Quantitative glycoproteomics of high-density lipoproteins

In this work, we developed a targeted glycoproteomic method to monitor the site-specific glycoprofiles and quantities of the most abundant HDL-associated proteins using Orbitrap LC-MS for (glyco)peptide target discovery and QqQ LC-MS for quantitative analysis. We conducted a pilot study using the wo...

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Detalles Bibliográficos
Autores principales: Tang, Xinyu, Wong, Maurice, Tena, Jennyfer, Zhu, Chenghao, Rhodes, Christopher, Zhou, Qingwen, Vinjamuri, Anita, Oloumi, Armin, Boddu, Sucharita, Luxardi, Guillaume, Maverakis, Emanual, Lebrilla, Carlito B., Zivkovic, Angela M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218963/
https://www.ncbi.nlm.nih.gov/pubmed/35799915
http://dx.doi.org/10.1039/d2ra02294j
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author Tang, Xinyu
Wong, Maurice
Tena, Jennyfer
Zhu, Chenghao
Rhodes, Christopher
Zhou, Qingwen
Vinjamuri, Anita
Oloumi, Armin
Boddu, Sucharita
Luxardi, Guillaume
Maverakis, Emanual
Lebrilla, Carlito B.
Zivkovic, Angela M.
author_facet Tang, Xinyu
Wong, Maurice
Tena, Jennyfer
Zhu, Chenghao
Rhodes, Christopher
Zhou, Qingwen
Vinjamuri, Anita
Oloumi, Armin
Boddu, Sucharita
Luxardi, Guillaume
Maverakis, Emanual
Lebrilla, Carlito B.
Zivkovic, Angela M.
author_sort Tang, Xinyu
collection PubMed
description In this work, we developed a targeted glycoproteomic method to monitor the site-specific glycoprofiles and quantities of the most abundant HDL-associated proteins using Orbitrap LC-MS for (glyco)peptide target discovery and QqQ LC-MS for quantitative analysis. We conducted a pilot study using the workflow to determine whether HDL protein glycoprofiles are altered in healthy human participants in response to dietary glycan supplementation.
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spelling pubmed-92189632022-07-06 Quantitative glycoproteomics of high-density lipoproteins Tang, Xinyu Wong, Maurice Tena, Jennyfer Zhu, Chenghao Rhodes, Christopher Zhou, Qingwen Vinjamuri, Anita Oloumi, Armin Boddu, Sucharita Luxardi, Guillaume Maverakis, Emanual Lebrilla, Carlito B. Zivkovic, Angela M. RSC Adv Chemistry In this work, we developed a targeted glycoproteomic method to monitor the site-specific glycoprofiles and quantities of the most abundant HDL-associated proteins using Orbitrap LC-MS for (glyco)peptide target discovery and QqQ LC-MS for quantitative analysis. We conducted a pilot study using the workflow to determine whether HDL protein glycoprofiles are altered in healthy human participants in response to dietary glycan supplementation. The Royal Society of Chemistry 2022-06-23 /pmc/articles/PMC9218963/ /pubmed/35799915 http://dx.doi.org/10.1039/d2ra02294j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Tang, Xinyu
Wong, Maurice
Tena, Jennyfer
Zhu, Chenghao
Rhodes, Christopher
Zhou, Qingwen
Vinjamuri, Anita
Oloumi, Armin
Boddu, Sucharita
Luxardi, Guillaume
Maverakis, Emanual
Lebrilla, Carlito B.
Zivkovic, Angela M.
Quantitative glycoproteomics of high-density lipoproteins
title Quantitative glycoproteomics of high-density lipoproteins
title_full Quantitative glycoproteomics of high-density lipoproteins
title_fullStr Quantitative glycoproteomics of high-density lipoproteins
title_full_unstemmed Quantitative glycoproteomics of high-density lipoproteins
title_short Quantitative glycoproteomics of high-density lipoproteins
title_sort quantitative glycoproteomics of high-density lipoproteins
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218963/
https://www.ncbi.nlm.nih.gov/pubmed/35799915
http://dx.doi.org/10.1039/d2ra02294j
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