A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells

Insufficient production of erythropoietin (EPO) leads to anaemia. Developing methods for the generation and transplantation of EPO-producing cells would allow scientists to design personalised therapeutic solutions. Here we present a simple and highly reproducible protocol for the generation of neur...

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Detalles Bibliográficos
Autores principales: Lavecchia, Angelo Michele, Buttò, Sara, Xinaris, Christodoulos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214825/
https://www.ncbi.nlm.nih.gov/pubmed/35756347
http://dx.doi.org/10.1016/j.mex.2022.101753
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author Lavecchia, Angelo Michele
Buttò, Sara
Xinaris, Christodoulos
author_facet Lavecchia, Angelo Michele
Buttò, Sara
Xinaris, Christodoulos
author_sort Lavecchia, Angelo Michele
collection PubMed
description Insufficient production of erythropoietin (EPO) leads to anaemia. Developing methods for the generation and transplantation of EPO-producing cells would allow scientists to design personalised therapeutic solutions. Here we present a simple and highly reproducible protocol for the generation of neural crest cells (NCCs) that can produce and secrete erythropoiesis-competent EPO in response to hypoxia.
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spelling pubmed-92148252022-06-23 A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells Lavecchia, Angelo Michele Buttò, Sara Xinaris, Christodoulos MethodsX Protocol Article Insufficient production of erythropoietin (EPO) leads to anaemia. Developing methods for the generation and transplantation of EPO-producing cells would allow scientists to design personalised therapeutic solutions. Here we present a simple and highly reproducible protocol for the generation of neural crest cells (NCCs) that can produce and secrete erythropoiesis-competent EPO in response to hypoxia. Elsevier 2022-06-09 /pmc/articles/PMC9214825/ /pubmed/35756347 http://dx.doi.org/10.1016/j.mex.2022.101753 Text en © 2022 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol Article
Lavecchia, Angelo Michele
Buttò, Sara
Xinaris, Christodoulos
A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells
title A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells
title_full A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells
title_fullStr A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells
title_full_unstemmed A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells
title_short A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells
title_sort protocol for the generation of epo - producing neural crest cells from human induced pluripotent stem cells
topic Protocol Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214825/
https://www.ncbi.nlm.nih.gov/pubmed/35756347
http://dx.doi.org/10.1016/j.mex.2022.101753
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