CF-PPiD technology based on cell-free protein array and proximity biotinylation enzyme for in vitro direct interactome analysis

Protein–protein interaction (PPI) analysis is a key process to understand protein functions. Recently, we constructed a human protein array (20 K human protein beads array) consisting of 19,712 recombinant human proteins produced by a wheat cell-free protein production system. Here, we developed a c...

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Detalles Bibliográficos
Autores principales: Sugiyama, Shusei, Yamada, Kohdai, Denda, Miwako, Yamanaka, Satoshi, Ozawa, Satoshi, Morishita, Ryo, Sawasaki, Tatsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217950/
https://www.ncbi.nlm.nih.gov/pubmed/35732899
http://dx.doi.org/10.1038/s41598-022-14872-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217950/
https://www.ncbi.nlm.nih.gov/pubmed/35732899
http://dx.doi.org/10.1038/s41598-022-14872-w

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