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Imaging of fluorescent polymer dots in relation to channels and immune cells in the lymphatic system

Polymer dots (Pdots) have been applied to imaging lymph nodes (LNs) and lymphatic vessels (LVs) in living mice and rats. However, the mechanism of absorption, distribution, metabolism, and excretion of Pdots in LNs and LVs is still unclear. Therefore, the relationship between Pdots and immune cells,...

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Autores principales: Zhang, Yufan, Zhang, Juxiang, Li, Xiaowei, Li, Jingru, Lu, Shuting, Li, Yuqiao, Ren, Panting, Zhang, Chunfu, Xiong, Liqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213818/
https://www.ncbi.nlm.nih.gov/pubmed/35757035
http://dx.doi.org/10.1016/j.mtbio.2022.100317
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author Zhang, Yufan
Zhang, Juxiang
Li, Xiaowei
Li, Jingru
Lu, Shuting
Li, Yuqiao
Ren, Panting
Zhang, Chunfu
Xiong, Liqin
author_facet Zhang, Yufan
Zhang, Juxiang
Li, Xiaowei
Li, Jingru
Lu, Shuting
Li, Yuqiao
Ren, Panting
Zhang, Chunfu
Xiong, Liqin
author_sort Zhang, Yufan
collection PubMed
description Polymer dots (Pdots) have been applied to imaging lymph nodes (LNs) and lymphatic vessels (LVs) in living mice and rats. However, the mechanism of absorption, distribution, metabolism, and excretion of Pdots in LNs and LVs is still unclear. Therefore, the relationship between Pdots and immune cells, LVs and collagen fibers in lymphatics was studied by multiple in vivo and ex vivo microscopic imaging methods and detection techniques. Flow cytometry showed that Pdots could be phagocytosed by macrophages and monocytes, and had no relationship with B cells, T cells and dendric cells in LNs. Silver staining, immunofluorescence and two-photon microscope showed that Pdots gathered in collagen fibers and LVs of LNs. Furthermore, immunofluorescence imaging results verified that Pdots were distributed in the extracellular space of collecting LVs endothelial cells. In addition, Pdots in the collecting LVs were basically cleared by leaking into the surrounding tissue or draining LNs after 21 days of injection. During the long-time observation, Pdots also helped monitor the contraction frequency and variation range of LV. Our study lays a foundation on the research of Pdots as the carrier to study lymphatic structure and function in the future.
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spelling pubmed-92138182022-06-23 Imaging of fluorescent polymer dots in relation to channels and immune cells in the lymphatic system Zhang, Yufan Zhang, Juxiang Li, Xiaowei Li, Jingru Lu, Shuting Li, Yuqiao Ren, Panting Zhang, Chunfu Xiong, Liqin Mater Today Bio Full Length Article Polymer dots (Pdots) have been applied to imaging lymph nodes (LNs) and lymphatic vessels (LVs) in living mice and rats. However, the mechanism of absorption, distribution, metabolism, and excretion of Pdots in LNs and LVs is still unclear. Therefore, the relationship between Pdots and immune cells, LVs and collagen fibers in lymphatics was studied by multiple in vivo and ex vivo microscopic imaging methods and detection techniques. Flow cytometry showed that Pdots could be phagocytosed by macrophages and monocytes, and had no relationship with B cells, T cells and dendric cells in LNs. Silver staining, immunofluorescence and two-photon microscope showed that Pdots gathered in collagen fibers and LVs of LNs. Furthermore, immunofluorescence imaging results verified that Pdots were distributed in the extracellular space of collecting LVs endothelial cells. In addition, Pdots in the collecting LVs were basically cleared by leaking into the surrounding tissue or draining LNs after 21 days of injection. During the long-time observation, Pdots also helped monitor the contraction frequency and variation range of LV. Our study lays a foundation on the research of Pdots as the carrier to study lymphatic structure and function in the future. Elsevier 2022-06-12 /pmc/articles/PMC9213818/ /pubmed/35757035 http://dx.doi.org/10.1016/j.mtbio.2022.100317 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Zhang, Yufan
Zhang, Juxiang
Li, Xiaowei
Li, Jingru
Lu, Shuting
Li, Yuqiao
Ren, Panting
Zhang, Chunfu
Xiong, Liqin
Imaging of fluorescent polymer dots in relation to channels and immune cells in the lymphatic system
title Imaging of fluorescent polymer dots in relation to channels and immune cells in the lymphatic system
title_full Imaging of fluorescent polymer dots in relation to channels and immune cells in the lymphatic system
title_fullStr Imaging of fluorescent polymer dots in relation to channels and immune cells in the lymphatic system
title_full_unstemmed Imaging of fluorescent polymer dots in relation to channels and immune cells in the lymphatic system
title_short Imaging of fluorescent polymer dots in relation to channels and immune cells in the lymphatic system
title_sort imaging of fluorescent polymer dots in relation to channels and immune cells in the lymphatic system
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213818/
https://www.ncbi.nlm.nih.gov/pubmed/35757035
http://dx.doi.org/10.1016/j.mtbio.2022.100317
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