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FMDV 3A Antagonizes the Effect of ANXA1 to Positively Modulate Viral Replication

The RIG-I-like receptor signaling pathway is crucial for producing type I interferon (IFN-I) against RNA viruses. The present study observed that viral infection increased annexin-A1 (ANXA1) expression, and ANXA1 then promoted RNA virus-induced IFN-I production. Compared to ANXA1 wild-type cells, AN...

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Autores principales: Ma, XuSheng, Zhang, KeShan, Luo, ZhiKuan, Nian, XiaoFeng, Choudhury, S. K. Mohiuddin, Zhu, ZiXiang, Song, Rui, Pei, JingJing, Huo, YanLi, Li, YuanYuan, Yang, Fan, Cao, WeiJun, Liu, HuiSheng, Liu, XiangTao, Zheng, HaiXue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9215249/
https://www.ncbi.nlm.nih.gov/pubmed/35604142
http://dx.doi.org/10.1128/jvi.00317-22
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author Ma, XuSheng
Zhang, KeShan
Luo, ZhiKuan
Nian, XiaoFeng
Choudhury, S. K. Mohiuddin
Zhu, ZiXiang
Song, Rui
Pei, JingJing
Huo, YanLi
Li, YuanYuan
Yang, Fan
Cao, WeiJun
Liu, HuiSheng
Liu, XiangTao
Zheng, HaiXue
author_facet Ma, XuSheng
Zhang, KeShan
Luo, ZhiKuan
Nian, XiaoFeng
Choudhury, S. K. Mohiuddin
Zhu, ZiXiang
Song, Rui
Pei, JingJing
Huo, YanLi
Li, YuanYuan
Yang, Fan
Cao, WeiJun
Liu, HuiSheng
Liu, XiangTao
Zheng, HaiXue
author_sort Ma, XuSheng
collection PubMed
description The RIG-I-like receptor signaling pathway is crucial for producing type I interferon (IFN-I) against RNA viruses. The present study observed that viral infection increased annexin-A1 (ANXA1) expression, and ANXA1 then promoted RNA virus-induced IFN-I production. Compared to ANXA1 wild-type cells, ANXA1(−/−) knockout cells showed IFN-β production decreasing after viral stimulation. RNA virus stimulation induced ANXA1 to regulate IFN-β production through the TBK1-IRF3 axis but not through the NF-κB axis. ANXA1 also interacted with JAK1 and STAT1 to increase signal transduction induced by IFN-β or IFN-γ. We assessed the effect of ANXA1 on the replication of foot-and-mouth disease virus (FMDV) and found that ANXA1 inhibits FMDV replication dependent on IFN-I production. FMDV 3A plays critical roles in viral replication and host range. The results showed that FMDV 3A interacts with ANXA1 to inhibit its ability to promote IFN-β production. We also demonstrated that FMDV 3A inhibits the formation of ANXA1-TBK1 complex. These results indicate that ANXA1 positively regulates RNA virus-stimulated IFN-β production and FMDV 3A antagonizes ANXA1-promoted IFN-β production to modulate viral replication. IMPORTANCE FMDV is a pathogen that causes one of the world’s most destructive and highly contagious animal diseases. The FMDV 3A protein plays a critical role in viral replication and host range. Although 3A is one of the viral proteins that influences FMDV virulence, its underlying mechanisms remain unclear. ANXA1 is involved in immune activation against pathogens. The present study demonstrated that FMDV increases ANXA1 expression, while ANXA1 inhibits FMDV replication. The results also showed that ANXA1 promotes RNA virus-induced IFN-I production through the IRF3 axis at VISA and TBK1 levels. ANXA1 was also found to interact with JAK1 and STAT1 to strengthen signal transduction induced by IFN-β and IFN-γ. 3A interacted with ANXA1 to inhibit ANXA1-TBK1 complex formation, thereby antagonizing the inhibitory effect of ANXA1 on FMDV replication. This study helps to elucidate the mechanism underlying the effect of the 3A protein on FMDV replication.
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spelling pubmed-92152492022-06-23 FMDV 3A Antagonizes the Effect of ANXA1 to Positively Modulate Viral Replication Ma, XuSheng Zhang, KeShan Luo, ZhiKuan Nian, XiaoFeng Choudhury, S. K. Mohiuddin Zhu, ZiXiang Song, Rui Pei, JingJing Huo, YanLi Li, YuanYuan Yang, Fan Cao, WeiJun Liu, HuiSheng Liu, XiangTao Zheng, HaiXue J Virol Pathogenesis and Immunity The RIG-I-like receptor signaling pathway is crucial for producing type I interferon (IFN-I) against RNA viruses. The present study observed that viral infection increased annexin-A1 (ANXA1) expression, and ANXA1 then promoted RNA virus-induced IFN-I production. Compared to ANXA1 wild-type cells, ANXA1(−/−) knockout cells showed IFN-β production decreasing after viral stimulation. RNA virus stimulation induced ANXA1 to regulate IFN-β production through the TBK1-IRF3 axis but not through the NF-κB axis. ANXA1 also interacted with JAK1 and STAT1 to increase signal transduction induced by IFN-β or IFN-γ. We assessed the effect of ANXA1 on the replication of foot-and-mouth disease virus (FMDV) and found that ANXA1 inhibits FMDV replication dependent on IFN-I production. FMDV 3A plays critical roles in viral replication and host range. The results showed that FMDV 3A interacts with ANXA1 to inhibit its ability to promote IFN-β production. We also demonstrated that FMDV 3A inhibits the formation of ANXA1-TBK1 complex. These results indicate that ANXA1 positively regulates RNA virus-stimulated IFN-β production and FMDV 3A antagonizes ANXA1-promoted IFN-β production to modulate viral replication. IMPORTANCE FMDV is a pathogen that causes one of the world’s most destructive and highly contagious animal diseases. The FMDV 3A protein plays a critical role in viral replication and host range. Although 3A is one of the viral proteins that influences FMDV virulence, its underlying mechanisms remain unclear. ANXA1 is involved in immune activation against pathogens. The present study demonstrated that FMDV increases ANXA1 expression, while ANXA1 inhibits FMDV replication. The results also showed that ANXA1 promotes RNA virus-induced IFN-I production through the IRF3 axis at VISA and TBK1 levels. ANXA1 was also found to interact with JAK1 and STAT1 to strengthen signal transduction induced by IFN-β and IFN-γ. 3A interacted with ANXA1 to inhibit ANXA1-TBK1 complex formation, thereby antagonizing the inhibitory effect of ANXA1 on FMDV replication. This study helps to elucidate the mechanism underlying the effect of the 3A protein on FMDV replication. American Society for Microbiology 2022-05-23 /pmc/articles/PMC9215249/ /pubmed/35604142 http://dx.doi.org/10.1128/jvi.00317-22 Text en Copyright © 2022 Ma et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Pathogenesis and Immunity
Ma, XuSheng
Zhang, KeShan
Luo, ZhiKuan
Nian, XiaoFeng
Choudhury, S. K. Mohiuddin
Zhu, ZiXiang
Song, Rui
Pei, JingJing
Huo, YanLi
Li, YuanYuan
Yang, Fan
Cao, WeiJun
Liu, HuiSheng
Liu, XiangTao
Zheng, HaiXue
FMDV 3A Antagonizes the Effect of ANXA1 to Positively Modulate Viral Replication
title FMDV 3A Antagonizes the Effect of ANXA1 to Positively Modulate Viral Replication
title_full FMDV 3A Antagonizes the Effect of ANXA1 to Positively Modulate Viral Replication
title_fullStr FMDV 3A Antagonizes the Effect of ANXA1 to Positively Modulate Viral Replication
title_full_unstemmed FMDV 3A Antagonizes the Effect of ANXA1 to Positively Modulate Viral Replication
title_short FMDV 3A Antagonizes the Effect of ANXA1 to Positively Modulate Viral Replication
title_sort fmdv 3a antagonizes the effect of anxa1 to positively modulate viral replication
topic Pathogenesis and Immunity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9215249/
https://www.ncbi.nlm.nih.gov/pubmed/35604142
http://dx.doi.org/10.1128/jvi.00317-22
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