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Ultrasensitive detection of exosomal miRNA with PMO-graphene quantum dots-functionalized field-effect transistor biosensor

Compared with the conventional DNA probe immobilization on the planar surface, nanoparticles-based DNA probes enable more RNA molecules to be anchored to the sensor surface, thereby improving the detection sensitivity. In this work, we report phosphorodiamidate morpholino oligomers (PMO)-graphene qu...

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Autores principales: Li, Kun, Tu, Jiyuan, Zhang, Yulin, Jin, Dan, Li, Tingxian, Li, Jiahao, Ni, Wei, Xiao, Meng-Meng, Zhang, Zhi-Yong, Zhang, Guo-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218366/
https://www.ncbi.nlm.nih.gov/pubmed/35754714
http://dx.doi.org/10.1016/j.isci.2022.104522
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author Li, Kun
Tu, Jiyuan
Zhang, Yulin
Jin, Dan
Li, Tingxian
Li, Jiahao
Ni, Wei
Xiao, Meng-Meng
Zhang, Zhi-Yong
Zhang, Guo-Jun
author_facet Li, Kun
Tu, Jiyuan
Zhang, Yulin
Jin, Dan
Li, Tingxian
Li, Jiahao
Ni, Wei
Xiao, Meng-Meng
Zhang, Zhi-Yong
Zhang, Guo-Jun
author_sort Li, Kun
collection PubMed
description Compared with the conventional DNA probe immobilization on the planar surface, nanoparticles-based DNA probes enable more RNA molecules to be anchored to the sensor surface, thereby improving the detection sensitivity. In this work, we report phosphorodiamidate morpholino oligomers (PMO)-graphene quantum dots (GQDs)-functionalized reduced graphene oxide (RGO) field effect transistor (FET) biosensors for ultrasensitive detection of exosomal microRNAs. After the RGO FET sensor was fabricated, polylysine (PLL) film was deposited onto the RGO surface. GQDs-PMO hybrid was prepared and covalently bound to PLL surface, enabling detection of exosomal microRNAs (miRNAs). The method achieved a detection limit as low as 85 aM and high specificity. Furthermore, the FET sensor was able to detect exosomal miRNAs in plasma samples and distinguish breast cancer samples from healthy samples. Compared with other methods, we use GQDs to further improve the sensitivity of FET, making it a potential tool for early diagnosis of breast cancer.
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spelling pubmed-92183662022-06-24 Ultrasensitive detection of exosomal miRNA with PMO-graphene quantum dots-functionalized field-effect transistor biosensor Li, Kun Tu, Jiyuan Zhang, Yulin Jin, Dan Li, Tingxian Li, Jiahao Ni, Wei Xiao, Meng-Meng Zhang, Zhi-Yong Zhang, Guo-Jun iScience Article Compared with the conventional DNA probe immobilization on the planar surface, nanoparticles-based DNA probes enable more RNA molecules to be anchored to the sensor surface, thereby improving the detection sensitivity. In this work, we report phosphorodiamidate morpholino oligomers (PMO)-graphene quantum dots (GQDs)-functionalized reduced graphene oxide (RGO) field effect transistor (FET) biosensors for ultrasensitive detection of exosomal microRNAs. After the RGO FET sensor was fabricated, polylysine (PLL) film was deposited onto the RGO surface. GQDs-PMO hybrid was prepared and covalently bound to PLL surface, enabling detection of exosomal microRNAs (miRNAs). The method achieved a detection limit as low as 85 aM and high specificity. Furthermore, the FET sensor was able to detect exosomal miRNAs in plasma samples and distinguish breast cancer samples from healthy samples. Compared with other methods, we use GQDs to further improve the sensitivity of FET, making it a potential tool for early diagnosis of breast cancer. Elsevier 2022-06-03 /pmc/articles/PMC9218366/ /pubmed/35754714 http://dx.doi.org/10.1016/j.isci.2022.104522 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Li, Kun
Tu, Jiyuan
Zhang, Yulin
Jin, Dan
Li, Tingxian
Li, Jiahao
Ni, Wei
Xiao, Meng-Meng
Zhang, Zhi-Yong
Zhang, Guo-Jun
Ultrasensitive detection of exosomal miRNA with PMO-graphene quantum dots-functionalized field-effect transistor biosensor
title Ultrasensitive detection of exosomal miRNA with PMO-graphene quantum dots-functionalized field-effect transistor biosensor
title_full Ultrasensitive detection of exosomal miRNA with PMO-graphene quantum dots-functionalized field-effect transistor biosensor
title_fullStr Ultrasensitive detection of exosomal miRNA with PMO-graphene quantum dots-functionalized field-effect transistor biosensor
title_full_unstemmed Ultrasensitive detection of exosomal miRNA with PMO-graphene quantum dots-functionalized field-effect transistor biosensor
title_short Ultrasensitive detection of exosomal miRNA with PMO-graphene quantum dots-functionalized field-effect transistor biosensor
title_sort ultrasensitive detection of exosomal mirna with pmo-graphene quantum dots-functionalized field-effect transistor biosensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218366/
https://www.ncbi.nlm.nih.gov/pubmed/35754714
http://dx.doi.org/10.1016/j.isci.2022.104522
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