Cargando…

Prevalence of non-tuberculous mycobacteria among previously treated TB patients in the Gulf of Guinea, Africa

OBJECTIVE: Differentiation between non-tuberculous mycobacteria (NTM) and Mycobacterium tuberculosis complex (MTBC) is crucial for case management with the appropriate antimycobacterials. This study was undertaken in three West and Central African countries to understand NTM associated with pulmonar...

Descripción completa

Detalles Bibliográficos
Autores principales: Pokam, B.D. Thumamo, Yeboah-Manu, D., Ofori, S., Guemdjom, P.W., Teyim, P.M., Lawson, L., Amiteye, D., Yhiler, N.Y., Djuikoue, I.C., Asuquo, A.E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9216630/
https://www.ncbi.nlm.nih.gov/pubmed/35755455
http://dx.doi.org/10.1016/j.ijregi.2022.05.003
_version_ 1784731468312870912
author Pokam, B.D. Thumamo
Yeboah-Manu, D.
Ofori, S.
Guemdjom, P.W.
Teyim, P.M.
Lawson, L.
Amiteye, D.
Yhiler, N.Y.
Djuikoue, I.C.
Asuquo, A.E.
author_facet Pokam, B.D. Thumamo
Yeboah-Manu, D.
Ofori, S.
Guemdjom, P.W.
Teyim, P.M.
Lawson, L.
Amiteye, D.
Yhiler, N.Y.
Djuikoue, I.C.
Asuquo, A.E.
author_sort Pokam, B.D. Thumamo
collection PubMed
description OBJECTIVE: Differentiation between non-tuberculous mycobacteria (NTM) and Mycobacterium tuberculosis complex (MTBC) is crucial for case management with the appropriate antimycobacterials. This study was undertaken in three West and Central African countries to understand NTM associated with pulmonary tuberculosis in the sub-region. METHODS: A collection of 503 isolates (158 from Cameroon, 202 from Nigeria and 143 from Ghana) obtained from solid and liquid cultures were analysed. The isolates were tested for drug susceptibility, and MTBC were confirmed using IS6110. All IS6110-negative isolates were identified by 65-kilodalton heat shock protein (hsp65) gene amplification, DNA sequencing and BLAST analysis. RESULTS: Overall, the prevalence of NTM was 16/503 (3.2%), distributed as 2/202 (1%) in Nigeria, 2/158 (1.3%) in Cameroon and 12/143 (8.4%) in Ghana. The main NTM isolates included 5/16 (31.3%) M. fortuitum, 2/16 (12.5%) M. intracellulare and 2/16 (12.5%) M. engbaekii. Eight (57.1%) of the 14 previously treated patients harboured NTM (odds ratio 0.21, 95% confidence interval 0.06–0.77; P=0.021). Three multi-drug-resistant strains were identified: M. engbaekii, M. fortuitum and M. intracellulare. CONCLUSION: NTM were mainly found among individuals with unsuccessful treatment. This highlights the need for mycobacterial species differentiation using rapid molecular tools for appropriate case management, as most are resistant to routine first-line antimycobacterials.
format Online
Article
Text
id pubmed-9216630
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-92166302022-06-24 Prevalence of non-tuberculous mycobacteria among previously treated TB patients in the Gulf of Guinea, Africa Pokam, B.D. Thumamo Yeboah-Manu, D. Ofori, S. Guemdjom, P.W. Teyim, P.M. Lawson, L. Amiteye, D. Yhiler, N.Y. Djuikoue, I.C. Asuquo, A.E. IJID Reg Original Report OBJECTIVE: Differentiation between non-tuberculous mycobacteria (NTM) and Mycobacterium tuberculosis complex (MTBC) is crucial for case management with the appropriate antimycobacterials. This study was undertaken in three West and Central African countries to understand NTM associated with pulmonary tuberculosis in the sub-region. METHODS: A collection of 503 isolates (158 from Cameroon, 202 from Nigeria and 143 from Ghana) obtained from solid and liquid cultures were analysed. The isolates were tested for drug susceptibility, and MTBC were confirmed using IS6110. All IS6110-negative isolates were identified by 65-kilodalton heat shock protein (hsp65) gene amplification, DNA sequencing and BLAST analysis. RESULTS: Overall, the prevalence of NTM was 16/503 (3.2%), distributed as 2/202 (1%) in Nigeria, 2/158 (1.3%) in Cameroon and 12/143 (8.4%) in Ghana. The main NTM isolates included 5/16 (31.3%) M. fortuitum, 2/16 (12.5%) M. intracellulare and 2/16 (12.5%) M. engbaekii. Eight (57.1%) of the 14 previously treated patients harboured NTM (odds ratio 0.21, 95% confidence interval 0.06–0.77; P=0.021). Three multi-drug-resistant strains were identified: M. engbaekii, M. fortuitum and M. intracellulare. CONCLUSION: NTM were mainly found among individuals with unsuccessful treatment. This highlights the need for mycobacterial species differentiation using rapid molecular tools for appropriate case management, as most are resistant to routine first-line antimycobacterials. Elsevier 2022-05-14 /pmc/articles/PMC9216630/ /pubmed/35755455 http://dx.doi.org/10.1016/j.ijregi.2022.05.003 Text en © 2022 Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Report
Pokam, B.D. Thumamo
Yeboah-Manu, D.
Ofori, S.
Guemdjom, P.W.
Teyim, P.M.
Lawson, L.
Amiteye, D.
Yhiler, N.Y.
Djuikoue, I.C.
Asuquo, A.E.
Prevalence of non-tuberculous mycobacteria among previously treated TB patients in the Gulf of Guinea, Africa
title Prevalence of non-tuberculous mycobacteria among previously treated TB patients in the Gulf of Guinea, Africa
title_full Prevalence of non-tuberculous mycobacteria among previously treated TB patients in the Gulf of Guinea, Africa
title_fullStr Prevalence of non-tuberculous mycobacteria among previously treated TB patients in the Gulf of Guinea, Africa
title_full_unstemmed Prevalence of non-tuberculous mycobacteria among previously treated TB patients in the Gulf of Guinea, Africa
title_short Prevalence of non-tuberculous mycobacteria among previously treated TB patients in the Gulf of Guinea, Africa
title_sort prevalence of non-tuberculous mycobacteria among previously treated tb patients in the gulf of guinea, africa
topic Original Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9216630/
https://www.ncbi.nlm.nih.gov/pubmed/35755455
http://dx.doi.org/10.1016/j.ijregi.2022.05.003
work_keys_str_mv AT pokambdthumamo prevalenceofnontuberculousmycobacteriaamongpreviouslytreatedtbpatientsinthegulfofguineaafrica
AT yeboahmanud prevalenceofnontuberculousmycobacteriaamongpreviouslytreatedtbpatientsinthegulfofguineaafrica
AT oforis prevalenceofnontuberculousmycobacteriaamongpreviouslytreatedtbpatientsinthegulfofguineaafrica
AT guemdjompw prevalenceofnontuberculousmycobacteriaamongpreviouslytreatedtbpatientsinthegulfofguineaafrica
AT teyimpm prevalenceofnontuberculousmycobacteriaamongpreviouslytreatedtbpatientsinthegulfofguineaafrica
AT lawsonl prevalenceofnontuberculousmycobacteriaamongpreviouslytreatedtbpatientsinthegulfofguineaafrica
AT amiteyed prevalenceofnontuberculousmycobacteriaamongpreviouslytreatedtbpatientsinthegulfofguineaafrica
AT yhilerny prevalenceofnontuberculousmycobacteriaamongpreviouslytreatedtbpatientsinthegulfofguineaafrica
AT djuikoueic prevalenceofnontuberculousmycobacteriaamongpreviouslytreatedtbpatientsinthegulfofguineaafrica
AT asuquoae prevalenceofnontuberculousmycobacteriaamongpreviouslytreatedtbpatientsinthegulfofguineaafrica