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Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures for sensitive and selective SARS-CoV-2 sensing

The development of DNA-sensing platforms based on new synthetized Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures (AuNs), as a new pathway to develop a selective and sensitive methodology for SARS-CoV-2 detection is presented. A mixture of gold nanopar...

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Autores principales: Pina-Coronado, Clara, Martínez-Sobrino, Álvaro, Gutiérrez-Gálvez, Laura, Del Caño, Rafael, Martínez-Periñán, Emiliano, García-Nieto, Daniel, Rodríguez-Peña, Micaela, Luna, M., Milán-Rois, Paula, Castellanos, Milagros, Abreu, Melanie, Cantón, Rafael, Galán, Juan Carlos, Pineda, Teresa, Pariente, Félix, Somoza, Álvaro, García-Mendiola, Tania, Miranda, Rodolfo, Lorenzo, Encarnación
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9212675/
https://www.ncbi.nlm.nih.gov/pubmed/35755181
http://dx.doi.org/10.1016/j.snb.2022.132217
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author Pina-Coronado, Clara
Martínez-Sobrino, Álvaro
Gutiérrez-Gálvez, Laura
Del Caño, Rafael
Martínez-Periñán, Emiliano
García-Nieto, Daniel
Rodríguez-Peña, Micaela
Luna, M.
Milán-Rois, Paula
Castellanos, Milagros
Abreu, Melanie
Cantón, Rafael
Galán, Juan Carlos
Pineda, Teresa
Pariente, Félix
Somoza, Álvaro
García-Mendiola, Tania
Miranda, Rodolfo
Lorenzo, Encarnación
author_facet Pina-Coronado, Clara
Martínez-Sobrino, Álvaro
Gutiérrez-Gálvez, Laura
Del Caño, Rafael
Martínez-Periñán, Emiliano
García-Nieto, Daniel
Rodríguez-Peña, Micaela
Luna, M.
Milán-Rois, Paula
Castellanos, Milagros
Abreu, Melanie
Cantón, Rafael
Galán, Juan Carlos
Pineda, Teresa
Pariente, Félix
Somoza, Álvaro
García-Mendiola, Tania
Miranda, Rodolfo
Lorenzo, Encarnación
author_sort Pina-Coronado, Clara
collection PubMed
description The development of DNA-sensing platforms based on new synthetized Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures (AuNs), as a new pathway to develop a selective and sensitive methodology for SARS-CoV-2 detection is presented. A mixture of gold nanoparticles and gold nanotriangles have been synthetized to modify disposable electrodes that act as an enhanced nanostructured electrochemical surface for DNA probe immobilization. On the other hand, modified carbon nanodots prepared a la carte to contain Methylene Blue (MB-CDs) are used as electrochemical indicators of the hybridization event. These MB-CDs, due to their structure, are able to interact differently with double and single-stranded DNA molecules. Based on this strategy, target sequences of the SARS-CoV-2 virus have been detected in a straightforward way and rapidly with a detection limit of 2.00 aM. Moreover, this platform allows the detection of the SARS-CoV-2 sequence in the presence of other viruses, and also a single nucleotide polymorphism (SNPs). The developed approach has been tested directly on RNA obtained from nasopharyngeal samples from COVID-19 patients, avoiding any amplification process. The results agree well with those obtained by RT-qPCR or reverse transcription quantitative polymerase chain reaction technique.
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spelling pubmed-92126752022-06-22 Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures for sensitive and selective SARS-CoV-2 sensing Pina-Coronado, Clara Martínez-Sobrino, Álvaro Gutiérrez-Gálvez, Laura Del Caño, Rafael Martínez-Periñán, Emiliano García-Nieto, Daniel Rodríguez-Peña, Micaela Luna, M. Milán-Rois, Paula Castellanos, Milagros Abreu, Melanie Cantón, Rafael Galán, Juan Carlos Pineda, Teresa Pariente, Félix Somoza, Álvaro García-Mendiola, Tania Miranda, Rodolfo Lorenzo, Encarnación Sens Actuators B Chem Article The development of DNA-sensing platforms based on new synthetized Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures (AuNs), as a new pathway to develop a selective and sensitive methodology for SARS-CoV-2 detection is presented. A mixture of gold nanoparticles and gold nanotriangles have been synthetized to modify disposable electrodes that act as an enhanced nanostructured electrochemical surface for DNA probe immobilization. On the other hand, modified carbon nanodots prepared a la carte to contain Methylene Blue (MB-CDs) are used as electrochemical indicators of the hybridization event. These MB-CDs, due to their structure, are able to interact differently with double and single-stranded DNA molecules. Based on this strategy, target sequences of the SARS-CoV-2 virus have been detected in a straightforward way and rapidly with a detection limit of 2.00 aM. Moreover, this platform allows the detection of the SARS-CoV-2 sequence in the presence of other viruses, and also a single nucleotide polymorphism (SNPs). The developed approach has been tested directly on RNA obtained from nasopharyngeal samples from COVID-19 patients, avoiding any amplification process. The results agree well with those obtained by RT-qPCR or reverse transcription quantitative polymerase chain reaction technique. Elsevier B.V. 2022-10-15 2022-06-17 /pmc/articles/PMC9212675/ /pubmed/35755181 http://dx.doi.org/10.1016/j.snb.2022.132217 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Pina-Coronado, Clara
Martínez-Sobrino, Álvaro
Gutiérrez-Gálvez, Laura
Del Caño, Rafael
Martínez-Periñán, Emiliano
García-Nieto, Daniel
Rodríguez-Peña, Micaela
Luna, M.
Milán-Rois, Paula
Castellanos, Milagros
Abreu, Melanie
Cantón, Rafael
Galán, Juan Carlos
Pineda, Teresa
Pariente, Félix
Somoza, Álvaro
García-Mendiola, Tania
Miranda, Rodolfo
Lorenzo, Encarnación
Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures for sensitive and selective SARS-CoV-2 sensing
title Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures for sensitive and selective SARS-CoV-2 sensing
title_full Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures for sensitive and selective SARS-CoV-2 sensing
title_fullStr Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures for sensitive and selective SARS-CoV-2 sensing
title_full_unstemmed Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures for sensitive and selective SARS-CoV-2 sensing
title_short Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures for sensitive and selective SARS-CoV-2 sensing
title_sort methylene blue functionalized carbon nanodots combined with different shape gold nanostructures for sensitive and selective sars-cov-2 sensing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9212675/
https://www.ncbi.nlm.nih.gov/pubmed/35755181
http://dx.doi.org/10.1016/j.snb.2022.132217
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