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Pseudomonas aeruginosa polysaccharide Psl supports airway microbial community development

Pseudomonas aeruginosa dominates the complex polymicrobial cystic fibrosis (CF) airway and is a leading cause of death in persons with CF. Oral streptococcal colonization has been associated with stable CF lung function. However, no studies have demonstrated how Streptococcus salivarius, the most ab...

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Autores principales: Stoner, Sara N., Baty, Joshua J., Scoffield, Jessica A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213427/
https://www.ncbi.nlm.nih.gov/pubmed/35338335
http://dx.doi.org/10.1038/s41396-022-01221-y
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author Stoner, Sara N.
Baty, Joshua J.
Scoffield, Jessica A.
author_facet Stoner, Sara N.
Baty, Joshua J.
Scoffield, Jessica A.
author_sort Stoner, Sara N.
collection PubMed
description Pseudomonas aeruginosa dominates the complex polymicrobial cystic fibrosis (CF) airway and is a leading cause of death in persons with CF. Oral streptococcal colonization has been associated with stable CF lung function. However, no studies have demonstrated how Streptococcus salivarius, the most abundant streptococcal species found in individuals with stable CF lung disease, potentially improves lung function or becomes incorporated into the CF airway biofilm. By utilizing a two-species biofilm model to probe interactions between S. salivarius and P. aeruginosa, we discovered that the P. aeruginosa exopolysaccharide Psl promoted S. salivarius biofilm formation. Further, we identified a S. salivarius maltose-binding protein (MalE) that is required for promotion of biofilm formation both in vitro and in a Drosophila melanogaster co-infection model. Finally, we demonstrate that promotion of dual biofilm formation with S. salivarius is common among environmental and clinical P. aeruginosa isolates. Overall, our data supports a model in which S. salivarius uses a sugar-binding protein to interact with P. aeruginosa exopolysaccharide, which may be a strategy by which S. salivarius establishes itself within the CF airway microbial community.
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spelling pubmed-92134272022-06-23 Pseudomonas aeruginosa polysaccharide Psl supports airway microbial community development Stoner, Sara N. Baty, Joshua J. Scoffield, Jessica A. ISME J Article Pseudomonas aeruginosa dominates the complex polymicrobial cystic fibrosis (CF) airway and is a leading cause of death in persons with CF. Oral streptococcal colonization has been associated with stable CF lung function. However, no studies have demonstrated how Streptococcus salivarius, the most abundant streptococcal species found in individuals with stable CF lung disease, potentially improves lung function or becomes incorporated into the CF airway biofilm. By utilizing a two-species biofilm model to probe interactions between S. salivarius and P. aeruginosa, we discovered that the P. aeruginosa exopolysaccharide Psl promoted S. salivarius biofilm formation. Further, we identified a S. salivarius maltose-binding protein (MalE) that is required for promotion of biofilm formation both in vitro and in a Drosophila melanogaster co-infection model. Finally, we demonstrate that promotion of dual biofilm formation with S. salivarius is common among environmental and clinical P. aeruginosa isolates. Overall, our data supports a model in which S. salivarius uses a sugar-binding protein to interact with P. aeruginosa exopolysaccharide, which may be a strategy by which S. salivarius establishes itself within the CF airway microbial community. Nature Publishing Group UK 2022-03-25 2022-07 /pmc/articles/PMC9213427/ /pubmed/35338335 http://dx.doi.org/10.1038/s41396-022-01221-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Stoner, Sara N.
Baty, Joshua J.
Scoffield, Jessica A.
Pseudomonas aeruginosa polysaccharide Psl supports airway microbial community development
title Pseudomonas aeruginosa polysaccharide Psl supports airway microbial community development
title_full Pseudomonas aeruginosa polysaccharide Psl supports airway microbial community development
title_fullStr Pseudomonas aeruginosa polysaccharide Psl supports airway microbial community development
title_full_unstemmed Pseudomonas aeruginosa polysaccharide Psl supports airway microbial community development
title_short Pseudomonas aeruginosa polysaccharide Psl supports airway microbial community development
title_sort pseudomonas aeruginosa polysaccharide psl supports airway microbial community development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213427/
https://www.ncbi.nlm.nih.gov/pubmed/35338335
http://dx.doi.org/10.1038/s41396-022-01221-y
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