Toxocara canis Infection Alters mRNA Expression Profiles of Peripheral Blood Mononuclear Cells in Beagle Dogs at the Lung Infection Period

SIMPLE SUMMARY: Toxocariasis is one of the most neglected zoonoses in the world. Toxocara canis is the main pathogen causing toxocariasis in humans and animals, threatening public health. To date, the mechanism by which the larvae of T. canis escape the attack of immune cells in the blood is still poorly understood. Using RNA-seq technology, the transcriptional alterations of Beagle dog peripheral blood mononuclear cells (PBMCs) between the presence and absence of T. canis infection were analyzed during the lung infection period, and 1066 upregulated genes and 1076 downregulated genes were identified (padj < 0.05 and |log(2) (FoldChange)| > 1). In addition, many immune- or inflammation-related GO terms and KEGG signaling pathways were significantly altered during T. canis infection by GO annotation and KEGG enrichment analysis. The present study revealed that T. canis infection can alter the mRNA profiles of PBMCs in Beagle dogs during the lung infection period, which has important implications for a better understanding of the interaction mechanism between T. canis and host immune cells. ABSTRACT: Toxocara canis is a neglected zoonotic roundworm distributed all over the world, causing toxocariasis in humans and animals. However, so far, the immune mechanism of T. canis infection in definitive hosts remains to be clarified. In this study, the transcriptional alterations of Beagle dogs’ peripheral blood mononuclear cells (PBMCs) induced by T. canis infection during the lung infection period were analyzed using RNA-seq technology. A total of 2142 differentially expressed genes were identified, with 1066 upregulated genes and 1076 downregulated genes. Many differentially expressed genes participated in the biological process of intracellular signal transduction, as well as the immune- or inflammation-related KEGG signaling pathway, such as the Notch signaling pathway, Toll-like receptor signaling pathway, and NF-kappa B signaling pathway, through KEGG enrichment analysis. This study indicated that T. canis infection could suppress the biological function of Beagle dogs’ PMBCs and provided basic data to further clarify the interaction mechanism between T. canis and host immune cells.