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Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors

An effective wound management strategy needs accurate assessment of wound status throughout the whole healing process. This can be achieved by examining molecular biomarkers including proteins, DNAs, and RNAs. However, existing methods for quantifying these biomarkers such as immunohistochemistry an...

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Autores principales: Hwang, Jangsun, Seo, Youngmin, Jeong, Daun, Ning, Xiaoyu, Wiraja, Christian, Yang, Lixia, Tan, Chew Teng, Lee, Jinhyuck, Kim, Yesol, Kim, Ji Won, Kim, Dai Hyun, Choi, Jonghoon, Lim, Chin Yan, Pu, Kanyi, Jang, Woo Young, Xu, Chenjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218655/
https://www.ncbi.nlm.nih.gov/pubmed/35460189
http://dx.doi.org/10.1002/advs.202104835
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author Hwang, Jangsun
Seo, Youngmin
Jeong, Daun
Ning, Xiaoyu
Wiraja, Christian
Yang, Lixia
Tan, Chew Teng
Lee, Jinhyuck
Kim, Yesol
Kim, Ji Won
Kim, Dai Hyun
Choi, Jonghoon
Lim, Chin Yan
Pu, Kanyi
Jang, Woo Young
Xu, Chenjie
author_facet Hwang, Jangsun
Seo, Youngmin
Jeong, Daun
Ning, Xiaoyu
Wiraja, Christian
Yang, Lixia
Tan, Chew Teng
Lee, Jinhyuck
Kim, Yesol
Kim, Ji Won
Kim, Dai Hyun
Choi, Jonghoon
Lim, Chin Yan
Pu, Kanyi
Jang, Woo Young
Xu, Chenjie
author_sort Hwang, Jangsun
collection PubMed
description An effective wound management strategy needs accurate assessment of wound status throughout the whole healing process. This can be achieved by examining molecular biomarkers including proteins, DNAs, and RNAs. However, existing methods for quantifying these biomarkers such as immunohistochemistry and quantitative polymerase chain reaction are usually laborious, resource‐intensive, and disruptive. This article reports the development and utilization of mRNA nanosensors (i.e., NanoFlare) that are topically applied on cutaneous wounds to reveal the healing status through targeted and semi‐quantitative examination of the mRNA biomarkers in skin cells. In 2D and 3D in vitro models, the efficacy and efficiency of these nanosensors are demonstrated in revealing the dynamic changes of mRNA biomarkers for different stages of wound development. In mouse models, this platform permits the tracking and identification of wound healing stages and a normal and diabetic wound healing process by wound healing index in real time.
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spelling pubmed-92186552022-06-29 Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors Hwang, Jangsun Seo, Youngmin Jeong, Daun Ning, Xiaoyu Wiraja, Christian Yang, Lixia Tan, Chew Teng Lee, Jinhyuck Kim, Yesol Kim, Ji Won Kim, Dai Hyun Choi, Jonghoon Lim, Chin Yan Pu, Kanyi Jang, Woo Young Xu, Chenjie Adv Sci (Weinh) Research Articles An effective wound management strategy needs accurate assessment of wound status throughout the whole healing process. This can be achieved by examining molecular biomarkers including proteins, DNAs, and RNAs. However, existing methods for quantifying these biomarkers such as immunohistochemistry and quantitative polymerase chain reaction are usually laborious, resource‐intensive, and disruptive. This article reports the development and utilization of mRNA nanosensors (i.e., NanoFlare) that are topically applied on cutaneous wounds to reveal the healing status through targeted and semi‐quantitative examination of the mRNA biomarkers in skin cells. In 2D and 3D in vitro models, the efficacy and efficiency of these nanosensors are demonstrated in revealing the dynamic changes of mRNA biomarkers for different stages of wound development. In mouse models, this platform permits the tracking and identification of wound healing stages and a normal and diabetic wound healing process by wound healing index in real time. John Wiley and Sons Inc. 2022-04-22 /pmc/articles/PMC9218655/ /pubmed/35460189 http://dx.doi.org/10.1002/advs.202104835 Text en © 2022 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Hwang, Jangsun
Seo, Youngmin
Jeong, Daun
Ning, Xiaoyu
Wiraja, Christian
Yang, Lixia
Tan, Chew Teng
Lee, Jinhyuck
Kim, Yesol
Kim, Ji Won
Kim, Dai Hyun
Choi, Jonghoon
Lim, Chin Yan
Pu, Kanyi
Jang, Woo Young
Xu, Chenjie
Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors
title Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors
title_full Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors
title_fullStr Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors
title_full_unstemmed Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors
title_short Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors
title_sort monitoring wound healing with topically applied optical nanoflare mrna nanosensors
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218655/
https://www.ncbi.nlm.nih.gov/pubmed/35460189
http://dx.doi.org/10.1002/advs.202104835
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