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Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus
To seek a new delivery system of vaccine for infectious bronchitis virus (IBV), transgenic potato expressing full-length spike (S) protein of IBV was produced and its immunogenicity in chickens was investigated. One to three copies of S gene of IBV were randomly and stably inserted into potato (Sola...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9212530/ https://www.ncbi.nlm.nih.gov/pubmed/15219399 http://dx.doi.org/10.1016/j.jbiotec.2004.03.012 |
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author | Zhou, Ji-Yong Cheng, Li-Qin Zheng, Xiao-Juan Wu, Jian-Xiang Shang, Shao-Bin Wang, Jin-Yong Chen, Ji-Gang |
author_facet | Zhou, Ji-Yong Cheng, Li-Qin Zheng, Xiao-Juan Wu, Jian-Xiang Shang, Shao-Bin Wang, Jin-Yong Chen, Ji-Gang |
author_sort | Zhou, Ji-Yong |
collection | PubMed |
description | To seek a new delivery system of vaccine for infectious bronchitis virus (IBV), transgenic potato expressing full-length spike (S) protein of IBV was produced and its immunogenicity in chickens was investigated. One to three copies of S gene of IBV were randomly and stably inserted into potato (Solanum tuberrosum cv. Dongnong 303) genome by Agrobacterium tumefaciens-mediated transformation. Transcription and translation of S gene for IBV were confirmed by Northern blot and Western blot analyses in transgenic plantlets. Chickens immunized orally and intramuscularly with transgenic potato tubers expressing S protein generated the detectable levels of serum neutralizing antibodies and were protected against the challenge with the virulent IBV. In vitro secretion of interleukin 2 and T lymphocyte proliferation of spleen cells from the immunized chickens varied with the dose and the manner of vaccination with S protein derived from transgenic plants. The results indicated that S protein expressed in transgenic plants might be a new source for the production of Coronaviridae IBV vaccine. |
format | Online Article Text |
id | pubmed-9212530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92125302022-06-22 Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus Zhou, Ji-Yong Cheng, Li-Qin Zheng, Xiao-Juan Wu, Jian-Xiang Shang, Shao-Bin Wang, Jin-Yong Chen, Ji-Gang J Biotechnol Article To seek a new delivery system of vaccine for infectious bronchitis virus (IBV), transgenic potato expressing full-length spike (S) protein of IBV was produced and its immunogenicity in chickens was investigated. One to three copies of S gene of IBV were randomly and stably inserted into potato (Solanum tuberrosum cv. Dongnong 303) genome by Agrobacterium tumefaciens-mediated transformation. Transcription and translation of S gene for IBV were confirmed by Northern blot and Western blot analyses in transgenic plantlets. Chickens immunized orally and intramuscularly with transgenic potato tubers expressing S protein generated the detectable levels of serum neutralizing antibodies and were protected against the challenge with the virulent IBV. In vitro secretion of interleukin 2 and T lymphocyte proliferation of spleen cells from the immunized chickens varied with the dose and the manner of vaccination with S protein derived from transgenic plants. The results indicated that S protein expressed in transgenic plants might be a new source for the production of Coronaviridae IBV vaccine. Elsevier B.V. 2004-07-15 2004-06-08 /pmc/articles/PMC9212530/ /pubmed/15219399 http://dx.doi.org/10.1016/j.jbiotec.2004.03.012 Text en Copyright © 2004 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Zhou, Ji-Yong Cheng, Li-Qin Zheng, Xiao-Juan Wu, Jian-Xiang Shang, Shao-Bin Wang, Jin-Yong Chen, Ji-Gang Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus |
title | Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus |
title_full | Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus |
title_fullStr | Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus |
title_full_unstemmed | Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus |
title_short | Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus |
title_sort | generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9212530/ https://www.ncbi.nlm.nih.gov/pubmed/15219399 http://dx.doi.org/10.1016/j.jbiotec.2004.03.012 |
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