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Single nucleotide polymorphism determines constitutive versus inducible type VI secretion in Vibrio cholerae

Vibrio cholerae is a well-studied human pathogen that is also a common inhabitant of marine habitats. In both environments, the bacterium is subject to interbacterial competition. A molecular nanomachine that is often involved in such competitive behavior is the type VI secretion system (T6SS). Inte...

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Autores principales: Drebes Dörr, Natália C., Proutière, Alexis, Jaskólska, Milena, Stutzmann, Sandrine, Bader, Loriane, Blokesch, Melanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213491/
https://www.ncbi.nlm.nih.gov/pubmed/35411099
http://dx.doi.org/10.1038/s41396-022-01234-7
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author Drebes Dörr, Natália C.
Proutière, Alexis
Jaskólska, Milena
Stutzmann, Sandrine
Bader, Loriane
Blokesch, Melanie
author_facet Drebes Dörr, Natália C.
Proutière, Alexis
Jaskólska, Milena
Stutzmann, Sandrine
Bader, Loriane
Blokesch, Melanie
author_sort Drebes Dörr, Natália C.
collection PubMed
description Vibrio cholerae is a well-studied human pathogen that is also a common inhabitant of marine habitats. In both environments, the bacterium is subject to interbacterial competition. A molecular nanomachine that is often involved in such competitive behavior is the type VI secretion system (T6SS). Interestingly and in contrast to non-pandemic or environmental isolates, the T6SS of the O1 El Tor clade of V. cholerae, which is responsible for the ongoing 7th cholera pandemic, is largely silent under standard laboratory culture conditions. Instead, these strains induce their full T6SS capacity only under specific conditions such as growth on chitinous surfaces (signaled through TfoX and QstR) or when the cells encounter low intracellular c-di-GMP levels (TfoY-driven). In this study, we identified a single nucleotide polymorphism (SNP) within an intergenic region of the major T6SS gene cluster of V. cholerae that determines the T6SS status of the cell. We show that SNP conversion is sufficient to induce T6SS production in numerous pandemic strains, while the converse approach renders non-pandemic/environmental V. cholerae strains T6SS-silent. We further demonstrate that SNP-dependent T6SS production occurs independently of the known T6SS regulators TfoX, QstR, and TfoY. Finally, we identify a putative promoter region adjacent to the identified SNP that is required for all forms of T6SS regulation in V. cholerae.
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spelling pubmed-92134912022-06-23 Single nucleotide polymorphism determines constitutive versus inducible type VI secretion in Vibrio cholerae Drebes Dörr, Natália C. Proutière, Alexis Jaskólska, Milena Stutzmann, Sandrine Bader, Loriane Blokesch, Melanie ISME J Brief Communication Vibrio cholerae is a well-studied human pathogen that is also a common inhabitant of marine habitats. In both environments, the bacterium is subject to interbacterial competition. A molecular nanomachine that is often involved in such competitive behavior is the type VI secretion system (T6SS). Interestingly and in contrast to non-pandemic or environmental isolates, the T6SS of the O1 El Tor clade of V. cholerae, which is responsible for the ongoing 7th cholera pandemic, is largely silent under standard laboratory culture conditions. Instead, these strains induce their full T6SS capacity only under specific conditions such as growth on chitinous surfaces (signaled through TfoX and QstR) or when the cells encounter low intracellular c-di-GMP levels (TfoY-driven). In this study, we identified a single nucleotide polymorphism (SNP) within an intergenic region of the major T6SS gene cluster of V. cholerae that determines the T6SS status of the cell. We show that SNP conversion is sufficient to induce T6SS production in numerous pandemic strains, while the converse approach renders non-pandemic/environmental V. cholerae strains T6SS-silent. We further demonstrate that SNP-dependent T6SS production occurs independently of the known T6SS regulators TfoX, QstR, and TfoY. Finally, we identify a putative promoter region adjacent to the identified SNP that is required for all forms of T6SS regulation in V. cholerae. Nature Publishing Group UK 2022-04-11 2022-07 /pmc/articles/PMC9213491/ /pubmed/35411099 http://dx.doi.org/10.1038/s41396-022-01234-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Communication
Drebes Dörr, Natália C.
Proutière, Alexis
Jaskólska, Milena
Stutzmann, Sandrine
Bader, Loriane
Blokesch, Melanie
Single nucleotide polymorphism determines constitutive versus inducible type VI secretion in Vibrio cholerae
title Single nucleotide polymorphism determines constitutive versus inducible type VI secretion in Vibrio cholerae
title_full Single nucleotide polymorphism determines constitutive versus inducible type VI secretion in Vibrio cholerae
title_fullStr Single nucleotide polymorphism determines constitutive versus inducible type VI secretion in Vibrio cholerae
title_full_unstemmed Single nucleotide polymorphism determines constitutive versus inducible type VI secretion in Vibrio cholerae
title_short Single nucleotide polymorphism determines constitutive versus inducible type VI secretion in Vibrio cholerae
title_sort single nucleotide polymorphism determines constitutive versus inducible type vi secretion in vibrio cholerae
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213491/
https://www.ncbi.nlm.nih.gov/pubmed/35411099
http://dx.doi.org/10.1038/s41396-022-01234-7
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