Cargando…

Emergency SARS-CoV-2 variants of concern: rapidly direct RT-qPCR detection without RNA extraction, clinical comparison, cost-effective, and high-throughput

Since the late 2020, the evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern has been characterized by the emergence of spike protein mutations, and these variants have become dominant worldwide. The gold standard SARS-CoV-2 diagnosis protocol requires two c...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yang, Bing-Heng, Chung, Hsing-Yi, Kao, Li-Ting, Jian, Ming-Jr, Chang, Chih-Kai, Lin, Jung-Chung, Yeh, Kuo-Ming, Chen, Chien-Wen, Yang, Ya-Sung, Hsieh, Shan-Shan, Tang, Sheng-Hui, Perng, Cherng-Lih, Chang, Feng-Yee, Shang, Hung-Sheng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Impact Journals 2022
Materias:	Research Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217698/ https://www.ncbi.nlm.nih.gov/pubmed/35657641 http://dx.doi.org/10.18632/aging.204095

Ejemplares similares

Emergency SARS-CoV-2 Variants of Concern: Novel Multiplex Real-Time RT-PCR Assay for Rapid Detection and Surveillance
por: Chung, Hsing-Yi, et al.
Publicado: (2022)

Novel automated sample-to-result SARS-CoV-2 laboratory-developed RT-PCR assay for high-throughput testing using LabTurbo AIO 48 system
por: Jian, Ming-Jr, et al.
Publicado: (2021)

Clinical assessment of SARS-CoV-2 antigen rapid detection compared with RT-PCR assay for emerging variants at a high-throughput community testing site in Taiwan
por: Jian, Ming-Jr, et al.
Publicado: (2022)

Multicenter study evaluating one multiplex RT-PCR assay to detect SARS-CoV-2, influenza A/B, and respiratory syncytia virus using the LabTurbo AIO open platform: epidemiological features, automated sample-to-result, and high-throughput testing
por: Chung, Hsing-Yi, et al.
Publicado: (2021)

Novel rapid identification of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by real-time RT-PCR using BD Max Open System in Taiwan
por: Perng, Cherng-Lih, et al.
Publicado: (2020)

Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open system
por: Chung, Hsing-Yi, et al.
Publicado: (2021)

Clinical Comparative Evaluation of the LabTurbo(TM) AIO(®) Reverse Transcription-Polymerase Chain Reaction and World Health Organization-Recommended Assays for the Detection of Emerging SARS-CoV-2 Variants of Concern
por: Chang, Chih-Kai, et al.
Publicado: (2022)

Multicenter study evaluating novel multi-specimen pooling assay for the detection of SARS-CoV-2: High sensitivity and high throughput testing
por: Chung, Hsing-Yi, et al.
Publicado: (2022)

The application of a novel 5-in-1 multiplex reverse transcriptase–polymerase chain reaction assay for rapid detection of SARS-CoV-2 and differentiation between variants of concern
por: Chung, Hsing-Yi, et al.
Publicado: (2023)

Investigation of One Familial Cluster of COVID-19 in Taiwan: Differentiation of Genetic Variation Among Isolates and Implications for Epidemiological Investigation and Surveillance by Genomic Assay
por: Jian, Ming-Jr, et al.
Publicado: (2021)

Genomic analysis of early transmissibility assessment of the D614G mutant strain of SARS-CoV-2 in travelers returning to Taiwan from the United States of America
por: Jian, Ming-Jr, et al.
Publicado: (2021)

Monitoring algorithm of hospitalized patients in a medical center with SARS-CoV-2 (Omicron variant) infection: clinical epidemiological surveillance and immunological assessment
por: Chen, Chi-Sheng, et al.
Publicado: (2023)

Clinical Comparison of Three Sample-to-Answer Systems for Detecting SARS-CoV-2 in B.1.1.7 Lineage Emergence
por: Jian, Ming-Jr, et al.
Publicado: (2021)

SARS-CoV-2 variants with T135I nucleocapsid mutations may affect antigen test performance
por: Jian, Ming-Jr, et al.
Publicado: (2022)

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR
por: Song, Lele, et al.
Publicado: (2016)

Designing In-House SARS-CoV-2 RT-qPCR Assay for Variant of Concerns
por: Ergoren, Mahmut Cerkez, et al.
Publicado: (2022)

Selection of reference genes for RT-qPCR studies in blood of beluga whales (Delphinapterus leucas)
por: Chen, I-Hua, et al.
Publicado: (2016)

Selection of appropriate reference genes for RT-qPCR analysis under abiotic stress and hormone treatment in celery
por: Feng, Kai, et al.
Publicado: (2019)

Linear-hairpin variable primer RT-qPCR for MicroRNA
por: Lan, Lin, et al.
Publicado: (2018)

Critical pediatric neurological illness associated with COVID-19 (Omicron BA.2.3.7 variant) infection in Taiwan: immunological assessment and viral genome analysis in tertiary medical center
por: Chen, Chi-Sheng, et al.
Publicado: (2022)

Effective high-throughput RT-qPCR screening for SARS-CoV-2 infections in children
por: Dewald, Felix, et al.
Publicado: (2022)

Integrating Magnetic-Bead-Based Sample Extraction and Molecular Barcoding for the One-Step Pooled RT-qPCR Assay of Viral Pathogens without Retesting
por: Zhuang, Xinyu, et al.
Publicado: (2023)

Tutorial: Guidelines for Single-Cell RT-qPCR
por: Zucha, Daniel, et al.
Publicado: (2021)

COVID-19 diagnosis by RT-qPCR in alternative specimens
por: Gonçalves, Cássia Cristina Alves, et al.
Publicado: (2021)

Molecular typing and profiling of topoisomerase mutations causing resistance to ciprofloxacin and levofloxacin in Elizabethkingia species
por: Jian, Ming-Jr, et al.
Publicado: (2018)

Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
por: Park, Changwoo, et al.
Publicado: (2023)

High throughput nano-liter RT-qPCR to classify soil contamination using a soil arthropod
por: de Boer, Muriel E, et al.
Publicado: (2011)

High-throughput identification of reference genes for research and clinical RT-qPCR analysis of breast cancer samples
por: Maltseva, Diana V, et al.
Publicado: (2013)

Evaluation of extraction kits and RT-qPCR systems adapted to high-throughput platform for circulating miRNAs
por: Tan, Geok Wee, et al.
Publicado: (2015)

A high-throughput screening RT-qPCR assay for quantifying surrogate markers of immunity from PBMCs
por: Browne, Daniel J., et al.
Publicado: (2022)

Miniature RT–PCR system for diagnosis of RNA-based viruses
por: Liao, Chia-Sheng, et al.
Publicado: (2005)

Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp
por: Mo, Fei, et al.
Publicado: (2014)

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
por: Fout, G. Shay, et al.
Publicado: (2016)

Screening Reliable Reference Genes for RT-qPCR Analysis of Gene Expression in Moringa oleifera
por: Deng, Li-Ting, et al.
Publicado: (2016)

SASqPCR: Robust and Rapid Analysis of RT-qPCR Data in SAS
por: Ling, Daijun
Publicado: (2012)

Improving the standardization of mRNA measurement by RT-qPCR
por: Sanders, Rebecca, et al.
Publicado: (2018)

Rapid and accurate quantification of isomiRs by RT-qPCR
por: Franco, Sandra, et al.
Publicado: (2022)

Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome
por: Nunes, Bruno Tardelli Diniz, et al.
Publicado: (2019)

Statistical concern on evaluating the consistency between gargle samples and nasopharyngeal (NP) swabs in RT-qPCR-based mass screening approach for the diagnosis of COVID-19
por: Li, Ming, et al.
Publicado: (2022)

Seasonal dynamics and co‐occurrence patterns of honey bee pathogens revealed by high‐throughput RT‐qPCR analysis
por: D'Alvise, Paul, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_cugfebf3vrlt9b407knq76at3d