Cargando…
Identification and Characterization of Shaker K(+) Channel Gene Family in Foxtail Millet (Setaria italica) and Their Role in Stress Response
Potassium (K(+)) is one of the indispensable elements in plant growth and development. The Shaker K(+) channel protein family is involved in plant K(+) uptake and distribution. Foxtail millet (Setaria italica), as an important crop, has strong tolerance and adaptability to abiotic stresses. However,...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218596/ https://www.ncbi.nlm.nih.gov/pubmed/35755660 http://dx.doi.org/10.3389/fpls.2022.907635 |
_version_ | 1784731927208525824 |
---|---|
author | Zhang, Ben Guo, Yue Wang, Hui Wang, Xiaoxia Lv, Mengtao Yang, Pu Zhang, Lizhen |
author_facet | Zhang, Ben Guo, Yue Wang, Hui Wang, Xiaoxia Lv, Mengtao Yang, Pu Zhang, Lizhen |
author_sort | Zhang, Ben |
collection | PubMed |
description | Potassium (K(+)) is one of the indispensable elements in plant growth and development. The Shaker K(+) channel protein family is involved in plant K(+) uptake and distribution. Foxtail millet (Setaria italica), as an important crop, has strong tolerance and adaptability to abiotic stresses. However, no systematic study focused on the Shaker K(+) channel family in foxtail millet. Here, ten Shaker K(+) channel genes in foxtail millet were identified and divided into five groups through phylogenetic analysis. Gene structures, chromosome locations, cis-acting regulatory elements in promoter, and post-translation modification sites of Shaker K(+) channels were analyzed. In silico analysis of transcript level demonstrated that the expression of Shaker K(+) channel genes was tissue or developmental stage specific. The transcription levels of Shaker K(+) channel genes in foxtail millet under different abiotic stresses (cold, heat, NaCl, and PEG) and phytohormones (6-BA, BR, MJ, IAA, NAA, GA3, SA, and ABA) treatments at 0, 12, and 24 h were detected by qRT-PCR. The results showed that SiAKT1, SiKAT3, SiGORK, and SiSKOR were worth further research due to their significant responses after most treatments. The yeast complementation assay verified the inward K(+) transport activities of detectable Shaker K(+) channels. Finally, we found interactions between SiKAT2 and SiSNARE proteins. Compared to research in Arabidopsis, our results showed a difference in SYP121 related Shaker K(+) channel regulation mechanism in foxtail millet. Our results indicate that Shaker K(+) channels play important roles in foxtail millet and provide theoretical support for further exploring the K(+) absorption mechanism of foxtail millet under abiotic stress. |
format | Online Article Text |
id | pubmed-9218596 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92185962022-06-24 Identification and Characterization of Shaker K(+) Channel Gene Family in Foxtail Millet (Setaria italica) and Their Role in Stress Response Zhang, Ben Guo, Yue Wang, Hui Wang, Xiaoxia Lv, Mengtao Yang, Pu Zhang, Lizhen Front Plant Sci Plant Science Potassium (K(+)) is one of the indispensable elements in plant growth and development. The Shaker K(+) channel protein family is involved in plant K(+) uptake and distribution. Foxtail millet (Setaria italica), as an important crop, has strong tolerance and adaptability to abiotic stresses. However, no systematic study focused on the Shaker K(+) channel family in foxtail millet. Here, ten Shaker K(+) channel genes in foxtail millet were identified and divided into five groups through phylogenetic analysis. Gene structures, chromosome locations, cis-acting regulatory elements in promoter, and post-translation modification sites of Shaker K(+) channels were analyzed. In silico analysis of transcript level demonstrated that the expression of Shaker K(+) channel genes was tissue or developmental stage specific. The transcription levels of Shaker K(+) channel genes in foxtail millet under different abiotic stresses (cold, heat, NaCl, and PEG) and phytohormones (6-BA, BR, MJ, IAA, NAA, GA3, SA, and ABA) treatments at 0, 12, and 24 h were detected by qRT-PCR. The results showed that SiAKT1, SiKAT3, SiGORK, and SiSKOR were worth further research due to their significant responses after most treatments. The yeast complementation assay verified the inward K(+) transport activities of detectable Shaker K(+) channels. Finally, we found interactions between SiKAT2 and SiSNARE proteins. Compared to research in Arabidopsis, our results showed a difference in SYP121 related Shaker K(+) channel regulation mechanism in foxtail millet. Our results indicate that Shaker K(+) channels play important roles in foxtail millet and provide theoretical support for further exploring the K(+) absorption mechanism of foxtail millet under abiotic stress. Frontiers Media S.A. 2022-06-09 /pmc/articles/PMC9218596/ /pubmed/35755660 http://dx.doi.org/10.3389/fpls.2022.907635 Text en Copyright © 2022 Zhang, Guo, Wang, Wang, Lv, Yang and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Zhang, Ben Guo, Yue Wang, Hui Wang, Xiaoxia Lv, Mengtao Yang, Pu Zhang, Lizhen Identification and Characterization of Shaker K(+) Channel Gene Family in Foxtail Millet (Setaria italica) and Their Role in Stress Response |
title | Identification and Characterization of Shaker K(+) Channel Gene Family in Foxtail Millet (Setaria italica) and Their Role in Stress Response |
title_full | Identification and Characterization of Shaker K(+) Channel Gene Family in Foxtail Millet (Setaria italica) and Their Role in Stress Response |
title_fullStr | Identification and Characterization of Shaker K(+) Channel Gene Family in Foxtail Millet (Setaria italica) and Their Role in Stress Response |
title_full_unstemmed | Identification and Characterization of Shaker K(+) Channel Gene Family in Foxtail Millet (Setaria italica) and Their Role in Stress Response |
title_short | Identification and Characterization of Shaker K(+) Channel Gene Family in Foxtail Millet (Setaria italica) and Their Role in Stress Response |
title_sort | identification and characterization of shaker k(+) channel gene family in foxtail millet (setaria italica) and their role in stress response |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218596/ https://www.ncbi.nlm.nih.gov/pubmed/35755660 http://dx.doi.org/10.3389/fpls.2022.907635 |
work_keys_str_mv | AT zhangben identificationandcharacterizationofshakerkchannelgenefamilyinfoxtailmilletsetariaitalicaandtheirroleinstressresponse AT guoyue identificationandcharacterizationofshakerkchannelgenefamilyinfoxtailmilletsetariaitalicaandtheirroleinstressresponse AT wanghui identificationandcharacterizationofshakerkchannelgenefamilyinfoxtailmilletsetariaitalicaandtheirroleinstressresponse AT wangxiaoxia identificationandcharacterizationofshakerkchannelgenefamilyinfoxtailmilletsetariaitalicaandtheirroleinstressresponse AT lvmengtao identificationandcharacterizationofshakerkchannelgenefamilyinfoxtailmilletsetariaitalicaandtheirroleinstressresponse AT yangpu identificationandcharacterizationofshakerkchannelgenefamilyinfoxtailmilletsetariaitalicaandtheirroleinstressresponse AT zhanglizhen identificationandcharacterizationofshakerkchannelgenefamilyinfoxtailmilletsetariaitalicaandtheirroleinstressresponse |