogenetic origins. Clusters in subclade B3 emerged from strains circulating primarily in the US and Europe in 2016, though some had deeper roots linking to Asian strains, while clusters in A2 traced back to strains detected in East Asia in 2015-2016. In 2018, all sequences from the USA formed a distinct subgroup, containing only three non-US samples. Alongside the varied origins of seasonal strains, we found that diversification of these variants begins up to 18 months prior to the first diagnostic detection during a EV-D68 season. EV-D68 displays strong signs of continuous antigenic evolution and all 2018 A2 strains had novel patterns in the putative neutralizing epitopes in the BC- and DE-loops. The pattern in the BC-loop of the USA B3 subgroup had not been detected on that continent before. Patients with EV-D68 in subclade A2 were significantly older than patients with a B3 subclade virus. In contrast to other subclades, the age distribution of A2 is distinctly bimodal and was found primarily among children and in the elderly. We hypothesize that EV-D68’s rapid evolution of surface proteins, extensive diversity, and high rate of geographic mixing could be explained by substantial reinfection of adults. Better understanding of evolution and immunity across diverse viral pathogens, including EV-D68 and SARS-CoV-2, is critical to pandemic preparedness in the future.
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spelling pubmed-92121452022-06-22 Evolution, geographic spreading, and demographic distribution of Enterovirus D68 Hodcroft, Emma B. Dyrdak, Robert Andrés, Cristina Egli, Adrian Reist, Josiane García Martínez de Artola, Diego Alcoba-Flórez, Julia Niesters, Hubert G. M. Antón, Andrés Poelman, Randy Reynders, Marijke Wollants, Elke Neher, Richard A. Albert, Jan PLoS Pathog Research Article Worldwide outbreaks of enterovirus D68 (EV-D68) in 2014 and 2016 have caused serious respiratory and neurological disease. We collected samples from several European countries during the 2018 outbreak and determined 53 near full-length genome (‘whole genome’) sequences. These sequences were combined with 718 whole genome and 1,987 VP1-gene publicly available sequences. In 2018, circulating strains clustered into multiple subgroups in the B3 and A2 subclades, with different phylogenetic origins. Clusters in subclade B3 emerged from strains circulating primarily in the US and Europe in 2016, though some had deeper roots linking to Asian strains, while clusters in A2 traced back to strains detected in East Asia in 2015-2016. In 2018, all sequences from the USA formed a distinct subgroup, containing only three non-US samples. Alongside the varied origins of seasonal strains, we found that diversification of these variants begins up to 18 months prior to the first diagnostic detection during a EV-D68 season. EV-D68 displays strong signs of continuous antigenic evolution and all 2018 A2 strains had novel patterns in the putative neutralizing epitopes in the BC- and DE-loops. The pattern in the BC-loop of the USA B3 subgroup had not been detected on that continent before. Patients with EV-D68 in subclade A2 were significantly older than patients with a B3 subclade virus. In contrast to other subclades, the age distribution of A2 is distinctly bimodal and was found primarily among children and in the elderly. We hypothesize that EV-D68’s rapid evolution of surface proteins, extensive diversity, and high rate of geographic mixing could be explained by substantial reinfection of adults. Better understanding of evolution and immunity across diverse viral pathogens, including EV-D68 and SARS-CoV-2, is critical to pandemic preparedness in the future. Public Library of Science 2022-05-31 /pmc/articles/PMC9212145/ /pubmed/35639811 http://dx.doi.org/10.1371/journal.ppat.1010515 Text en © 2022 Hodcroft et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hodcroft, Emma B.
Dyrdak, Robert
Andrés, Cristina
Egli, Adrian
Reist, Josiane
García Martínez de Artola, Diego
Alcoba-Flórez, Julia
Niesters, Hubert G. M.
Antón, Andrés
Poelman, Randy
Reynders, Marijke
Wollants, Elke
Neher, Richard A.
Albert, Jan
Evolution, geographic spreading, and demographic distribution of Enterovirus D68
title Evolution, geographic spreading, and demographic distribution of Enterovirus D68
title_full Evolution, geographic spreading, and demographic distribution of Enterovirus D68
title_fullStr Evolution, geographic spreading, and demographic distribution of Enterovirus D68
title_full_unstemmed Evolution, geographic spreading, and demographic distribution of Enterovirus D68
title_short Evolution, geographic spreading, and demographic distribution of Enterovirus D68
title_sort evolution, geographic spreading, and demographic distribution of enterovirus d68
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9212145/
https://www.ncbi.nlm.nih.gov/pubmed/35639811
http://dx.doi.org/10.1371/journal.ppat.1010515
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