In vitro analyses of paracrine effects of murine classically activated macrophage on beige adipocyte metabolism

The communication between macrophage and adipocyte plays a critical role in the initiation and development of metabolic inflammation, which is difficult to study in vivo. Here, we provide a step-by-step protocol using differentiated cells to investigate the paracrine effects of classically activated...

Descripción completa

Detalles Bibliográficos
Autores principales: Yao, Jingfei, Wu, Dongmei, Qiu, Yifu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218234/
https://www.ncbi.nlm.nih.gov/pubmed/35755124
http://dx.doi.org/10.1016/j.xpro.2022.101480
_version_ 1784731837666426880
author Yao, Jingfei
Wu, Dongmei
Qiu, Yifu
author_facet Yao, Jingfei
Wu, Dongmei
Qiu, Yifu
author_sort Yao, Jingfei
collection PubMed
description The communication between macrophage and adipocyte plays a critical role in the initiation and development of metabolic inflammation, which is difficult to study in vivo. Here, we provide a step-by-step protocol using differentiated cells to investigate the paracrine effects of classically activated macrophage on beige adipocyte metabolism in vitro. This protocol uses bone-marrow-derived macrophage and SVF-derived UCP1(+) beige adipocyte in a culture model to study immune regulation of adipocyte metabolism by western blot analyses. For complete details on the use and execution of this protocol, please refer to Yao et al. (2021).
format Online
Article
Text
id pubmed-9218234
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-92182342022-06-24 In vitro analyses of paracrine effects of murine classically activated macrophage on beige adipocyte metabolism Yao, Jingfei Wu, Dongmei Qiu, Yifu STAR Protoc Protocol The communication between macrophage and adipocyte plays a critical role in the initiation and development of metabolic inflammation, which is difficult to study in vivo. Here, we provide a step-by-step protocol using differentiated cells to investigate the paracrine effects of classically activated macrophage on beige adipocyte metabolism in vitro. This protocol uses bone-marrow-derived macrophage and SVF-derived UCP1(+) beige adipocyte in a culture model to study immune regulation of adipocyte metabolism by western blot analyses. For complete details on the use and execution of this protocol, please refer to Yao et al. (2021). Elsevier 2022-06-17 /pmc/articles/PMC9218234/ /pubmed/35755124 http://dx.doi.org/10.1016/j.xpro.2022.101480 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Yao, Jingfei
Wu, Dongmei
Qiu, Yifu
In vitro analyses of paracrine effects of murine classically activated macrophage on beige adipocyte metabolism
title In vitro analyses of paracrine effects of murine classically activated macrophage on beige adipocyte metabolism
title_full In vitro analyses of paracrine effects of murine classically activated macrophage on beige adipocyte metabolism
title_fullStr In vitro analyses of paracrine effects of murine classically activated macrophage on beige adipocyte metabolism
title_full_unstemmed In vitro analyses of paracrine effects of murine classically activated macrophage on beige adipocyte metabolism
title_short In vitro analyses of paracrine effects of murine classically activated macrophage on beige adipocyte metabolism
title_sort in vitro analyses of paracrine effects of murine classically activated macrophage on beige adipocyte metabolism
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218234/
https://www.ncbi.nlm.nih.gov/pubmed/35755124
http://dx.doi.org/10.1016/j.xpro.2022.101480
work_keys_str_mv AT yaojingfei invitroanalysesofparacrineeffectsofmurineclassicallyactivatedmacrophageonbeigeadipocytemetabolism
AT wudongmei invitroanalysesofparacrineeffectsofmurineclassicallyactivatedmacrophageonbeigeadipocytemetabolism
AT qiuyifu invitroanalysesofparacrineeffectsofmurineclassicallyactivatedmacrophageonbeigeadipocytemetabolism

Ejemplares similares