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Visualization of learning-induced synaptic plasticity in output neurons of the Drosophila mushroom body γ-lobe
By learning, through experience, which stimuli coincide with dangers, it is possible to predict outcomes and act pre-emptively to ensure survival. In insects, this process is localized to the mushroom body (MB), the circuitry of which facilitates the coincident detection of sensory stimuli and punis...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213513/ https://www.ncbi.nlm.nih.gov/pubmed/35729203 http://dx.doi.org/10.1038/s41598-022-14413-5 |
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author | Hancock, Clare E. Rostami, Vahid Rachad, El Yazid Deimel, Stephan H. Nawrot, Martin P. Fiala, André |
author_facet | Hancock, Clare E. Rostami, Vahid Rachad, El Yazid Deimel, Stephan H. Nawrot, Martin P. Fiala, André |
author_sort | Hancock, Clare E. |
collection | PubMed |
description | By learning, through experience, which stimuli coincide with dangers, it is possible to predict outcomes and act pre-emptively to ensure survival. In insects, this process is localized to the mushroom body (MB), the circuitry of which facilitates the coincident detection of sensory stimuli and punishing or rewarding cues and, downstream, the execution of appropriate learned behaviors. Here, we focused our attention on the mushroom body output neurons (MBONs) of the γ-lobes that act as downstream synaptic partners of the MB γ-Kenyon cells (KCs) to ask how the output of the MB γ-lobe is shaped by olfactory associative conditioning, distinguishing this from non-associative stimulus exposure effects, and without the influence of downstream modulation. This was achieved by employing a subcellularly localized calcium sensor to specifically monitor activity at MBON postsynaptic sites. Therein, we identified a robust associative modulation within only one MBON postsynaptic compartment (MBON-γ1pedc > α/β), which displayed a suppressed postsynaptic response to an aversively paired odor. While this MBON did not undergo non-associative modulation, the reverse was true across the remainder of the γ-lobe, where general odor-evoked adaptation was observed, but no conditioned odor-specific modulation. In conclusion, associative synaptic plasticity underlying aversive olfactory learning is localized to one distinct synaptic γKC-to-γMBON connection. |
format | Online Article Text |
id | pubmed-9213513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-92135132022-06-23 Visualization of learning-induced synaptic plasticity in output neurons of the Drosophila mushroom body γ-lobe Hancock, Clare E. Rostami, Vahid Rachad, El Yazid Deimel, Stephan H. Nawrot, Martin P. Fiala, André Sci Rep Article By learning, through experience, which stimuli coincide with dangers, it is possible to predict outcomes and act pre-emptively to ensure survival. In insects, this process is localized to the mushroom body (MB), the circuitry of which facilitates the coincident detection of sensory stimuli and punishing or rewarding cues and, downstream, the execution of appropriate learned behaviors. Here, we focused our attention on the mushroom body output neurons (MBONs) of the γ-lobes that act as downstream synaptic partners of the MB γ-Kenyon cells (KCs) to ask how the output of the MB γ-lobe is shaped by olfactory associative conditioning, distinguishing this from non-associative stimulus exposure effects, and without the influence of downstream modulation. This was achieved by employing a subcellularly localized calcium sensor to specifically monitor activity at MBON postsynaptic sites. Therein, we identified a robust associative modulation within only one MBON postsynaptic compartment (MBON-γ1pedc > α/β), which displayed a suppressed postsynaptic response to an aversively paired odor. While this MBON did not undergo non-associative modulation, the reverse was true across the remainder of the γ-lobe, where general odor-evoked adaptation was observed, but no conditioned odor-specific modulation. In conclusion, associative synaptic plasticity underlying aversive olfactory learning is localized to one distinct synaptic γKC-to-γMBON connection. Nature Publishing Group UK 2022-06-21 /pmc/articles/PMC9213513/ /pubmed/35729203 http://dx.doi.org/10.1038/s41598-022-14413-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Hancock, Clare E. Rostami, Vahid Rachad, El Yazid Deimel, Stephan H. Nawrot, Martin P. Fiala, André Visualization of learning-induced synaptic plasticity in output neurons of the Drosophila mushroom body γ-lobe |
title | Visualization of learning-induced synaptic plasticity in output neurons of the Drosophila mushroom body γ-lobe |
title_full | Visualization of learning-induced synaptic plasticity in output neurons of the Drosophila mushroom body γ-lobe |
title_fullStr | Visualization of learning-induced synaptic plasticity in output neurons of the Drosophila mushroom body γ-lobe |
title_full_unstemmed | Visualization of learning-induced synaptic plasticity in output neurons of the Drosophila mushroom body γ-lobe |
title_short | Visualization of learning-induced synaptic plasticity in output neurons of the Drosophila mushroom body γ-lobe |
title_sort | visualization of learning-induced synaptic plasticity in output neurons of the drosophila mushroom body γ-lobe |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213513/ https://www.ncbi.nlm.nih.gov/pubmed/35729203 http://dx.doi.org/10.1038/s41598-022-14413-5 |
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