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Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece
SIMPLE SUMMARY: The objective of this study was to investigate, for the first time in Greece, the prevalence of ESBL producers in swine populations and to correlate their occurrence with risk factors. A total of 214 fecal samples were collected from the farms from December 2019 to April 2021. A subs...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219512/ https://www.ncbi.nlm.nih.gov/pubmed/35739896 http://dx.doi.org/10.3390/ani12121560 |
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author | Tsekouras, Nikolaos Athanasakopoulou, Zoi Diezel, Celia Kostoulas, Polychronis Braun, Sascha D. Sofia, Marina Monecke, Stefan Ehricht, Ralf Chatzopoulos, Dimitris C. Gary, Dominik Krähmer, Domenique Spyrou, Vassiliki Christodoulopoulos, Georgios Billinis, Charalambos Papatsiros, Vasileios G. |
author_facet | Tsekouras, Nikolaos Athanasakopoulou, Zoi Diezel, Celia Kostoulas, Polychronis Braun, Sascha D. Sofia, Marina Monecke, Stefan Ehricht, Ralf Chatzopoulos, Dimitris C. Gary, Dominik Krähmer, Domenique Spyrou, Vassiliki Christodoulopoulos, Georgios Billinis, Charalambos Papatsiros, Vasileios G. |
author_sort | Tsekouras, Nikolaos |
collection | PubMed |
description | SIMPLE SUMMARY: The objective of this study was to investigate, for the first time in Greece, the prevalence of ESBL producers in swine populations and to correlate their occurrence with risk factors. A total of 214 fecal samples were collected from the farms from December 2019 to April 2021. A subset of 78 (78/214, 36.5%) ESBL producers were identified as Escherichia coli (E. coli, 88.5%), Klebsiella pneumoniae spp. pneumoniae (K. pneumoniae, 3.8%), Proteus mirabilis (P. mirabilis, 5.1%), Enterobacter cloacae complex (E. cloacae complex, 1.3%) and Salmonella enterica spp. diarizonae (S. enterica spp. diarizonae, 1.3%). CTX-M, SHV and TEM genes were detected along with genes conferring resistance to fluoroquinolones, aminoglycosides, sulfonamides, trimethoprim, macrolides and colistin. This study displayed high antimicrobial resistance rates in the Greek swine industry, and our results are alarming for both human and animal health. ABSTRACT: This study aimed to estimate the prevalence of extended-spectrum β-lactamase-producing (ESBL) bacteria in swine. Thus, 214 fecal samples were collected from suckling and weaned piglets from 34 farms in Greece (out of an overall population of about 14,300 sows). A subset of 78 (36.5%) ESBL producers were identified as E. coli (69/78, 88.5%), K. pneumoniae spp. pneumoniae (3.8%), P. mirabilis (5.1%), E. cloacae complex (1.3%) and S. enterica spp. diarizonae (1.3%). Resistance to at least one class of non-β-lactam antibiotics was detected in 78 isolates. Among the E. coli strains, resistance was identified with regard to aminoglycosides (n = 31), fluoroquinolones (n = 49), tetracycline (n = 26) and trimethoprim/sulfamethoxazole (n = 46). Of the three K. pneumoniae spp. pneumoniae, two displayed resistances to aminoglycosides and all were resistant to fluoroquinolones, tetracyclines and trimethoprim/sulfamethoxazole. As for the four P. mirabilis isolates, three had a resistant phenotype for aminoglycosides and all were resistant to imipenem, fluoroquinolones, tetracyclines and trimethoprim/sulfamethoxazole. Molecular characterization of the isolates revealed the presence of CTX-M, SHV and TEM genes, as well as of genes conferring resistance to fluoroquinolones, aminoglycosides, sulfonamides, trimethoprim, macrolides and colistin. High levels of antimicrobial resistance (AMR) were demonstrated in Greek swine herds posing a concern for the efficacy of treatments at the farm level as well as for public health. |
format | Online Article Text |
id | pubmed-9219512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92195122022-06-24 Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece Tsekouras, Nikolaos Athanasakopoulou, Zoi Diezel, Celia Kostoulas, Polychronis Braun, Sascha D. Sofia, Marina Monecke, Stefan Ehricht, Ralf Chatzopoulos, Dimitris C. Gary, Dominik Krähmer, Domenique Spyrou, Vassiliki Christodoulopoulos, Georgios Billinis, Charalambos Papatsiros, Vasileios G. Animals (Basel) Article SIMPLE SUMMARY: The objective of this study was to investigate, for the first time in Greece, the prevalence of ESBL producers in swine populations and to correlate their occurrence with risk factors. A total of 214 fecal samples were collected from the farms from December 2019 to April 2021. A subset of 78 (78/214, 36.5%) ESBL producers were identified as Escherichia coli (E. coli, 88.5%), Klebsiella pneumoniae spp. pneumoniae (K. pneumoniae, 3.8%), Proteus mirabilis (P. mirabilis, 5.1%), Enterobacter cloacae complex (E. cloacae complex, 1.3%) and Salmonella enterica spp. diarizonae (S. enterica spp. diarizonae, 1.3%). CTX-M, SHV and TEM genes were detected along with genes conferring resistance to fluoroquinolones, aminoglycosides, sulfonamides, trimethoprim, macrolides and colistin. This study displayed high antimicrobial resistance rates in the Greek swine industry, and our results are alarming for both human and animal health. ABSTRACT: This study aimed to estimate the prevalence of extended-spectrum β-lactamase-producing (ESBL) bacteria in swine. Thus, 214 fecal samples were collected from suckling and weaned piglets from 34 farms in Greece (out of an overall population of about 14,300 sows). A subset of 78 (36.5%) ESBL producers were identified as E. coli (69/78, 88.5%), K. pneumoniae spp. pneumoniae (3.8%), P. mirabilis (5.1%), E. cloacae complex (1.3%) and S. enterica spp. diarizonae (1.3%). Resistance to at least one class of non-β-lactam antibiotics was detected in 78 isolates. Among the E. coli strains, resistance was identified with regard to aminoglycosides (n = 31), fluoroquinolones (n = 49), tetracycline (n = 26) and trimethoprim/sulfamethoxazole (n = 46). Of the three K. pneumoniae spp. pneumoniae, two displayed resistances to aminoglycosides and all were resistant to fluoroquinolones, tetracyclines and trimethoprim/sulfamethoxazole. As for the four P. mirabilis isolates, three had a resistant phenotype for aminoglycosides and all were resistant to imipenem, fluoroquinolones, tetracyclines and trimethoprim/sulfamethoxazole. Molecular characterization of the isolates revealed the presence of CTX-M, SHV and TEM genes, as well as of genes conferring resistance to fluoroquinolones, aminoglycosides, sulfonamides, trimethoprim, macrolides and colistin. High levels of antimicrobial resistance (AMR) were demonstrated in Greek swine herds posing a concern for the efficacy of treatments at the farm level as well as for public health. MDPI 2022-06-16 /pmc/articles/PMC9219512/ /pubmed/35739896 http://dx.doi.org/10.3390/ani12121560 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tsekouras, Nikolaos Athanasakopoulou, Zoi Diezel, Celia Kostoulas, Polychronis Braun, Sascha D. Sofia, Marina Monecke, Stefan Ehricht, Ralf Chatzopoulos, Dimitris C. Gary, Dominik Krähmer, Domenique Spyrou, Vassiliki Christodoulopoulos, Georgios Billinis, Charalambos Papatsiros, Vasileios G. Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece |
title | Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece |
title_full | Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece |
title_fullStr | Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece |
title_full_unstemmed | Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece |
title_short | Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece |
title_sort | cross-sectional survey of antibiotic resistance in extended spectrum β-lactamase-producing enterobacteriaceae isolated from pigs in greece |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219512/ https://www.ncbi.nlm.nih.gov/pubmed/35739896 http://dx.doi.org/10.3390/ani12121560 |
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