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Molecular Epidemiology and Genetic Diversity of Enterocytozoon bieneusi in Cervids from Milu Park in Beijing, China

SIMPLE SUMMARY: Enterocytozoon bieneusi is the most prevalent microsporidian species that can cause significant intestinal diseases in both humans and animals worldwide. This study investigated the overall E. bieneusi prevalence of 21.9% (47/215) in captive and free-ranging deer species in Beijing,...

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Autores principales: Zhang, Qingxun, Zhong, Zhenyu, Xia, Zhiqiang, Meng, Qinghui, Shan, Yunfang, Guo, Qingyun, Cheng, Zhibin, Zhang, Peiyang, He, Hongxuan, Bai, Jiade
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219422/
https://www.ncbi.nlm.nih.gov/pubmed/35739875
http://dx.doi.org/10.3390/ani12121539
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author Zhang, Qingxun
Zhong, Zhenyu
Xia, Zhiqiang
Meng, Qinghui
Shan, Yunfang
Guo, Qingyun
Cheng, Zhibin
Zhang, Peiyang
He, Hongxuan
Bai, Jiade
author_facet Zhang, Qingxun
Zhong, Zhenyu
Xia, Zhiqiang
Meng, Qinghui
Shan, Yunfang
Guo, Qingyun
Cheng, Zhibin
Zhang, Peiyang
He, Hongxuan
Bai, Jiade
author_sort Zhang, Qingxun
collection PubMed
description SIMPLE SUMMARY: Enterocytozoon bieneusi is the most prevalent microsporidian species that can cause significant intestinal diseases in both humans and animals worldwide. This study investigated the overall E. bieneusi prevalence of 21.9% (47/215) in captive and free-ranging deer species in Beijing, China. Thirteen E. bieneusi genotypes including six known and seven novel genotypes were identified. These resources will provide the insights to understand the veterinary and public health and the transmission dynamics between animal environments and human ones. ABSTRACT: Enterocytozoon bieneusi is the most prevalent microsporidian species that can cause zoonotic diseases in humans and animals. Despite receiving increasing attention in relation to domestic animals, there has been limited information on the infection burden of E. bieneusi in cervids. Altogether, 215 fecal samples collected from four deer species in Beijing, China were examined by nested- Polymerase Chain Reaction (PCR)targeting the internal transcribed spacer (ITS) region. The overall prevalence of E. bieneusi in deer was 21.9% (47/215), with 30.0% (24/80) in Pere David’s deer, 27.3% (15/55) in fallow deer, 12.5% (5/40) in sika deer, and 7.5% (3/40) in Chinese water deer. Thirteen E. bieneusi genotypes were identified, including six known (HLJD-V, MWC_d1, BEB6, CGC2, JLD-XV, and HND-I) and seven novel genotypes (BJED-I to BJED-V, BJFD, and BJCWD). A phylogenetic analysis showed that 38.3% of the isolates belonged to zoonotic Group 1. In addition, E. bieneusi infection was first detected in fallow deer and Chinese water deer, which could act as potential zoonotic reservoirs. Our findings suggest that E. bieneusi circulates in deer and might be of importance to public health.
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spelling pubmed-92194222022-06-24 Molecular Epidemiology and Genetic Diversity of Enterocytozoon bieneusi in Cervids from Milu Park in Beijing, China Zhang, Qingxun Zhong, Zhenyu Xia, Zhiqiang Meng, Qinghui Shan, Yunfang Guo, Qingyun Cheng, Zhibin Zhang, Peiyang He, Hongxuan Bai, Jiade Animals (Basel) Article SIMPLE SUMMARY: Enterocytozoon bieneusi is the most prevalent microsporidian species that can cause significant intestinal diseases in both humans and animals worldwide. This study investigated the overall E. bieneusi prevalence of 21.9% (47/215) in captive and free-ranging deer species in Beijing, China. Thirteen E. bieneusi genotypes including six known and seven novel genotypes were identified. These resources will provide the insights to understand the veterinary and public health and the transmission dynamics between animal environments and human ones. ABSTRACT: Enterocytozoon bieneusi is the most prevalent microsporidian species that can cause zoonotic diseases in humans and animals. Despite receiving increasing attention in relation to domestic animals, there has been limited information on the infection burden of E. bieneusi in cervids. Altogether, 215 fecal samples collected from four deer species in Beijing, China were examined by nested- Polymerase Chain Reaction (PCR)targeting the internal transcribed spacer (ITS) region. The overall prevalence of E. bieneusi in deer was 21.9% (47/215), with 30.0% (24/80) in Pere David’s deer, 27.3% (15/55) in fallow deer, 12.5% (5/40) in sika deer, and 7.5% (3/40) in Chinese water deer. Thirteen E. bieneusi genotypes were identified, including six known (HLJD-V, MWC_d1, BEB6, CGC2, JLD-XV, and HND-I) and seven novel genotypes (BJED-I to BJED-V, BJFD, and BJCWD). A phylogenetic analysis showed that 38.3% of the isolates belonged to zoonotic Group 1. In addition, E. bieneusi infection was first detected in fallow deer and Chinese water deer, which could act as potential zoonotic reservoirs. Our findings suggest that E. bieneusi circulates in deer and might be of importance to public health. MDPI 2022-06-14 /pmc/articles/PMC9219422/ /pubmed/35739875 http://dx.doi.org/10.3390/ani12121539 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Qingxun
Zhong, Zhenyu
Xia, Zhiqiang
Meng, Qinghui
Shan, Yunfang
Guo, Qingyun
Cheng, Zhibin
Zhang, Peiyang
He, Hongxuan
Bai, Jiade
Molecular Epidemiology and Genetic Diversity of Enterocytozoon bieneusi in Cervids from Milu Park in Beijing, China
title Molecular Epidemiology and Genetic Diversity of Enterocytozoon bieneusi in Cervids from Milu Park in Beijing, China
title_full Molecular Epidemiology and Genetic Diversity of Enterocytozoon bieneusi in Cervids from Milu Park in Beijing, China
title_fullStr Molecular Epidemiology and Genetic Diversity of Enterocytozoon bieneusi in Cervids from Milu Park in Beijing, China
title_full_unstemmed Molecular Epidemiology and Genetic Diversity of Enterocytozoon bieneusi in Cervids from Milu Park in Beijing, China
title_short Molecular Epidemiology and Genetic Diversity of Enterocytozoon bieneusi in Cervids from Milu Park in Beijing, China
title_sort molecular epidemiology and genetic diversity of enterocytozoon bieneusi in cervids from milu park in beijing, china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219422/
https://www.ncbi.nlm.nih.gov/pubmed/35739875
http://dx.doi.org/10.3390/ani12121539
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