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Cucurbitacin B, Purified and Characterized From the Rhizome of Corallocarpus epigaeus Exhibits Anti-Melanoma Potential

The ethnomedicinal plant from the Cucurbitaceae family, Corallocarpus epigaeus, or its bioactive derivatives have been widely utilized in traditional medicine owing to their distinct applications against various human ailments and have lured the interest of ethnobotanists and biochemists. Here, we r...

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Autores principales: Aiswarya, Sreekumar Usha Devi, Vikas, Gowda, Haritha, Nair Hariprasad, Liju, Vijayasteltar Belsamma, Shabna, Anwar, Swetha, Mundanattu, Rayginia, Tennyson Prakash, Keerthana, Chenicheri Kizhakkeveettil, Nath, Lekshmi Raghu, Reshma, Mullan Vellandy, Sundaram, Sankar, Anto, Nikhil Ponnoor, Lankalapalli, Ravi Shankar, Anto, Ruby John, Bava, Smitha Vadakkeveettil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213886/
https://www.ncbi.nlm.nih.gov/pubmed/35756619
http://dx.doi.org/10.3389/fonc.2022.903832
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author Aiswarya, Sreekumar Usha Devi
Vikas, Gowda
Haritha, Nair Hariprasad
Liju, Vijayasteltar Belsamma
Shabna, Anwar
Swetha, Mundanattu
Rayginia, Tennyson Prakash
Keerthana, Chenicheri Kizhakkeveettil
Nath, Lekshmi Raghu
Reshma, Mullan Vellandy
Sundaram, Sankar
Anto, Nikhil Ponnoor
Lankalapalli, Ravi Shankar
Anto, Ruby John
Bava, Smitha Vadakkeveettil
author_facet Aiswarya, Sreekumar Usha Devi
Vikas, Gowda
Haritha, Nair Hariprasad
Liju, Vijayasteltar Belsamma
Shabna, Anwar
Swetha, Mundanattu
Rayginia, Tennyson Prakash
Keerthana, Chenicheri Kizhakkeveettil
Nath, Lekshmi Raghu
Reshma, Mullan Vellandy
Sundaram, Sankar
Anto, Nikhil Ponnoor
Lankalapalli, Ravi Shankar
Anto, Ruby John
Bava, Smitha Vadakkeveettil
author_sort Aiswarya, Sreekumar Usha Devi
collection PubMed
description The ethnomedicinal plant from the Cucurbitaceae family, Corallocarpus epigaeus, or its bioactive derivatives have been widely utilized in traditional medicine owing to their distinct applications against various human ailments and have lured the interest of ethnobotanists and biochemists. Here, we report for the first time, the anti-cancer potential of a bio-active fraction isolated from the dried rhizome of C. epigaeus, and the bioactive principle identified as cucurbitacin B (Cu-B). The purification processes involving the utilization of multiple organic extracts of C. epigaeus rhizome powder, yielded Cu-B from the Ethyl acetate Cytotoxic Fraction (ECF), obtained by the chromatographic separation of the ethyl acetate extract. Amongst the various cancer lines tested, melanoma cells exhibit maximal sensitivity towards the Cu-B-containing ECF fraction. Cu-B induces an apoptotic mode of cell death initiated intrinsically as well as extrinsically in A375 melanoma cells whilst remaining comparatively less toxic to normal skin fibroblasts. In vivo studies involving a NOD-SCID murine model of human melanoma demonstrate the ability of Cu-B to attenuate tumor growth, while being pharmacologically safe in vivo, as assessed in Swiss albino mice. Furthermore, Cu-B inhibits MEK 1/2 as well as the constitutive and EGF-induced ERK 1/2 activation, indicating a definitive involvement of MAPK signal transducers in regulating Cu-B-mediated anti-melanoma activity. Together, our study demonstrates the anti-melanoma potential of C. epigaeus-derived Cu-B, which indicates the Cucurbitaceae succulent as a prospective source for deriving potent and pharmacologically safe anti-cancer compounds.
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spelling pubmed-92138862022-06-23 Cucurbitacin B, Purified and Characterized From the Rhizome of Corallocarpus epigaeus Exhibits Anti-Melanoma Potential Aiswarya, Sreekumar Usha Devi Vikas, Gowda Haritha, Nair Hariprasad Liju, Vijayasteltar Belsamma Shabna, Anwar Swetha, Mundanattu Rayginia, Tennyson Prakash Keerthana, Chenicheri Kizhakkeveettil Nath, Lekshmi Raghu Reshma, Mullan Vellandy Sundaram, Sankar Anto, Nikhil Ponnoor Lankalapalli, Ravi Shankar Anto, Ruby John Bava, Smitha Vadakkeveettil Front Oncol Oncology The ethnomedicinal plant from the Cucurbitaceae family, Corallocarpus epigaeus, or its bioactive derivatives have been widely utilized in traditional medicine owing to their distinct applications against various human ailments and have lured the interest of ethnobotanists and biochemists. Here, we report for the first time, the anti-cancer potential of a bio-active fraction isolated from the dried rhizome of C. epigaeus, and the bioactive principle identified as cucurbitacin B (Cu-B). The purification processes involving the utilization of multiple organic extracts of C. epigaeus rhizome powder, yielded Cu-B from the Ethyl acetate Cytotoxic Fraction (ECF), obtained by the chromatographic separation of the ethyl acetate extract. Amongst the various cancer lines tested, melanoma cells exhibit maximal sensitivity towards the Cu-B-containing ECF fraction. Cu-B induces an apoptotic mode of cell death initiated intrinsically as well as extrinsically in A375 melanoma cells whilst remaining comparatively less toxic to normal skin fibroblasts. In vivo studies involving a NOD-SCID murine model of human melanoma demonstrate the ability of Cu-B to attenuate tumor growth, while being pharmacologically safe in vivo, as assessed in Swiss albino mice. Furthermore, Cu-B inhibits MEK 1/2 as well as the constitutive and EGF-induced ERK 1/2 activation, indicating a definitive involvement of MAPK signal transducers in regulating Cu-B-mediated anti-melanoma activity. Together, our study demonstrates the anti-melanoma potential of C. epigaeus-derived Cu-B, which indicates the Cucurbitaceae succulent as a prospective source for deriving potent and pharmacologically safe anti-cancer compounds. Frontiers Media S.A. 2022-06-08 /pmc/articles/PMC9213886/ /pubmed/35756619 http://dx.doi.org/10.3389/fonc.2022.903832 Text en Copyright © 2022 Aiswarya, Vikas, Haritha, Liju, Shabna, Swetha, Rayginia, Keerthana, Nath, Reshma, Sundaram, Anto, Lankalapalli, Anto and Bava https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Aiswarya, Sreekumar Usha Devi
Vikas, Gowda
Haritha, Nair Hariprasad
Liju, Vijayasteltar Belsamma
Shabna, Anwar
Swetha, Mundanattu
Rayginia, Tennyson Prakash
Keerthana, Chenicheri Kizhakkeveettil
Nath, Lekshmi Raghu
Reshma, Mullan Vellandy
Sundaram, Sankar
Anto, Nikhil Ponnoor
Lankalapalli, Ravi Shankar
Anto, Ruby John
Bava, Smitha Vadakkeveettil
Cucurbitacin B, Purified and Characterized From the Rhizome of Corallocarpus epigaeus Exhibits Anti-Melanoma Potential
title Cucurbitacin B, Purified and Characterized From the Rhizome of Corallocarpus epigaeus Exhibits Anti-Melanoma Potential
title_full Cucurbitacin B, Purified and Characterized From the Rhizome of Corallocarpus epigaeus Exhibits Anti-Melanoma Potential
title_fullStr Cucurbitacin B, Purified and Characterized From the Rhizome of Corallocarpus epigaeus Exhibits Anti-Melanoma Potential
title_full_unstemmed Cucurbitacin B, Purified and Characterized From the Rhizome of Corallocarpus epigaeus Exhibits Anti-Melanoma Potential
title_short Cucurbitacin B, Purified and Characterized From the Rhizome of Corallocarpus epigaeus Exhibits Anti-Melanoma Potential
title_sort cucurbitacin b, purified and characterized from the rhizome of corallocarpus epigaeus exhibits anti-melanoma potential
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213886/
https://www.ncbi.nlm.nih.gov/pubmed/35756619
http://dx.doi.org/10.3389/fonc.2022.903832
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