Protocol for morphometric analysis of neurons derived from human pluripotent stem cells

The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of hPSCs-de...

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Detalles Bibliográficos
Autores principales: Mucci, Sofía, Rodriguez-Varela, María Soledad, Isaja, Luciana, Ferriol-Laffouillere, Sofía Luján, Sevlever, Gustavo Emilio, Scassa, María Elida, Romorini., Leonardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214874/
https://www.ncbi.nlm.nih.gov/pubmed/35755127
http://dx.doi.org/10.1016/j.xpro.2022.101487
Descripción
Sumario:The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of hPSCs-derived neurons in two- and three-dimensions in vitro cultures. We detail the analysis of soma area and main and secondary dendrites lengths of GFP-transfected neurons and the measurement of area and perimeter of immunostained neurospheres.

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