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Protocol for rapid obtention and fractionation of anaerobic bacterial conditioned media to study calcium signaling in enteroendocrine cells

Gut microbiota influences neurodevelopment, behavior and contributes to neurodegenerative disorders. One possible mechanism is the direct modulation of calcium (Ca(2+)) signaling and protein homeostasis in enteroendocrine cells (EECs), a component of the gut epithelium. Here, we present a protocol t...

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Detalles Bibliográficos
Autores principales:	Amorim Neto, Dionisio Pedro, de Castro Fonseca, Matheus
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214868/ https://www.ncbi.nlm.nih.gov/pubmed/35755129 http://dx.doi.org/10.1016/j.xpro.2022.101486

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