Cargando…

Expression and Clinical Significance of the m6A RNA-Binding Proteins YTHDF2 in Peripheral Blood Mononuclear Cells From New-Onset Ankylosing Spondylitis

This study has focused on determining the association of m6A methyltransferase [methyltransferase-like 3 (METTL3), methyltransferase-like 14 (METTL14), and Wilms tumor 1-associating protein (WTAP)], demethylase [fat mass and obesity-associated protein (FTO) and alkylation repair homolog protein 5 (A...

Descripción completa

Detalles Bibliográficos
Autores principales: Luo, Qing, Guo, Yongqin, Xiao, Qiuyun, Fu, Biqi, Zhang, Lu, Guo, Yang, Huang, Zikun, Li, Junming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218531/
https://www.ncbi.nlm.nih.gov/pubmed/35755020
http://dx.doi.org/10.3389/fmed.2022.922219
_version_ 1784731912052408320
author Luo, Qing
Guo, Yongqin
Xiao, Qiuyun
Fu, Biqi
Zhang, Lu
Guo, Yang
Huang, Zikun
Li, Junming
author_facet Luo, Qing
Guo, Yongqin
Xiao, Qiuyun
Fu, Biqi
Zhang, Lu
Guo, Yang
Huang, Zikun
Li, Junming
author_sort Luo, Qing
collection PubMed
description This study has focused on determining the association of m6A methyltransferase [methyltransferase-like 3 (METTL3), methyltransferase-like 14 (METTL14), and Wilms tumor 1-associating protein (WTAP)], demethylase [fat mass and obesity-associated protein (FTO) and alkylation repair homolog protein 5 (ALKBH5)], RNA-binding proteins [YT521-B homology domains 2 (YTHDF2)], and ankylosing spondylitis (AS). A total of 154 specimens, containing 79 patients with new-onset AS and 75 healthy controls (HCs), participated in the study. The mRNA expressions of these m6A methyltransferase, demethylase, and RNA-binding protein in peripheral blood mononuclear cells (PBMCs) were detected by quantitative real-time PCR (qRT-PCR). The data showed that the mRNA expressions of YTHDF2 and ALKBH5 in PBMC from patients with new-onset AS were significantly decreased, and there was a positive correlation between RNA-binding proteins (YTHDF2) and demethylase (ALKBH5) in patients with new-onset AS. Logistic regression analysis demonstrated that the expression of YTHDF2 mRNA in PBMC is a risk factor of AS. Receiver operating characteristic (ROC) analysis of the area under the curve (AUC) for mRNA YTHDF2 in new-onset AS and HC was 0.692, with a cutoff value of <0.8724, a sensitivity of 67%, and a specificity of 63%. Moreover, we constructed a novel predictive model based on a combination of mRNA YTHDF2 and systemic immune-inflammation index (SII) for AS diagnosis (AUC = 0.865, sensitivity = 79.45%, specificity = 84.00%), and the predictive model correlated with the activity and severity of AS. This study indicates that the mRNA expression of YTHDF2 in PBMC may be involved in AS pathogenesis and a predictive model based on a combination of mRNA YTHDF2 and SII acts as a marker for diagnosis and progression of diseases.
format Online
Article
Text
id pubmed-9218531
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92185312022-06-24 Expression and Clinical Significance of the m6A RNA-Binding Proteins YTHDF2 in Peripheral Blood Mononuclear Cells From New-Onset Ankylosing Spondylitis Luo, Qing Guo, Yongqin Xiao, Qiuyun Fu, Biqi Zhang, Lu Guo, Yang Huang, Zikun Li, Junming Front Med (Lausanne) Medicine This study has focused on determining the association of m6A methyltransferase [methyltransferase-like 3 (METTL3), methyltransferase-like 14 (METTL14), and Wilms tumor 1-associating protein (WTAP)], demethylase [fat mass and obesity-associated protein (FTO) and alkylation repair homolog protein 5 (ALKBH5)], RNA-binding proteins [YT521-B homology domains 2 (YTHDF2)], and ankylosing spondylitis (AS). A total of 154 specimens, containing 79 patients with new-onset AS and 75 healthy controls (HCs), participated in the study. The mRNA expressions of these m6A methyltransferase, demethylase, and RNA-binding protein in peripheral blood mononuclear cells (PBMCs) were detected by quantitative real-time PCR (qRT-PCR). The data showed that the mRNA expressions of YTHDF2 and ALKBH5 in PBMC from patients with new-onset AS were significantly decreased, and there was a positive correlation between RNA-binding proteins (YTHDF2) and demethylase (ALKBH5) in patients with new-onset AS. Logistic regression analysis demonstrated that the expression of YTHDF2 mRNA in PBMC is a risk factor of AS. Receiver operating characteristic (ROC) analysis of the area under the curve (AUC) for mRNA YTHDF2 in new-onset AS and HC was 0.692, with a cutoff value of <0.8724, a sensitivity of 67%, and a specificity of 63%. Moreover, we constructed a novel predictive model based on a combination of mRNA YTHDF2 and systemic immune-inflammation index (SII) for AS diagnosis (AUC = 0.865, sensitivity = 79.45%, specificity = 84.00%), and the predictive model correlated with the activity and severity of AS. This study indicates that the mRNA expression of YTHDF2 in PBMC may be involved in AS pathogenesis and a predictive model based on a combination of mRNA YTHDF2 and SII acts as a marker for diagnosis and progression of diseases. Frontiers Media S.A. 2022-06-09 /pmc/articles/PMC9218531/ /pubmed/35755020 http://dx.doi.org/10.3389/fmed.2022.922219 Text en Copyright © 2022 Luo, Guo, Xiao, Fu, Zhang, Guo, Huang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Luo, Qing
Guo, Yongqin
Xiao, Qiuyun
Fu, Biqi
Zhang, Lu
Guo, Yang
Huang, Zikun
Li, Junming
Expression and Clinical Significance of the m6A RNA-Binding Proteins YTHDF2 in Peripheral Blood Mononuclear Cells From New-Onset Ankylosing Spondylitis
title Expression and Clinical Significance of the m6A RNA-Binding Proteins YTHDF2 in Peripheral Blood Mononuclear Cells From New-Onset Ankylosing Spondylitis
title_full Expression and Clinical Significance of the m6A RNA-Binding Proteins YTHDF2 in Peripheral Blood Mononuclear Cells From New-Onset Ankylosing Spondylitis
title_fullStr Expression and Clinical Significance of the m6A RNA-Binding Proteins YTHDF2 in Peripheral Blood Mononuclear Cells From New-Onset Ankylosing Spondylitis
title_full_unstemmed Expression and Clinical Significance of the m6A RNA-Binding Proteins YTHDF2 in Peripheral Blood Mononuclear Cells From New-Onset Ankylosing Spondylitis
title_short Expression and Clinical Significance of the m6A RNA-Binding Proteins YTHDF2 in Peripheral Blood Mononuclear Cells From New-Onset Ankylosing Spondylitis
title_sort expression and clinical significance of the m6a rna-binding proteins ythdf2 in peripheral blood mononuclear cells from new-onset ankylosing spondylitis
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218531/
https://www.ncbi.nlm.nih.gov/pubmed/35755020
http://dx.doi.org/10.3389/fmed.2022.922219
work_keys_str_mv AT luoqing expressionandclinicalsignificanceofthem6arnabindingproteinsythdf2inperipheralbloodmononuclearcellsfromnewonsetankylosingspondylitis
AT guoyongqin expressionandclinicalsignificanceofthem6arnabindingproteinsythdf2inperipheralbloodmononuclearcellsfromnewonsetankylosingspondylitis
AT xiaoqiuyun expressionandclinicalsignificanceofthem6arnabindingproteinsythdf2inperipheralbloodmononuclearcellsfromnewonsetankylosingspondylitis
AT fubiqi expressionandclinicalsignificanceofthem6arnabindingproteinsythdf2inperipheralbloodmononuclearcellsfromnewonsetankylosingspondylitis
AT zhanglu expressionandclinicalsignificanceofthem6arnabindingproteinsythdf2inperipheralbloodmononuclearcellsfromnewonsetankylosingspondylitis
AT guoyang expressionandclinicalsignificanceofthem6arnabindingproteinsythdf2inperipheralbloodmononuclearcellsfromnewonsetankylosingspondylitis
AT huangzikun expressionandclinicalsignificanceofthem6arnabindingproteinsythdf2inperipheralbloodmononuclearcellsfromnewonsetankylosingspondylitis
AT lijunming expressionandclinicalsignificanceofthem6arnabindingproteinsythdf2inperipheralbloodmononuclearcellsfromnewonsetankylosingspondylitis