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The Microbiota and Cytokines Correlation between the Jejunum and Colon in Altay Sheep

SIMPLE SUMMARY: Both the jejunum and the colon secrete unique immune factors that interact with the gut microbiota. Investigating the association of gut microbiota and the host immune system, we detected higher populations of Bacteroides, Fibrobacteres and Spirochetes in the colon than in the jejunu...

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Autores principales: Ye, Mengjun, Hou, Meng, Peng, Qimin, Jia, Sheng, Peng, Bin, Yin, Fangfang, Li, Na, Wang, Jinquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219508/
https://www.ncbi.nlm.nih.gov/pubmed/35739900
http://dx.doi.org/10.3390/ani12121564
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author Ye, Mengjun
Hou, Meng
Peng, Qimin
Jia, Sheng
Peng, Bin
Yin, Fangfang
Li, Na
Wang, Jinquan
author_facet Ye, Mengjun
Hou, Meng
Peng, Qimin
Jia, Sheng
Peng, Bin
Yin, Fangfang
Li, Na
Wang, Jinquan
author_sort Ye, Mengjun
collection PubMed
description SIMPLE SUMMARY: Both the jejunum and the colon secrete unique immune factors that interact with the gut microbiota. Investigating the association of gut microbiota and the host immune system, we detected higher populations of Bacteroides, Fibrobacteres and Spirochetes in the colon than in the jejunum of Altay sheep, which is a unique breed in Xinjiang. Levels of IL-6 and IL-12 were lower in the colon than in the jejunum. IL-10 was positively correlated with Ruminococcus_2 in the jejunum. These results indicate a potential interaction between intestinal microbiota and the host immune system that may be considered for the prevention of sheep diseases and the screening of probiotics. ABSTRACT: Both the jejunum and colon release cytokines that interact with intestinal microbiota. However, it is largely unclear which cytokines and microbial populations are involved in the homeostasis of the intestinal ecosystem for sheep health. To address this, we collected contents for isolating microbiota and tissues for determining cytokines from the jejunum and colon of 7-month-old Altay sheep. We used the techniques of 16S rRNA sequencing and ELISA to detect microbial population and cytokine level, respectively. Correlations between microbial population and cytokines were analyzed by Spearman correlation coefficient. The correlation analysis revealed higher populations of Bacteroides, Fibrobacteres and Spirochetes in the colon than in the jejunum, and IL-6 and IL-12 levels were higher in the jejunum than in the colon. Association analysis further revealed a positive association between IL-10 level and both Ruminococcus_2 and norank_f_Bifidobacteriaceae population in the jejunum. The analysis also revealed positive associations between IL-6 level and Ruminococcaceae_UCG-014 and Ruminococcaceae_UCG-013 population, IL-10 and Prevotellaceae_UCG-004, as well as TNF-α and Prevotellaceae_UCG-003 in the colon. These results indicate a potential interaction between the intestinal microbiota and the host immune system that needs to be further clarified for considering dietary formulations to maintain animal health and disease prevention.
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spelling pubmed-92195082022-06-24 The Microbiota and Cytokines Correlation between the Jejunum and Colon in Altay Sheep Ye, Mengjun Hou, Meng Peng, Qimin Jia, Sheng Peng, Bin Yin, Fangfang Li, Na Wang, Jinquan Animals (Basel) Article SIMPLE SUMMARY: Both the jejunum and the colon secrete unique immune factors that interact with the gut microbiota. Investigating the association of gut microbiota and the host immune system, we detected higher populations of Bacteroides, Fibrobacteres and Spirochetes in the colon than in the jejunum of Altay sheep, which is a unique breed in Xinjiang. Levels of IL-6 and IL-12 were lower in the colon than in the jejunum. IL-10 was positively correlated with Ruminococcus_2 in the jejunum. These results indicate a potential interaction between intestinal microbiota and the host immune system that may be considered for the prevention of sheep diseases and the screening of probiotics. ABSTRACT: Both the jejunum and colon release cytokines that interact with intestinal microbiota. However, it is largely unclear which cytokines and microbial populations are involved in the homeostasis of the intestinal ecosystem for sheep health. To address this, we collected contents for isolating microbiota and tissues for determining cytokines from the jejunum and colon of 7-month-old Altay sheep. We used the techniques of 16S rRNA sequencing and ELISA to detect microbial population and cytokine level, respectively. Correlations between microbial population and cytokines were analyzed by Spearman correlation coefficient. The correlation analysis revealed higher populations of Bacteroides, Fibrobacteres and Spirochetes in the colon than in the jejunum, and IL-6 and IL-12 levels were higher in the jejunum than in the colon. Association analysis further revealed a positive association between IL-10 level and both Ruminococcus_2 and norank_f_Bifidobacteriaceae population in the jejunum. The analysis also revealed positive associations between IL-6 level and Ruminococcaceae_UCG-014 and Ruminococcaceae_UCG-013 population, IL-10 and Prevotellaceae_UCG-004, as well as TNF-α and Prevotellaceae_UCG-003 in the colon. These results indicate a potential interaction between the intestinal microbiota and the host immune system that needs to be further clarified for considering dietary formulations to maintain animal health and disease prevention. MDPI 2022-06-17 /pmc/articles/PMC9219508/ /pubmed/35739900 http://dx.doi.org/10.3390/ani12121564 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ye, Mengjun
Hou, Meng
Peng, Qimin
Jia, Sheng
Peng, Bin
Yin, Fangfang
Li, Na
Wang, Jinquan
The Microbiota and Cytokines Correlation between the Jejunum and Colon in Altay Sheep
title The Microbiota and Cytokines Correlation between the Jejunum and Colon in Altay Sheep
title_full The Microbiota and Cytokines Correlation between the Jejunum and Colon in Altay Sheep
title_fullStr The Microbiota and Cytokines Correlation between the Jejunum and Colon in Altay Sheep
title_full_unstemmed The Microbiota and Cytokines Correlation between the Jejunum and Colon in Altay Sheep
title_short The Microbiota and Cytokines Correlation between the Jejunum and Colon in Altay Sheep
title_sort microbiota and cytokines correlation between the jejunum and colon in altay sheep
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219508/
https://www.ncbi.nlm.nih.gov/pubmed/35739900
http://dx.doi.org/10.3390/ani12121564
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