Cargando…

The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona

Butenyl-spinosyn, a highly effective biological insecticide, is produced by Saccharopolyspora pogona. However, its application has been severely hampered by its low yield. Recent studies have shown that PhoU plays a pivotal role in regulating cell growth, secondary metabolite biosynthesis and intrac...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Jianli, Chen, Jianming, Liu, Yang, Hu, Jinjuan, Xia, Ziyuan, Li, Xiaomin, He, Haocheng, Rang, Jie, Sun, Yunjun, Yu, Ziquan, Cui, Jun, Xia, Liqiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218956/
https://www.ncbi.nlm.nih.gov/pubmed/35756073
http://dx.doi.org/10.3389/fmicb.2022.904627
_version_ 1784732006339313664
author Tang, Jianli
Chen, Jianming
Liu, Yang
Hu, Jinjuan
Xia, Ziyuan
Li, Xiaomin
He, Haocheng
Rang, Jie
Sun, Yunjun
Yu, Ziquan
Cui, Jun
Xia, Liqiu
author_facet Tang, Jianli
Chen, Jianming
Liu, Yang
Hu, Jinjuan
Xia, Ziyuan
Li, Xiaomin
He, Haocheng
Rang, Jie
Sun, Yunjun
Yu, Ziquan
Cui, Jun
Xia, Liqiu
author_sort Tang, Jianli
collection PubMed
description Butenyl-spinosyn, a highly effective biological insecticide, is produced by Saccharopolyspora pogona. However, its application has been severely hampered by its low yield. Recent studies have shown that PhoU plays a pivotal role in regulating cell growth, secondary metabolite biosynthesis and intracellular phosphate levels. Nevertheless, the function of PhoU remains ambiguous in S. pogona. In this study, we investigated the effects of PhoU on the growth and the butenyl-spinosyn biosynthesis of S. pogona by constructing the mutants. Overexpression of phoU increased the production of butenyl-spinosyn to 2.2-fold that of the wild-type strain. However, the phoU deletion resulted in a severe imbalance of intracellular phosphate levels, and suppression of the growth and butenyl-spinosyn biosynthesis. Quantitative Real-time PCR (qRT-PCR) analysis, distinctive protein detection and mass spectrometry revealed that PhoU widely regulated primary metabolism, energy metabolism and DNA repair, which implied that PhoU influences the growth and butenyl-spinosyn biosynthesis of S. pogona as a global regulator.
format Online
Article
Text
id pubmed-9218956
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92189562022-06-24 The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona Tang, Jianli Chen, Jianming Liu, Yang Hu, Jinjuan Xia, Ziyuan Li, Xiaomin He, Haocheng Rang, Jie Sun, Yunjun Yu, Ziquan Cui, Jun Xia, Liqiu Front Microbiol Microbiology Butenyl-spinosyn, a highly effective biological insecticide, is produced by Saccharopolyspora pogona. However, its application has been severely hampered by its low yield. Recent studies have shown that PhoU plays a pivotal role in regulating cell growth, secondary metabolite biosynthesis and intracellular phosphate levels. Nevertheless, the function of PhoU remains ambiguous in S. pogona. In this study, we investigated the effects of PhoU on the growth and the butenyl-spinosyn biosynthesis of S. pogona by constructing the mutants. Overexpression of phoU increased the production of butenyl-spinosyn to 2.2-fold that of the wild-type strain. However, the phoU deletion resulted in a severe imbalance of intracellular phosphate levels, and suppression of the growth and butenyl-spinosyn biosynthesis. Quantitative Real-time PCR (qRT-PCR) analysis, distinctive protein detection and mass spectrometry revealed that PhoU widely regulated primary metabolism, energy metabolism and DNA repair, which implied that PhoU influences the growth and butenyl-spinosyn biosynthesis of S. pogona as a global regulator. Frontiers Media S.A. 2022-06-09 /pmc/articles/PMC9218956/ /pubmed/35756073 http://dx.doi.org/10.3389/fmicb.2022.904627 Text en Copyright © 2022 Tang, Chen, Liu, Hu, Xia, Li, He, Rang, Sun, Yu, Cui and Xia. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Tang, Jianli
Chen, Jianming
Liu, Yang
Hu, Jinjuan
Xia, Ziyuan
Li, Xiaomin
He, Haocheng
Rang, Jie
Sun, Yunjun
Yu, Ziquan
Cui, Jun
Xia, Liqiu
The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona
title The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona
title_full The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona
title_fullStr The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona
title_full_unstemmed The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona
title_short The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona
title_sort global regulator phou positively controls growth and butenyl-spinosyn biosynthesis in saccharopolyspora pogona
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218956/
https://www.ncbi.nlm.nih.gov/pubmed/35756073
http://dx.doi.org/10.3389/fmicb.2022.904627
work_keys_str_mv AT tangjianli theglobalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT chenjianming theglobalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT liuyang theglobalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT hujinjuan theglobalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT xiaziyuan theglobalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT lixiaomin theglobalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT hehaocheng theglobalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT rangjie theglobalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT sunyunjun theglobalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT yuziquan theglobalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT cuijun theglobalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT xialiqiu theglobalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT tangjianli globalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT chenjianming globalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT liuyang globalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT hujinjuan globalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT xiaziyuan globalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT lixiaomin globalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT hehaocheng globalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT rangjie globalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT sunyunjun globalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT yuziquan globalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT cuijun globalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona
AT xialiqiu globalregulatorphoupositivelycontrolsgrowthandbutenylspinosynbiosynthesisinsaccharopolysporapogona