A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells

Insufficient production of erythropoietin (EPO) leads to anaemia. Developing methods for the generation and transplantation of EPO-producing cells would allow scientists to design personalised therapeutic solutions. Here we present a simple and highly reproducible protocol for the generation of neur...

Descripción completa

Detalles Bibliográficos
Autores principales: Lavecchia, Angelo Michele, Buttò, Sara, Xinaris, Christodoulos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214825/
https://www.ncbi.nlm.nih.gov/pubmed/35756347
http://dx.doi.org/10.1016/j.mex.2022.101753
Descripción
Sumario:Insufficient production of erythropoietin (EPO) leads to anaemia. Developing methods for the generation and transplantation of EPO-producing cells would allow scientists to design personalised therapeutic solutions. Here we present a simple and highly reproducible protocol for the generation of neural crest cells (NCCs) that can produce and secrete erythropoiesis-competent EPO in response to hypoxia.

Ejemplares similares