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CF-PPiD technology based on cell-free protein array and proximity biotinylation enzyme for in vitro direct interactome analysis
Protein–protein interaction (PPI) analysis is a key process to understand protein functions. Recently, we constructed a human protein array (20 K human protein beads array) consisting of 19,712 recombinant human proteins produced by a wheat cell-free protein production system. Here, we developed a c...
Autores principales: | Sugiyama, Shusei, Yamada, Kohdai, Denda, Miwako, Yamanaka, Satoshi, Ozawa, Satoshi, Morishita, Ryo, Sawasaki, Tatsuya |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217950/ https://www.ncbi.nlm.nih.gov/pubmed/35732899 http://dx.doi.org/10.1038/s41598-022-14872-w |
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