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Optimization of Biomimetic, Leukocyte-Mimicking Nanovesicles for Drug Delivery Against Colorectal Cancer Using a Design of Experiment Approach

The development of biomimetic nanoparticles (NPs) has revolutionized the concept of nanomedicine by offering a completely new set of biocompatible materials to formulate innovative drug delivery systems capable of imitating the behavior of cells. Specifically, the use of leukocyte-derived membrane p...

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Autores principales: Rampado, Riccardo, Biccari, Andrea, D’Angelo, Edoardo, Collino, Federica, Cricrì, Giulia, Caliceti, Paolo, Giordano, Federica, Taraballi, Francesca, Pucciarelli, Salvatore, Agostini, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214241/
https://www.ncbi.nlm.nih.gov/pubmed/35757799
http://dx.doi.org/10.3389/fbioe.2022.883034
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author Rampado, Riccardo
Biccari, Andrea
D’Angelo, Edoardo
Collino, Federica
Cricrì, Giulia
Caliceti, Paolo
Giordano, Federica
Taraballi, Francesca
Pucciarelli, Salvatore
Agostini, Marco
author_facet Rampado, Riccardo
Biccari, Andrea
D’Angelo, Edoardo
Collino, Federica
Cricrì, Giulia
Caliceti, Paolo
Giordano, Federica
Taraballi, Francesca
Pucciarelli, Salvatore
Agostini, Marco
author_sort Rampado, Riccardo
collection PubMed
description The development of biomimetic nanoparticles (NPs) has revolutionized the concept of nanomedicine by offering a completely new set of biocompatible materials to formulate innovative drug delivery systems capable of imitating the behavior of cells. Specifically, the use of leukocyte-derived membrane proteins to functionalize nanovesicles (leukosomes) can enable their long circulation and target the inflamed endothelium present in many inflammatory pathologies and tumors, making them a promising and versatile drug delivery system. However, these studies did not elucidate the critical experimental parameters involved in leukosomes formulation. In the present study, we approached the preparation of leukosomes using a design of experiment (DoE) method to better understand the influence of experimental parameters on leukosomes features such as size, size distribution, and protein loading. We also validated this formulation technologically and tested its behavior in in vitro colorectal cancer (CRC) models, including CRC patient-derived tumor organoids (PDOs). We demonstrated leukosomes biocompatibility, endothelium adhesion capability, and tumor target in three-dimensional (3D) settings using CRC cell lines. Overall, our study offers a novel conceptual framework for biomimetic NPs using a DoE strategy and consolidates the high therapeutic potential of leukosomes as a viable drug delivery system for anti-inflammatory and antineoplastic applications.
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spelling pubmed-92142412022-06-23 Optimization of Biomimetic, Leukocyte-Mimicking Nanovesicles for Drug Delivery Against Colorectal Cancer Using a Design of Experiment Approach Rampado, Riccardo Biccari, Andrea D’Angelo, Edoardo Collino, Federica Cricrì, Giulia Caliceti, Paolo Giordano, Federica Taraballi, Francesca Pucciarelli, Salvatore Agostini, Marco Front Bioeng Biotechnol Bioengineering and Biotechnology The development of biomimetic nanoparticles (NPs) has revolutionized the concept of nanomedicine by offering a completely new set of biocompatible materials to formulate innovative drug delivery systems capable of imitating the behavior of cells. Specifically, the use of leukocyte-derived membrane proteins to functionalize nanovesicles (leukosomes) can enable their long circulation and target the inflamed endothelium present in many inflammatory pathologies and tumors, making them a promising and versatile drug delivery system. However, these studies did not elucidate the critical experimental parameters involved in leukosomes formulation. In the present study, we approached the preparation of leukosomes using a design of experiment (DoE) method to better understand the influence of experimental parameters on leukosomes features such as size, size distribution, and protein loading. We also validated this formulation technologically and tested its behavior in in vitro colorectal cancer (CRC) models, including CRC patient-derived tumor organoids (PDOs). We demonstrated leukosomes biocompatibility, endothelium adhesion capability, and tumor target in three-dimensional (3D) settings using CRC cell lines. Overall, our study offers a novel conceptual framework for biomimetic NPs using a DoE strategy and consolidates the high therapeutic potential of leukosomes as a viable drug delivery system for anti-inflammatory and antineoplastic applications. Frontiers Media S.A. 2022-06-08 /pmc/articles/PMC9214241/ /pubmed/35757799 http://dx.doi.org/10.3389/fbioe.2022.883034 Text en Copyright © 2022 Rampado, Biccari, D’Angelo, Collino, Cricrì, Caliceti, Giordano, Taraballi, Pucciarelli and Agostini. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Rampado, Riccardo
Biccari, Andrea
D’Angelo, Edoardo
Collino, Federica
Cricrì, Giulia
Caliceti, Paolo
Giordano, Federica
Taraballi, Francesca
Pucciarelli, Salvatore
Agostini, Marco
Optimization of Biomimetic, Leukocyte-Mimicking Nanovesicles for Drug Delivery Against Colorectal Cancer Using a Design of Experiment Approach
title Optimization of Biomimetic, Leukocyte-Mimicking Nanovesicles for Drug Delivery Against Colorectal Cancer Using a Design of Experiment Approach
title_full Optimization of Biomimetic, Leukocyte-Mimicking Nanovesicles for Drug Delivery Against Colorectal Cancer Using a Design of Experiment Approach
title_fullStr Optimization of Biomimetic, Leukocyte-Mimicking Nanovesicles for Drug Delivery Against Colorectal Cancer Using a Design of Experiment Approach
title_full_unstemmed Optimization of Biomimetic, Leukocyte-Mimicking Nanovesicles for Drug Delivery Against Colorectal Cancer Using a Design of Experiment Approach
title_short Optimization of Biomimetic, Leukocyte-Mimicking Nanovesicles for Drug Delivery Against Colorectal Cancer Using a Design of Experiment Approach
title_sort optimization of biomimetic, leukocyte-mimicking nanovesicles for drug delivery against colorectal cancer using a design of experiment approach
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214241/
https://www.ncbi.nlm.nih.gov/pubmed/35757799
http://dx.doi.org/10.3389/fbioe.2022.883034
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