Protocol for morphometric analysis of neurons derived from human pluripotent stem cells

The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of hPSCs-de...

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Autores principales: Mucci, Sofía, Rodriguez-Varela, María Soledad, Isaja, Luciana, Ferriol-Laffouillere, Sofía Luján, Sevlever, Gustavo Emilio, Scassa, María Elida, Romorini., Leonardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214874/
https://www.ncbi.nlm.nih.gov/pubmed/35755127
http://dx.doi.org/10.1016/j.xpro.2022.101487
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author Mucci, Sofía
Rodriguez-Varela, María Soledad
Isaja, Luciana
Ferriol-Laffouillere, Sofía Luján
Sevlever, Gustavo Emilio
Scassa, María Elida
Romorini., Leonardo
author_facet Mucci, Sofía
Rodriguez-Varela, María Soledad
Isaja, Luciana
Ferriol-Laffouillere, Sofía Luján
Sevlever, Gustavo Emilio
Scassa, María Elida
Romorini., Leonardo
author_sort Mucci, Sofía
collection PubMed
description The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of hPSCs-derived neurons in two- and three-dimensions in vitro cultures. We detail the analysis of soma area and main and secondary dendrites lengths of GFP-transfected neurons and the measurement of area and perimeter of immunostained neurospheres.
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spelling pubmed-92148742022-06-23 Protocol for morphometric analysis of neurons derived from human pluripotent stem cells Mucci, Sofía Rodriguez-Varela, María Soledad Isaja, Luciana Ferriol-Laffouillere, Sofía Luján Sevlever, Gustavo Emilio Scassa, María Elida Romorini., Leonardo STAR Protoc Protocol The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of hPSCs-derived neurons in two- and three-dimensions in vitro cultures. We detail the analysis of soma area and main and secondary dendrites lengths of GFP-transfected neurons and the measurement of area and perimeter of immunostained neurospheres. Elsevier 2022-06-18 /pmc/articles/PMC9214874/ /pubmed/35755127 http://dx.doi.org/10.1016/j.xpro.2022.101487 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Mucci, Sofía
Rodriguez-Varela, María Soledad
Isaja, Luciana
Ferriol-Laffouillere, Sofía Luján
Sevlever, Gustavo Emilio
Scassa, María Elida
Romorini., Leonardo
Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
title Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
title_full Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
title_fullStr Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
title_full_unstemmed Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
title_short Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
title_sort protocol for morphometric analysis of neurons derived from human pluripotent stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214874/
https://www.ncbi.nlm.nih.gov/pubmed/35755127
http://dx.doi.org/10.1016/j.xpro.2022.101487
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