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Far-UV circular dichroism signatures indicate fluorophore labeling induced conformational changes of penetratin

Fluorescent labeling is a broadly utilized approach to assess in vitro and in vivo behavior of biologically active, especially cell-penetrating and antimicrobial peptides. In this communication, far-UV circular dichroism (CD) spectra of penetratin (PEN) fluorophore conjugates reported previously hav...

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Autor principal: Zsila, Ferenc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217886/
https://www.ncbi.nlm.nih.gov/pubmed/35301594
http://dx.doi.org/10.1007/s00726-022-03149-1
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author Zsila, Ferenc
author_facet Zsila, Ferenc
author_sort Zsila, Ferenc
collection PubMed
description Fluorescent labeling is a broadly utilized approach to assess in vitro and in vivo behavior of biologically active, especially cell-penetrating and antimicrobial peptides. In this communication, far-UV circular dichroism (CD) spectra of penetratin (PEN) fluorophore conjugates reported previously have been re-evaluated. Compared to the intrinsically disordered native peptide, rhodamine B and carboxyfluorescein derivatives of free and membrane-bound PEN exhibit extrinsic CD features. Potential sources of these signals displayed above 220 nm are discussed suggesting the contributions of both intra- and intermolecular chiral exciton coupling mechanisms. Careful evaluation of the CD spectra of fluorophore-labeled peptides is a valuable tool for early detection of labeling-provoked structural alterations which in turn may modify the membrane binding and cellular uptake compared to the unconjugated form.
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spelling pubmed-92178862022-06-24 Far-UV circular dichroism signatures indicate fluorophore labeling induced conformational changes of penetratin Zsila, Ferenc Amino Acids Short Communication Fluorescent labeling is a broadly utilized approach to assess in vitro and in vivo behavior of biologically active, especially cell-penetrating and antimicrobial peptides. In this communication, far-UV circular dichroism (CD) spectra of penetratin (PEN) fluorophore conjugates reported previously have been re-evaluated. Compared to the intrinsically disordered native peptide, rhodamine B and carboxyfluorescein derivatives of free and membrane-bound PEN exhibit extrinsic CD features. Potential sources of these signals displayed above 220 nm are discussed suggesting the contributions of both intra- and intermolecular chiral exciton coupling mechanisms. Careful evaluation of the CD spectra of fluorophore-labeled peptides is a valuable tool for early detection of labeling-provoked structural alterations which in turn may modify the membrane binding and cellular uptake compared to the unconjugated form. Springer Vienna 2022-03-18 2022 /pmc/articles/PMC9217886/ /pubmed/35301594 http://dx.doi.org/10.1007/s00726-022-03149-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Short Communication
Zsila, Ferenc
Far-UV circular dichroism signatures indicate fluorophore labeling induced conformational changes of penetratin
title Far-UV circular dichroism signatures indicate fluorophore labeling induced conformational changes of penetratin
title_full Far-UV circular dichroism signatures indicate fluorophore labeling induced conformational changes of penetratin
title_fullStr Far-UV circular dichroism signatures indicate fluorophore labeling induced conformational changes of penetratin
title_full_unstemmed Far-UV circular dichroism signatures indicate fluorophore labeling induced conformational changes of penetratin
title_short Far-UV circular dichroism signatures indicate fluorophore labeling induced conformational changes of penetratin
title_sort far-uv circular dichroism signatures indicate fluorophore labeling induced conformational changes of penetratin
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217886/
https://www.ncbi.nlm.nih.gov/pubmed/35301594
http://dx.doi.org/10.1007/s00726-022-03149-1
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