A single-component luminescent biosensor for the SARS-CoV-2 spike protein

Many existing protein detection strategies depend on highly functionalized antibody reagents. A simpler and easier to produce class of detection reagent is highly desirable. We designed a single-component, recombinant, luminescent biosensor that can be expressed in laboratory strains of E. coli and...

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Detalles Bibliográficos
Autores principales: Ravalin, Matthew, Roh, Heegwang, Suryawanshi, Rahul, Kumar, G. Renuka, Pak, John, Ott, Melanie, Ting, Alice Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9216720/
https://www.ncbi.nlm.nih.gov/pubmed/35734091
http://dx.doi.org/10.1101/2022.06.15.496006
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author Ravalin, Matthew
Roh, Heegwang
Suryawanshi, Rahul
Kumar, G. Renuka
Pak, John
Ott, Melanie
Ting, Alice Y.
author_facet Ravalin, Matthew
Roh, Heegwang
Suryawanshi, Rahul
Kumar, G. Renuka
Pak, John
Ott, Melanie
Ting, Alice Y.
author_sort Ravalin, Matthew
collection PubMed
description Many existing protein detection strategies depend on highly functionalized antibody reagents. A simpler and easier to produce class of detection reagent is highly desirable. We designed a single-component, recombinant, luminescent biosensor that can be expressed in laboratory strains of E. coli and S. cerevisiae. This biosensor is deployed in multiple homogenous and immobilized assay formats to detect recombinant SARS-CoV-2 spike antigen and cultured virus. The chemiluminescent signal generated facilitates detection by an un-augmented cell phone camera. Binding Activated Tandem split-enzyme (BAT) biosensors may serve as a useful template for diagnostics and reagents that detect SARS-CoV-2 antigens and other proteins of interest.
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spelling pubmed-92167202022-06-23 A single-component luminescent biosensor for the SARS-CoV-2 spike protein Ravalin, Matthew Roh, Heegwang Suryawanshi, Rahul Kumar, G. Renuka Pak, John Ott, Melanie Ting, Alice Y. bioRxiv Article Many existing protein detection strategies depend on highly functionalized antibody reagents. A simpler and easier to produce class of detection reagent is highly desirable. We designed a single-component, recombinant, luminescent biosensor that can be expressed in laboratory strains of E. coli and S. cerevisiae. This biosensor is deployed in multiple homogenous and immobilized assay formats to detect recombinant SARS-CoV-2 spike antigen and cultured virus. The chemiluminescent signal generated facilitates detection by an un-augmented cell phone camera. Binding Activated Tandem split-enzyme (BAT) biosensors may serve as a useful template for diagnostics and reagents that detect SARS-CoV-2 antigens and other proteins of interest. Cold Spring Harbor Laboratory 2022-06-15 /pmc/articles/PMC9216720/ /pubmed/35734091 http://dx.doi.org/10.1101/2022.06.15.496006 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Ravalin, Matthew
Roh, Heegwang
Suryawanshi, Rahul
Kumar, G. Renuka
Pak, John
Ott, Melanie
Ting, Alice Y.
A single-component luminescent biosensor for the SARS-CoV-2 spike protein
title A single-component luminescent biosensor for the SARS-CoV-2 spike protein
title_full A single-component luminescent biosensor for the SARS-CoV-2 spike protein
title_fullStr A single-component luminescent biosensor for the SARS-CoV-2 spike protein
title_full_unstemmed A single-component luminescent biosensor for the SARS-CoV-2 spike protein
title_short A single-component luminescent biosensor for the SARS-CoV-2 spike protein
title_sort single-component luminescent biosensor for the sars-cov-2 spike protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9216720/
https://www.ncbi.nlm.nih.gov/pubmed/35734091
http://dx.doi.org/10.1101/2022.06.15.496006
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