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Unveiling the Pathogenic Bacteria Causing Descending Necrotizing Mediastinitis
The combination of maxillofacial infections (MI) with descending necrotizing mediastinitis (DNM) is a complex disease characterized by rapid development and high mortality. Here, we performed metagenomic next-generation sequencing (mNGS) using samples from 21 patients with MI and eight patients with...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9215328/ https://www.ncbi.nlm.nih.gov/pubmed/35755831 http://dx.doi.org/10.3389/fcimb.2022.873161 |
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author | Sun, Qiang Li, Zixuan Wang, Panpan Zhao, Junfang Chen, Shuai Sun, Minglei |
author_facet | Sun, Qiang Li, Zixuan Wang, Panpan Zhao, Junfang Chen, Shuai Sun, Minglei |
author_sort | Sun, Qiang |
collection | PubMed |
description | The combination of maxillofacial infections (MI) with descending necrotizing mediastinitis (DNM) is a complex disease characterized by rapid development and high mortality. Here, we performed metagenomic next-generation sequencing (mNGS) using samples from 21 patients with MI and eight patients with DNM. In this study, we found that the species richness of the DNM group was higher than that of the MI group, and the species diversity of the DNM group was higher than that of the MI group, with no statistically significant differences between groups (P > 0.05). LefSE analysis revealed that the main species differing between groups were Bacillus, Lactobacillus, Streptococcaceae, and Streptococcus (S. constellatus and S. anginosus). In addition, the PLS-DA analysis revealed that the dominant groups in the DNM group at the species level were S. constellatus, S. anginosus, Streptococcus intermedius, Prevotella oris, Mogibacterium timidum, and Eubacterium nodatum. Next, we correlated the clinical characteristics of the patients with the relative abundance of the pathogens identified in the LefSe and PLS-DA analyses. The relative abundance of S. anginosus was positively correlated with C-reactive protein (CRP) and calcitoninogen (PCT) but negatively correlated with the percentage of lymphocytes (Lymph%) (P < 0.05). On the other hand, M. timidum was positively correlated with the percentage of neutrophils (Neut%) and glycated hemoglobin (GLU) (P < 0.05), and Parvimonas micra was positively correlated with CRP (P < 0.05). |
format | Online Article Text |
id | pubmed-9215328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92153282022-06-23 Unveiling the Pathogenic Bacteria Causing Descending Necrotizing Mediastinitis Sun, Qiang Li, Zixuan Wang, Panpan Zhao, Junfang Chen, Shuai Sun, Minglei Front Cell Infect Microbiol Cellular and Infection Microbiology The combination of maxillofacial infections (MI) with descending necrotizing mediastinitis (DNM) is a complex disease characterized by rapid development and high mortality. Here, we performed metagenomic next-generation sequencing (mNGS) using samples from 21 patients with MI and eight patients with DNM. In this study, we found that the species richness of the DNM group was higher than that of the MI group, and the species diversity of the DNM group was higher than that of the MI group, with no statistically significant differences between groups (P > 0.05). LefSE analysis revealed that the main species differing between groups were Bacillus, Lactobacillus, Streptococcaceae, and Streptococcus (S. constellatus and S. anginosus). In addition, the PLS-DA analysis revealed that the dominant groups in the DNM group at the species level were S. constellatus, S. anginosus, Streptococcus intermedius, Prevotella oris, Mogibacterium timidum, and Eubacterium nodatum. Next, we correlated the clinical characteristics of the patients with the relative abundance of the pathogens identified in the LefSe and PLS-DA analyses. The relative abundance of S. anginosus was positively correlated with C-reactive protein (CRP) and calcitoninogen (PCT) but negatively correlated with the percentage of lymphocytes (Lymph%) (P < 0.05). On the other hand, M. timidum was positively correlated with the percentage of neutrophils (Neut%) and glycated hemoglobin (GLU) (P < 0.05), and Parvimonas micra was positively correlated with CRP (P < 0.05). Frontiers Media S.A. 2022-06-08 /pmc/articles/PMC9215328/ /pubmed/35755831 http://dx.doi.org/10.3389/fcimb.2022.873161 Text en Copyright © 2022 Sun, Li, Wang, Zhao, Chen and Sun https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Sun, Qiang Li, Zixuan Wang, Panpan Zhao, Junfang Chen, Shuai Sun, Minglei Unveiling the Pathogenic Bacteria Causing Descending Necrotizing Mediastinitis |
title | Unveiling the Pathogenic Bacteria Causing Descending Necrotizing Mediastinitis |
title_full | Unveiling the Pathogenic Bacteria Causing Descending Necrotizing Mediastinitis |
title_fullStr | Unveiling the Pathogenic Bacteria Causing Descending Necrotizing Mediastinitis |
title_full_unstemmed | Unveiling the Pathogenic Bacteria Causing Descending Necrotizing Mediastinitis |
title_short | Unveiling the Pathogenic Bacteria Causing Descending Necrotizing Mediastinitis |
title_sort | unveiling the pathogenic bacteria causing descending necrotizing mediastinitis |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9215328/ https://www.ncbi.nlm.nih.gov/pubmed/35755831 http://dx.doi.org/10.3389/fcimb.2022.873161 |
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