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Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns

Physical interactions between cells and micro/nanometer-sized architecture presented in an extracellular matrix (ECM) environment significantly influence cell adhesion and morphology, often facilitating the incidence of diseases, such as cancer invasion and metastasis. Sensing and responding to the...

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Autores principales: Kim, Kyung Ah, Vellampatti, Srivithya, Kim, Byoung Choul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218603/
https://www.ncbi.nlm.nih.gov/pubmed/35755806
http://dx.doi.org/10.3389/fmolb.2022.825970
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author Kim, Kyung Ah
Vellampatti, Srivithya
Kim, Byoung Choul
author_facet Kim, Kyung Ah
Vellampatti, Srivithya
Kim, Byoung Choul
author_sort Kim, Kyung Ah
collection PubMed
description Physical interactions between cells and micro/nanometer-sized architecture presented in an extracellular matrix (ECM) environment significantly influence cell adhesion and morphology, often facilitating the incidence of diseases, such as cancer invasion and metastasis. Sensing and responding to the topographical cues are deeply associated with a physical interplay between integrins, ligands, and mechanical force transmission, ultimately determining diverse cell behavior. Thus, how the tension applied to the integrin-ligand bonds controls cells’ response to the topographical cues needs to be elucidated through quantitative analysis. Here, in this brief research report, we reported a novel platform, termed “topo-tension gauge tether (TGT),” to visualize single-molecule force applied to the integrin-ligand on the aligned anisotropic nanopatterns. Using the topo-TGT assay, first, topography-induced adhesion and morphology of cancerous and normal cells were compared with the pre-defined peak integrin tension. Next, spatial integrin tensions underneath cells were identified using reconstructed integrin tension maps. As a result, we characterized each cell’s capability to comply with nanotopographies and the magnitude of the spatial integrin tension. Altogether, the quantitative information on integrin tension will be a valuable basis for understanding the biophysical mechanisms underlying the force balance influencing adhesion to the topographical cues.
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spelling pubmed-92186032022-06-24 Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns Kim, Kyung Ah Vellampatti, Srivithya Kim, Byoung Choul Front Mol Biosci Molecular Biosciences Physical interactions between cells and micro/nanometer-sized architecture presented in an extracellular matrix (ECM) environment significantly influence cell adhesion and morphology, often facilitating the incidence of diseases, such as cancer invasion and metastasis. Sensing and responding to the topographical cues are deeply associated with a physical interplay between integrins, ligands, and mechanical force transmission, ultimately determining diverse cell behavior. Thus, how the tension applied to the integrin-ligand bonds controls cells’ response to the topographical cues needs to be elucidated through quantitative analysis. Here, in this brief research report, we reported a novel platform, termed “topo-tension gauge tether (TGT),” to visualize single-molecule force applied to the integrin-ligand on the aligned anisotropic nanopatterns. Using the topo-TGT assay, first, topography-induced adhesion and morphology of cancerous and normal cells were compared with the pre-defined peak integrin tension. Next, spatial integrin tensions underneath cells were identified using reconstructed integrin tension maps. As a result, we characterized each cell’s capability to comply with nanotopographies and the magnitude of the spatial integrin tension. Altogether, the quantitative information on integrin tension will be a valuable basis for understanding the biophysical mechanisms underlying the force balance influencing adhesion to the topographical cues. Frontiers Media S.A. 2022-06-09 /pmc/articles/PMC9218603/ /pubmed/35755806 http://dx.doi.org/10.3389/fmolb.2022.825970 Text en Copyright © 2022 Kim, Vellampatti and Kim. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Kim, Kyung Ah
Vellampatti, Srivithya
Kim, Byoung Choul
Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns
title Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns
title_full Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns
title_fullStr Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns
title_full_unstemmed Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns
title_short Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns
title_sort characterization of integrin molecular tension of human breast cancer cells on anisotropic nanopatterns
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218603/
https://www.ncbi.nlm.nih.gov/pubmed/35755806
http://dx.doi.org/10.3389/fmolb.2022.825970
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