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Duration of dry and humidified incubation of single-step embryo culture medium and oxygen tension during sham culture do not alter medium composition.

Background: The extended embryo culture using single-step medium gained popularity in clinical in vitro fertilisation (IVF). However, there are concerns about the degradation of unstable medium components and their negative effects on the developing embryos. Further, dry-incubation can increase osmo...

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Autores principales: Cheredath, Aswathi, Uppangala, Shubhashree, Asampille, Gitanjali, Lakshmi R., Vani, Joseph, David, Raval, Keyur, Gowda G. A., Nagana, Kalthur, Guruprasad, Adiga, Satish Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214662/
https://www.ncbi.nlm.nih.gov/pubmed/35811802
http://dx.doi.org/10.12688/f1000research.109895.4
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author Cheredath, Aswathi
Uppangala, Shubhashree
Asampille, Gitanjali
Lakshmi R., Vani
Joseph, David
Raval, Keyur
Gowda G. A., Nagana
Kalthur, Guruprasad
Adiga, Satish Kumar
author_facet Cheredath, Aswathi
Uppangala, Shubhashree
Asampille, Gitanjali
Lakshmi R., Vani
Joseph, David
Raval, Keyur
Gowda G. A., Nagana
Kalthur, Guruprasad
Adiga, Satish Kumar
author_sort Cheredath, Aswathi
collection PubMed
description Background: The extended embryo culture using single-step medium gained popularity in clinical in vitro fertilisation (IVF). However, there are concerns about the degradation of unstable medium components and their negative effects on the developing embryos. Further, dry-incubation can increase osmolality, which can in-turn enhance the concentration of constituents of the media and their stability. Hence, this study was conducted to understand the immediate changes in the culture media constituents in relation to clinically comparable situations such as single-step extended embryo culture and use of dry and humidified-incubation in two-different gaseous conditions. Methods: Commercially available single-step medium was sham-cultured in droplets under oil in two different conditions viz. dry (37°C; 6%CO 2; 5%O 2) and humidified (37°C; 6% CO 2; atmospheric O 2) for 0h, 72h, and 120h intervals. Droplets were subjected to the sensitivity-enhanced nuclear magnetic resonance (NMR)-based profiling using 800 MHz NMR equipped with a cryogenically cooled micro-coil (1.7mm) probe. NMR profile of the embryo culture medium between the two groups were comprehensively assessed. Results: A total of ten amino acids and four energy substrates were identified from the culture medium. The medium constituents identified showed a non-significant increase in the dry-incubation group at 72h and then declined at 120h. Humidified incubation had no effects on the level of the identified medium constituents until 120h. No significant differences in the levels of medium constituents identified were observed between the dry and humidified-groups at various time-points tested. Conclusions: A non-significant variation in the levels of medium constituents observed in the dry-incubation of single-step medium most unlikely to influence a clinical outcome. However, the impact of these subtle changes on the (epi)genetic integrity of the embryos in a clinical set-up to be addressed.
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spelling pubmed-92146622022-07-08 Duration of dry and humidified incubation of single-step embryo culture medium and oxygen tension during sham culture do not alter medium composition. Cheredath, Aswathi Uppangala, Shubhashree Asampille, Gitanjali Lakshmi R., Vani Joseph, David Raval, Keyur Gowda G. A., Nagana Kalthur, Guruprasad Adiga, Satish Kumar F1000Res Research Article Background: The extended embryo culture using single-step medium gained popularity in clinical in vitro fertilisation (IVF). However, there are concerns about the degradation of unstable medium components and their negative effects on the developing embryos. Further, dry-incubation can increase osmolality, which can in-turn enhance the concentration of constituents of the media and their stability. Hence, this study was conducted to understand the immediate changes in the culture media constituents in relation to clinically comparable situations such as single-step extended embryo culture and use of dry and humidified-incubation in two-different gaseous conditions. Methods: Commercially available single-step medium was sham-cultured in droplets under oil in two different conditions viz. dry (37°C; 6%CO 2; 5%O 2) and humidified (37°C; 6% CO 2; atmospheric O 2) for 0h, 72h, and 120h intervals. Droplets were subjected to the sensitivity-enhanced nuclear magnetic resonance (NMR)-based profiling using 800 MHz NMR equipped with a cryogenically cooled micro-coil (1.7mm) probe. NMR profile of the embryo culture medium between the two groups were comprehensively assessed. Results: A total of ten amino acids and four energy substrates were identified from the culture medium. The medium constituents identified showed a non-significant increase in the dry-incubation group at 72h and then declined at 120h. Humidified incubation had no effects on the level of the identified medium constituents until 120h. No significant differences in the levels of medium constituents identified were observed between the dry and humidified-groups at various time-points tested. Conclusions: A non-significant variation in the levels of medium constituents observed in the dry-incubation of single-step medium most unlikely to influence a clinical outcome. However, the impact of these subtle changes on the (epi)genetic integrity of the embryos in a clinical set-up to be addressed. F1000 Research Limited 2022-05-30 /pmc/articles/PMC9214662/ /pubmed/35811802 http://dx.doi.org/10.12688/f1000research.109895.4 Text en Copyright: © 2022 Cheredath A et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cheredath, Aswathi
Uppangala, Shubhashree
Asampille, Gitanjali
Lakshmi R., Vani
Joseph, David
Raval, Keyur
Gowda G. A., Nagana
Kalthur, Guruprasad
Adiga, Satish Kumar
Duration of dry and humidified incubation of single-step embryo culture medium and oxygen tension during sham culture do not alter medium composition.
title Duration of dry and humidified incubation of single-step embryo culture medium and oxygen tension during sham culture do not alter medium composition.
title_full Duration of dry and humidified incubation of single-step embryo culture medium and oxygen tension during sham culture do not alter medium composition.
title_fullStr Duration of dry and humidified incubation of single-step embryo culture medium and oxygen tension during sham culture do not alter medium composition.
title_full_unstemmed Duration of dry and humidified incubation of single-step embryo culture medium and oxygen tension during sham culture do not alter medium composition.
title_short Duration of dry and humidified incubation of single-step embryo culture medium and oxygen tension during sham culture do not alter medium composition.
title_sort duration of dry and humidified incubation of single-step embryo culture medium and oxygen tension during sham culture do not alter medium composition.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214662/
https://www.ncbi.nlm.nih.gov/pubmed/35811802
http://dx.doi.org/10.12688/f1000research.109895.4
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