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Transcriptomics-Based Study of Differentially Expressed Genes Related to Fat Deposition in Tibetan and Yorkshire Pigs

Fat deposition traits are one of the key factors in pig production and breeding. The fat deposition capacity of pigs mainly affects the quality of pork and pig productivity. The aim of this study was to analyze the differential expression of mRNA levels in dorsal adipose tissue of Tibetan and York p...

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Autores principales: Gong, Xinglong, Zheng, Min, Zhang, Jian, Ye, Yourong, Duan, Mengqi, Chamba, Yangzom, Wang, Zhongbin, Shang, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218471/
https://www.ncbi.nlm.nih.gov/pubmed/35754534
http://dx.doi.org/10.3389/fvets.2022.919904
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author Gong, Xinglong
Zheng, Min
Zhang, Jian
Ye, Yourong
Duan, Mengqi
Chamba, Yangzom
Wang, Zhongbin
Shang, Peng
author_facet Gong, Xinglong
Zheng, Min
Zhang, Jian
Ye, Yourong
Duan, Mengqi
Chamba, Yangzom
Wang, Zhongbin
Shang, Peng
author_sort Gong, Xinglong
collection PubMed
description Fat deposition traits are one of the key factors in pig production and breeding. The fat deposition capacity of pigs mainly affects the quality of pork and pig productivity. The aim of this study was to analyze the differential expression of mRNA levels in dorsal adipose tissue of Tibetan and York pigs at different growth stages using transcriptomic data to estimate key genes that regulate fat deposition in pigs. The results showed that a total of 32,747 positively expressed genes were present in the dorsal adipose tissue of the two breeds. Differentially expressed gene (DEG) screening of multiple combinations between the two breeds yielded 324 DEGS. Gene ontology (GO) biofunctional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that these DEGS were mainly involved in lipid metabolic pathways, steroid biosynthetic pathways and lipid biosynthetic processes, sterol biosynthetic processes, brown adipocyte differentiation, and other pathways related to lipid deposition and metabolism. The results showed that ACACA, SLC2A4 and THRSP genes positively regulated the lipid deposition ability and CHPT1 gene negatively regulated the lipid deposition ability in pigs. The results of this experiment suggest a theoretical basis for further studies on the regulatory mechanisms of fat deposition in pigs.
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spelling pubmed-92184712022-06-24 Transcriptomics-Based Study of Differentially Expressed Genes Related to Fat Deposition in Tibetan and Yorkshire Pigs Gong, Xinglong Zheng, Min Zhang, Jian Ye, Yourong Duan, Mengqi Chamba, Yangzom Wang, Zhongbin Shang, Peng Front Vet Sci Veterinary Science Fat deposition traits are one of the key factors in pig production and breeding. The fat deposition capacity of pigs mainly affects the quality of pork and pig productivity. The aim of this study was to analyze the differential expression of mRNA levels in dorsal adipose tissue of Tibetan and York pigs at different growth stages using transcriptomic data to estimate key genes that regulate fat deposition in pigs. The results showed that a total of 32,747 positively expressed genes were present in the dorsal adipose tissue of the two breeds. Differentially expressed gene (DEG) screening of multiple combinations between the two breeds yielded 324 DEGS. Gene ontology (GO) biofunctional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that these DEGS were mainly involved in lipid metabolic pathways, steroid biosynthetic pathways and lipid biosynthetic processes, sterol biosynthetic processes, brown adipocyte differentiation, and other pathways related to lipid deposition and metabolism. The results showed that ACACA, SLC2A4 and THRSP genes positively regulated the lipid deposition ability and CHPT1 gene negatively regulated the lipid deposition ability in pigs. The results of this experiment suggest a theoretical basis for further studies on the regulatory mechanisms of fat deposition in pigs. Frontiers Media S.A. 2022-06-09 /pmc/articles/PMC9218471/ /pubmed/35754534 http://dx.doi.org/10.3389/fvets.2022.919904 Text en Copyright © 2022 Gong, Zheng, Zhang, Ye, Duan, Chamba, Wang and Shang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Gong, Xinglong
Zheng, Min
Zhang, Jian
Ye, Yourong
Duan, Mengqi
Chamba, Yangzom
Wang, Zhongbin
Shang, Peng
Transcriptomics-Based Study of Differentially Expressed Genes Related to Fat Deposition in Tibetan and Yorkshire Pigs
title Transcriptomics-Based Study of Differentially Expressed Genes Related to Fat Deposition in Tibetan and Yorkshire Pigs
title_full Transcriptomics-Based Study of Differentially Expressed Genes Related to Fat Deposition in Tibetan and Yorkshire Pigs
title_fullStr Transcriptomics-Based Study of Differentially Expressed Genes Related to Fat Deposition in Tibetan and Yorkshire Pigs
title_full_unstemmed Transcriptomics-Based Study of Differentially Expressed Genes Related to Fat Deposition in Tibetan and Yorkshire Pigs
title_short Transcriptomics-Based Study of Differentially Expressed Genes Related to Fat Deposition in Tibetan and Yorkshire Pigs
title_sort transcriptomics-based study of differentially expressed genes related to fat deposition in tibetan and yorkshire pigs
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218471/
https://www.ncbi.nlm.nih.gov/pubmed/35754534
http://dx.doi.org/10.3389/fvets.2022.919904
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