Protocol for single-nucleus ATAC sequencing and bioinformatic analysis in frozen human brain tissue

Single-nucleus ATAC sequencing (snATAC-seq) employs a hyperactive Tn5 transposase to gain precise information about the cis-regulatory elements in specific cell types. However, the standard protocol of snATAC-seq is not optimized for all tissues, including the brain. Here, we present a modified prot...

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Detalles Bibliográficos
Autores principales: Shi, Zechuan, Das, Sudeshna, Morabito, Samuel, Miyoshi, Emily, Swarup, Vivek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218237/
https://www.ncbi.nlm.nih.gov/pubmed/35755130
http://dx.doi.org/10.1016/j.xpro.2022.101491

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